Abstract:
:The mammalian antizyme (AZ) promotes ubiqutin-independent degradation of ornithine decarboxylase, a key enzyme in polyamine biosynthesis. This study shows that AZ suppression in human lung carcinoma A549 cells caused growth defects and death, but made the cells resistant to DNA damaging agents such as gamma-radiation and cisplatin. In these cells, the cellular redox potential (glutathione/glutathione disulfide [GSH/GSSG] ratio) was increased and thus intracellular reactive oxygen species were severely diminished, which might cause growth defects and cell death. The increase of cellular redox potential was mainly caused by dramatic increase of the cytoplasmic nicotinamide adenine dinucleotide phosphate (NADP)(+)-dependent isocitrate dehydrogenase, which generates the reducing equivalents NADPH. In the AZ-suppressed cells, the hypoxia inducible factor 1alpha (HIF-1alpha) was also increased. As in other cases which showed an increment of HIF-1alpha and the cellular redox potential, the AZ-suppressed cells showed resistance to gamma-radiation and anticancer drugs. Therefore, these facts might be considered as important for the use of radio- and chemotherapy on tumor cells which show an unbalance in their polyamine levels.
journal_name
IUBMB Lifejournal_title
IUBMB lifeauthors
Kim JS,Kim TL,Cho EW,Paik SG,Chung HW,Kim IGdoi
10.1002/iub.49subject
Has Abstractpub_date
2008-06-01 00:00:00pages
402-9issue
6eissn
1521-6543issn
1521-6551journal_volume
60pub_type
杂志文章相关文献
IUBMB LIFE文献大全abstract::PlncRNA-1 has been suggested to function as an oncogenic role in prostate cancer, colorectal cancer, hepatocellular carcinoma, esophageal squamous cell carcinoma, and gastric cancer. The expression pattern of PlncRNA-1 in retinoblastoma remained unknown. Therefore, the aim of this study was to explore the clinical sig...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.1886
更新日期:2018-10-01 00:00:00
abstract::Occlusion of a coronary artery if maintained for longer period of time results in damage of the cardiac tissue. However, restoration of blood flow to previously ischemic tissue can itself induce further cardiac damage, a phenomenon known as myocardial reperfusion injury. Cardiac homoeostasis is supported by a network ...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1002/iub.1352
更新日期:2015-02-01 00:00:00
abstract::Cooperative effects in ligand binding and dissociation kinetics are much less investigated than steady state kinetics or equilibrium binding. Nevertheless, cooperativity in ligand binding leads necessarily to characteristic properties with respect to kinetic properties of the system. In case of positive cooperativity ...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.459
更新日期:2011-05-01 00:00:00
abstract::We hypothesize that the various, previously reported, reactivities of neuroglobin with redox partners and oxygen provide for the establishment of a redox cycle within cells, such as neurons and retinal rod cells. Using native cell lysates, from cultured human cells of neuronal origin, we have estimated the rate of red...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.566
更新日期:2012-05-01 00:00:00
abstract::We have adapted the Java Molecular Viewer (JMV) to virtual reality display environments, through a number of extensions to the Java 3D code. Phylogenetic information derived from multiple alignments (temporal information) can be overlaid onto molecule structures (spatial information). The number of sequences included ...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/1521654031000136255
更新日期:2003-04-01 00:00:00
abstract::We propose a new hypothesis for the molecular mechanism by which neuroglobin exerts its protective effect in hypoxia-induced cell death. Our recent observation of a very rapid electron-transfer reaction between ferrous neuroglobin and ferric cytochrome c is central to this hypothesis. In contrast to previously suggest...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.35
更新日期:2008-06-01 00:00:00
abstract::In this study, the impact of pancreatic cancer cell interaction with macrophages on the differentiation and function of macrophages and the behaviors of pancreatic cancer cells in vitro is evaluated. The expression of immunocompetent cell-associated markers in 22 pancreatic cancer specimens was characterized by immuno...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.1336
更新日期:2014-12-01 00:00:00
abstract::Heparin and its various derivatives affect cancer progression in humans. In this study, we show that heparin uptaken intracellularly by melanoma cells activated a signaling cascade, which in turn inhibited melanoma cell adhesion and migration. The reduced ability of M5 cells to adhere onto the fibronectin (FN) substra...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.421
