Abstract:
:Changes in the status of DNA methylation are one of the most common molecular alterations in human neoplasia. We aimed to identify epigenetic molecular markers in serum for early detection of breast cancer. Authors analyzed retrospectively the methylation status of RARβ2 and APC genes in serum samples from 121 breast cancer patients, 79 patients with benign breast diseases, and 66 healthy volunteers using methylation-specific PCR. The methylated APC and RARβ2 were significantly higher in breast cancer patients (93.4%, 95.6%) than benign (7.8%, 14.5%) but not detected in healthy volunteers (0%) at (P < 0.0001). Both methylated genes showed no significant difference among clinicopathological factors apart from triple negative breast cancer patients as all of them (χ(2) = 7.4, P = 0.007) reported to be methylated RARβ2 genes. Both methylated genes were detected in all grades and stages. Both sensitivities and specificities of the methylated genes for breast cancer detection were superior to traditional tumor markers in detection of breast cancer, early stage, low grade tumors, and triple negative breast cancer patients. Thus methylated APC and RARβ2 genes might be valuable serum-based molecular markers for early detection of breast cancer.
journal_name
IUBMB Lifejournal_title
IUBMB lifeauthors
Swellam M,Abdelmaksoud MD,Sayed Mahmoud M,Ramadan A,Abdel-Moneem W,Hefny MMdoi
10.1002/iub.1346subject
Has Abstractpub_date
2015-01-01 00:00:00pages
61-8issue
1eissn
1521-6543issn
1521-6551journal_volume
67pub_type
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