Abstract:
:In this article, we discuss single molecule observation of rotational catalysis by E. coli ATP synthase (F-ATPase) using small gold beads. Studies involving a low viscous drag probe showed the stochastic properties of the enzyme in alternating catalytically active and inhibited states. The importance of subunit interaction between the rotor and the stator, and thermodynamics of the catalysis are also discussed. "Single Molecule Enzymology" is a new trend for understanding enzyme mechanisms in biochemistry and physiology.
journal_name
IUBMB Lifejournal_title
IUBMB lifeauthors
Nakanishi-Matsui M,Sekiya M,Futai Mdoi
10.1002/iub.1134subject
Has Abstractpub_date
2013-03-01 00:00:00pages
247-54issue
3eissn
1521-6543issn
1521-6551journal_volume
65pub_type
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