更新日期:2011-02-01 00:00:00
abstract::Calcineurin (CN) is the only serine/threonine specific protein phosphatase regulated by Ca(2+) /calmodulin (CaM), which is composed of catalytic A subunit (CNA) and regulatory B subunit (CNB). Tumor necrosis factor (TNF) receptor associated factor 3 (TRAF3) is an essential component in the Toll like receptors and TNF ...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.1060
更新日期:2012-09-01 00:00:00
abstract::The existence of a genetic component in mycobacterial disease susceptibility is no longer in doubt and the investigations now being conducted aim to determine which genes are involved, to what extent, and in which disease phenotype they are relevant. In certain rare instances of susceptibility to poorly pathogenic myc...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/15216540212644
更新日期:2002-04-01 00:00:00
abstract::Nur77 is a member of nuclear receptor superfamily that acts as a transcription factor and regulates expression of multiple genes. Subcellular localization of Nur77 protein plays an important role in the survival and cell death. In this study, we have predicted and confirmed alternatively spliced two new transcripts of...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.1605
更新日期:2017-02-01 00:00:00
abstract::Arginine biosynthesis pathway is crucial to the survival and pathogenesis of Mycobacterium tuberculosis (Mtb). Arginine is a critical amino acid that contributes to the inflection of cellular immune responses during pathogenesis. Argininosuccinate lyase from Mtb (MtArgH), the last enzyme in the pathway, catalyzes the ...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.1683
更新日期:2017-11-01 00:00:00
abstract:OBJECTIVE:Heart diseases are common in the offspring of gestational diabetic mother (ODM). Defects in mitochondrial metabolism and autophagy may, in part, be responsible for the adverse structural and functional alterations in the heart. The principal objective of this study was to investigate cardiac mitochondrial res...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.2449
更新日期:2021-01-22 00:00:00
abstract::To further understand the role of the three conserved Val residues in insulin, B12Val, B18Val, and A3Val, five insulin mutants-[A3Ser]insulin, [B12Thr]insulin, (desB30)[B12Ser]insulin, [B18Thr] insulin, and [B18Leu]insulin--were obtained by means of site-directed mutagenesis and their receptor-binding activities as we...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/152165401317291165
更新日期:2001-12-01 00:00:00
abstract::Shiga toxin (Stx) producing Escherichia coli (STEC) is responsible to bloody diarrhea (hemorrhagic colitis) and the hemolytic uremic syndrome (HUS). STEC strains carry inducible lambda phages integrated into their genomes that encode Stx 1 and/or 2, with several allelic variants each one. O157:H7 is the serotype that ...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1002/iub.1206
更新日期:2013-10-01 00:00:00
abstract::Peroxynitrite is an inorganic toxin of physiological interest, formed from the diffusion-controlled reaction of superoxide and nitrogen monoxide with a rate constant of (1.6 +/- 0.3) x 10(10) M(-1) s(-1). On the basis of three experiments we conclude that homolysis of the O-O bond in peroxynitrous acid is unlikely: (1...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/15216540310001628690
更新日期:2003-10-01 00:00:00
abstract::The cancer microenvironment and the interactions between cancer and surrounding tissue cells are thought to play a pivotal role in tumor development and progression. Glycosaminoglycans (GAGs)/proteoglycans (PGs) are major constituents of the extracellular matrix, the composition of which may affect various cellular fu...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.70
更新日期:2008-05-01 00:00:00
abstract::Methionine (Met) is a neutral, sulfur-containing, essential amino acid with biological functions in the initiation and prolongation step of protein synthesis, transmethylation reactions, the synthesis of cysteine and cystine, and as a component of antioxidant systems. Its key importance is reflected by the fact that i...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1002/iub.1571
更新日期:2016-12-01 00:00:00
abstract::Immune infiltration of tumors has been increasingly accepted as a prognostic factor in colon cancer. Here, we aim to develop a novel immune signature, based on estimated immune landscape from tumor transcriptomes, to predict the overall survival of patients with colon cancer. The compositions of 22 immune cell subtype...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.2124
