Tyrosine phosphorylation of a 34-kDa protein induced by cross-linking a novel glycosylphosphatidylinositol-anchored glycoprotein (GPI-80) on human neutrophils that may regulate their adherence and migration.

abstract：:Although the amino acid sequences and the structures of pyruvate kinase (PYK) isozymes are highly conserved, allosteric regulations differ. This suggests that amino acids with low conservation play important roles in the allosteric mechanism. The current work exploits a 'natural screen'- the 122 point mutations identi...

journal_title：IUBMB life

authors： Pendergrass DC,Williams R,Blair JB,Fenton AW

更新日期：2006-01-01 00:00:00

abstract：:This paper reviews our studies that have addressed the molecular mechanisms underlying the biosynthesis and reception of extracellular signaling molecules and integrative mechanisms of extracellular-intracellular signaling transmission in biological systems. We introduced recombinant DNA technology into the neuroendoc...

journal_title：IUBMB life

authors： Nakanishi S

更新日期：2006-05-01 00:00:00

abstract：:The ATP-sensitive potassium (K(ATP)) channel is expressed in most excitable tissues and plays a critical role in numerous physiological processes by coupling intracellular energetics to electrical activity. The channel is comprised of four Kir6.x subunits associated with four regulatory sulfonylurea receptors (SUR). I...

journal_title：IUBMB life

authors： Akrouh A,Halcomb SE,Nichols CG,Sala-Rabanal M

更新日期：2009-10-01 00:00:00

abstract：:The purpose of this study was to confirm protein-protein interaction between cyclooxygenase-1 (COX-1) and caveolins. The interaction of cyclooxygenase-1 and caveolins in the cultured human embryonic kidney (HEK 293) cells was investigated using immuno-precipitation and Western blot analysis. In HEK 293 cells, high lev...

journal_title：IUBMB life

authors： Cha SH,Jung NH,Kim BR,Kim HW,Kwak JO

更新日期：2004-04-01 00:00:00

abstract：:Recent structural studies of proteins mediating membrane fusion reveal intriguing similarities between diverse viral and mammalian systems. Particularly striking is the close similarity between the transmembrane envelope glycoproteins from the retrovirus HTLV-1 and the filovirus Ebola. These similarities suggest simil...

journal_title：IUBMB life

authors： Poumbourios P,Center RJ,Wilson KA,Kemp BE,Kobe B

更新日期：1999-08-01 00:00:00

abstract：:Deletion of tumor suppressor gene, lethal(2)giant larvae [l(2)gl], leads to brain tumor in Drosophila melanogaster at larval stage of development and severe brain dysplasia in mice. We have studied the effect of two potential antitumor drugs artemisinin and curcumin in the perspective of inhibiting l(2)gl brain tumor....

journal_title：IUBMB life

authors： Das SS,Nanda GG,Alone DP

更新日期：2014-07-01 00:00:00

abstract：:Our results demonstrate that open reading frame (ORF) YHR076w on chromosome VIII of Saccharomyces cerevisiae that was previously described as a hypothetical gene is expressed. This ORF is transcribed as an mRNA of approximately 1,100 nucleotides. A 41.2-kDa polypeptide and three others predicted to be modified forms o...

journal_title：IUBMB life

authors： Ramos CW,Güldener U,Klein S,Hegemann JH,Gonzalez S,Rodríguez-Medina JR

更新日期：2000-12-01 00:00:00

abstract：:Heparin and its various derivatives affect cancer progression in humans. In this study, we show that heparin uptaken intracellularly by melanoma cells activated a signaling cascade, which in turn inhibited melanoma cell adhesion and migration. The reduced ability of M5 cells to adhere onto the fibronectin (FN) substra...

journal_title：IUBMB life

authors： Chalkiadaki G,Nikitovic D,Berdiaki A,Katonis P,Karamanos NK,Tzanakakis GN

更新日期：2011-02-01 00:00:00

abstract：:In this article, we discuss single molecule observation of rotational catalysis by E. coli ATP synthase (F-ATPase) using small gold beads. Studies involving a low viscous drag probe showed the stochastic properties of the enzyme in alternating catalytically active and inhibited states. The importance of subunit intera...

journal_title：IUBMB life

authors： Nakanishi-Matsui M,Sekiya M,Futai M

更新日期：2013-03-01 00:00:00

abstract：:Nur77 is a member of nuclear receptor superfamily that acts as a transcription factor and regulates expression of multiple genes. Subcellular localization of Nur77 protein plays an important role in the survival and cell death. In this study, we have predicted and confirmed alternatively spliced two new transcripts of...