更新日期:2019-11-01 00:00:00
abstract::The sodA gene coding for manganese superoxide dismutase (Mn-SOD) from the marine microorganism Vibrio parahaemolyticus was cloned, sequenced, and overexpressed in Escherichia coli by use of the pET20b (+) expression vector. The full-length gene consisted of a 588-bp open reading frame and encoded a polypeptide of 196 ...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/713803521
更新日期:1999-09-01 00:00:00
abstract::Availability of the human genome data has enabled the exploration of a huge amount of biological information encoded in it. There are extensive ongoing experimental efforts to understand the biological functions of the gene products encoded in the human genome. However, computational analysis can aid immensely in the ...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/15216540400006105
更新日期:2004-06-01 00:00:00
abstract::To establish possible cancerous aggressiveness between the metabolism of variously labeled [14C]glucose in the human breast invasive ductal carcinoma (IDC) tissues, we measured the rates of 14CO2 production from those tissues by using radiorespirometry, expressing the results as initial velocity (V) in nanomoles of 14...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/713803528
更新日期:1999-10-01 00:00:00
abstract::Deletion of tumor suppressor gene, lethal(2)giant larvae [l(2)gl], leads to brain tumor in Drosophila melanogaster at larval stage of development and severe brain dysplasia in mice. We have studied the effect of two potential antitumor drugs artemisinin and curcumin in the perspective of inhibiting l(2)gl brain tumor....
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.1284
更新日期:2014-07-01 00:00:00
abstract::Treatment of microsomes (preferably enriched with endoplasmic reticulum) isolated from bovine pulmonary artery smooth muscle tissue with the O2*- -generating system (hypoxanthine (HPX) plus xanthine oxidase (XO)), markedly stimulated matrix metalloproteinase-2 (MMP-2) activity and also enhanced Ca2+ ATPase activity an...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/1521654041000171335
更新日期:2004-05-01 00:00:00
abstract::The members of the GATA family of transcription factors have homologous zinc fingers and bind to similar sequence motifs. Recent advances in genome-wide technologies and the integration of bioinformatics data have led to a better understanding of how GATA factors regulate gene expression; GATA-factor-induced transcrip...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1002/iub.2169
更新日期:2020-01-01 00:00:00
abstract::The posttranscriptional regulation of miRNAs is important for organism development. To investigate the role of miRNAs in angiogenesis, we performed a loss-of-function screening assay in human umbilical vein endothelial cells (HUVECs) and found that knockdown of 7 miRNAs (miR-95a, miR-126, miR-129, miR-137, miR-139, mi...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.486
更新日期:2011-07-01 00:00:00
abstract::Prostate cancer (PCa) is considered the most common malignancy in men. The aim of this study is to assess the role of serum miR-15a and miR-16-1 expression in PCa development, diagnosis and prognosis aiming to find a specific noninvasive biomarker. This study comprised 70 patients with PCa, 70 patients complaining of ...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.1733
更新日期:2018-05-01 00:00:00
abstract::Nasal-type natural killer/T-cell lymphoma (NKTCL) is an aggressive malignancy with poor survival outcomes that is relatively resistant to chemotherapy. N6-Methyladenosine (m6A) modification, the most prevalent modification of eukaryotic messenger RNA, is involved in the progression of various tumors. However, it is un...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.2410
更新日期:2020-11-17 00:00:00
abstract::Myosin light chain kinase (MLCK) is a regulatory protein for smooth muscle contraction, which acts by phosphorylating 20-kDa myosin light chain (MLC20) to activate the myosin ATPase activity. Although this mode of action is well-established, there are numerous reports of smooth muscle contraction that is not associate...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/152165401753366087
更新日期:2001-06-01 00:00:00
abstract::In this article, the experiments used to construct the ambient pH-signaling network involved in the secretion of enzymes by filamentous fungi have been reviewed, focusing on the phosphate-repressible phosphatases in Aspergillus nidulans. Classic and molecular genetics have been used to demonstrate that proteolysis of ...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.1217
更新日期:2013-11-01 00:00:00