journal_title：IUBMB life

authors： Rehman SU,Sarwar T,Husain MA,Ishqi HM,Tabish M

更新日期：2017-02-01 00:00:00

abstract：:Pilins or fimbrilins are a class of proteins found in bacterial surface pilus, a hair-like surface appendage. Both the Gram-negative and -positive bacteria produce pilins to assemble pili on their cell-surface for different purposes including adherence, twitching motility, conjugation, immunomodulation, biofilm format...

journal_title：IUBMB life

authors： Krishnan V

更新日期：2015-07-01 00:00:00

abstract：:Renal ischemia/reperfusion (I/R) is an alternation of renal hemodynamics, which results in diverse postischemic responses and eventually acute kidney injury. Although renal ischemic preconditioning (IPC) is known to protect the kidney from I/R injury, the precise renoprotective mechanisms are not completely understood...

journal_title：IUBMB life

authors： Li JR,Ou YC,Wu CC,Wang JD,Lin SY,Wang YY,Chen WY,Chen CJ

更新日期：2019-03-01 00:00:00

abstract：:Silymarin, which is derived from the seeds of Silybum marianum, has been widely used to prevent and treat liver diseases. In our previous study, we reported that at concentrations above the minimal inhibitory concentration (MIC), silymarin exhibited antifungal activity against Candida albicans by targeting its plasma ...

journal_title：IUBMB life

authors： Lee W,Lee DG

更新日期：2018-03-01 00:00:00

abstract：:Alzheimer's disease (AD) is characterized by the presence of senile plaques and neurofibrillary tangles in the brain. The beta-amyloid peptide (Abeta) is the primary constituent of the senile plaques, and has been proposed to be a key contributor to the neurodegeneration observed in AD. The molecular mechanisms underl...

journal_title：IUBMB life

authors： Gong X,Lu X,Zhan L,Sui H,Qi X,Ji Z,Niu X,Liu L

更新日期：2010-03-01 00:00:00

abstract：:The functional effect of the interaction of E2F1 and hepatitis B virus X protein (HBx) on the promoter of human p53 gene was studied using chloramphenicol acetyl transferase (CAT) assay. E2F1 activated the p53 promoter through E2F1 binding site. As previously reported, HBx repressed the p53 promoter through E-box. Whe...

journal_title：IUBMB life

authors： Choi M,Lee H,Rho HM

更新日期：2002-06-01 00:00:00

abstract：:Cancers have a clonal origin, yet their chromosomes and genes are non-clonal or heterogeneous due to an inherent genomic instability. However, the cause of this genomic instability is still debated. One theory postulates that mutations in genes that are involved in DNA repair and in chromosome segregation are the prim...

journal_title：IUBMB life

authors： Duesberg P,Fabarius A,Hehlmann R

更新日期：2004-02-01 00:00:00

abstract：:Members of the GATA family of transcription factors play key roles in the differentiation of specific cell lineages by regulating the expression of target genes. Three GATA factors play distinct roles in hematopoietic differentiation. In order to better understand how these GATA factors function to regulate genes thro...

journal_title：IUBMB life

authors： Hardison RC,Zhang Y,Keller CA,Xiang G,Heuston EF,An L,Lichtenberg J,Giardine BM,Bodine D,Mahony S,Li Q,Yue F,Weiss MJ,Blobel GA,Taylor J,Hughes J,Higgs DR,Göttgens B

更新日期：2020-01-01 00:00:00

abstract：:Role of nitric oxide (NO) on the expression of organic anion transporter 2 (OAT2) located in sinusoidal domain of hepatocytes has been investigated. Effect of NO generated in vivo in rat and delivered in vitro to hepatocytes was determined. Lipopolysaccharide (LPS) and spermine NONOate (SPER/NO) were selected as the N...

journal_title：IUBMB life

authors： Cha SH,Kim HP,Jung NH,Lee WK,Kim JY,Cha YN

更新日期：2002-09-01 00:00:00

abstract：:Small extracellular vesicle (sEV) has precise impacts on tumor microenvironment and play vital functions in intercellular interaction. However, the functional role of sEV miRNA on laryngeal squamous cell carcinoma (LSCC) is largely unresolved. Here, the expression of miR-1246 in LSCC tissues and plasma sEV was examine...

journal_title：IUBMB life

authors： Huang Q,Hsueh CY,Guo Y,Wu XF,Li JY,Zhou L

更新日期：2020-07-01 00:00:00

abstract：:Peroxynitrite is an inorganic toxin of physiological interest, formed from the diffusion-controlled reaction of superoxide and nitrogen monoxide with a rate constant of (1.6 +/- 0.3) x 10(10) M(-1) s(-1). On the basis of three experiments we conclude that homolysis of the O-O bond in peroxynitrous acid is unlikely: (1...

journal_title：IUBMB life

authors： Kissner R,Nauser T,Kurz C,Koppenol WH

更新日期：2003-10-01 00:00:00

abstract：:Endothelial dysfunction is fundamental to ischemic stroke and brain injury. The humanin analogue S14G-humanin (HNG) has been shown to be a cytoprotective derivative. In this study, we investigated the neuroprotective effects of HNG in vivo and in vitro. In a murine middle cerebral artery occlusion (MCAO) stroke model,...

journal_title：IUBMB life

authors： Peng T,Wan W,Wang J,Liu Y,Fu Z,Ma X,Li J,Sun G,Ji Y,Lu J,Lu H,Liu Y

更新日期：2018-07-01 00:00:00

abstract：:Transfected human apoptosis signal-regulating kinase 1 (ASK1) produces a 150 kDa protein. However, we have detected endogenous ASK1 predominantly as 39 and 50 kDa C-terminal and 75 and 110 kDa N-terminal fragments in a panel of nontransfected cancer cell lines and HUVEC endothelial cells. This suggests that in nonapop...

journal_title：IUBMB life

authors： Stordal B,Davey R

更新日期：2008-03-01 00:00:00

abstract：:The physiological role of neuroglobin and cytoglobin, two vertebrate globins discovered in the last 5 years, is not yet clearly understood. In this work, we review the structural information on these globins and its implication on the possible protein function, obtained by electron paramagnetic resonance and resonance...

journal_title：IUBMB life

authors： Van Doorslaer S,Vinck E,Trandafir F,Ioanitescu I,Dewilde S,Moens L

更新日期：2004-11-01 00:00:00

abstract：:Triclosan, a well-known inhibitor of Enoyl Acyl Carrier Protein Reductase (ENR) from several pathogenic organisms, is a promising lead compound to design effective drugs. We have solved the X-ray crystal structures of Plasmodium falciparum ENR in complex with triclosan variants having different substituted and unsubst...

journal_title：IUBMB life

authors： Maity K,Bhargav SP,Sankaran B,Surolia N,Surolia A,Suguna K

更新日期：2010-06-01 00:00:00

abstract：:Hepatitis C virus (HCV) is an enveloped positive-strand RNA virus of the Flaviviridae family. It has a genome of about 9,600 nucleotides encoding a large polyprotein (about 3,000 amino acids) that is processed by cellular and viral proteases into at least 10 structural and nonstructural viral proteins. A novel HCV pro...

journal_title：IUBMB life

authors： Vassilaki N,Mavromara P

更新日期：2009-07-01 00:00:00

abstract：:Shiga toxin (Stx) producing Escherichia coli (STEC) is responsible to bloody diarrhea (hemorrhagic colitis) and the hemolytic uremic syndrome (HUS). STEC strains carry inducible lambda phages integrated into their genomes that encode Stx 1 and/or 2, with several allelic variants each one. O157:H7 is the serotype that ...

journal_title：IUBMB life

authors： Ibarra C,Amaral MM,Palermo MS

更新日期：2013-10-01 00:00:00

abstract：:Release of arachidonic acid from membrane glycerophospholipids by cytosolic phospholipase A2 (cPLA2) is a key step in the generation of platelet-activating factor (PAF), recognized as the most proximal mediator of inflammatory events triggered by bacterial infection. Here, we report on the role of cPLA2 in the disturb...

journal_title：IUBMB life

authors： Slomiany BL,Slomiany A

更新日期：2006-04-01 00:00:00

abstract：:To further understand the role of the three conserved Val residues in insulin, B12Val, B18Val, and A3Val, five insulin mutants-[A3Ser]insulin, [B12Thr]insulin, (desB30)[B12Ser]insulin, [B18Thr] insulin, and [B18Leu]insulin--were obtained by means of site-directed mutagenesis and their receptor-binding activities as we...

journal_title：IUBMB life

authors： Guo ZY,Tang YH,Zhang Z,Feng YM

更新日期：2001-12-01 00:00:00

abstract：:Changes in the status of DNA methylation are one of the most common molecular alterations in human neoplasia. We aimed to identify epigenetic molecular markers in serum for early detection of breast cancer. Authors analyzed retrospectively the methylation status of RARβ2 and APC genes in serum samples from 121 breast ...

journal_title：IUBMB life

authors： Swellam M,Abdelmaksoud MD,Sayed Mahmoud M,Ramadan A,Abdel-Moneem W,Hefny MM

更新日期：2015-01-01 00:00:00

abstract：:Colorectal cancer (CRC) is one of the most common malignancies with poor prognosis. Oxaliplatin-based chemotherapy is an important treatment for CRC; however, the cells develop resistance to therapy. The mechanisms underlying oxaliplatin resistance are complex and unclear. There is increasing evidence that microRNAs (...

journal_title：IUBMB life

authors： Moradi Marjaneh R,Khazaei M,Ferns GA,Avan A,Aghaee-Bakhtiari SH

更新日期：2019-10-01 00:00:00