Abstract:
:Combining two analytical techniques, HPLC and liquid secondary ion mass spectrometry, the orientation of liposomal membrane-bound melittin was analyzed through its trypsin-digested products. We found that trypsin can access all proteolytic sites of the membrane-bound melittin when the liposomes have no transmembrane potential, whereas the proteolytic site near the N terminus of melittin is blocked when the liposomes have a negative transmembrane potential. The results suggest that the negative transmembrane potential may induce the melittin molecules to insert into the membrane perpendicularly, whereas melittin lies flat on the membrane surface in the absence of a negative potential.
journal_name
IUBMB Lifejournal_title
IUBMB lifeauthors
Niu W,Wu Y,Sui SFdoi
10.1080/152165400300001543keywords:
subject
Has Abstractpub_date
2000-09-01 00:00:00pages
215-9issue
3eissn
1521-6543issn
1521-6551journal_volume
50pub_type
杂志文章相关文献
IUBMB LIFE文献大全abstract::The complex life cycle of intracellular parasitic protozoans entails multiple rounds of DNA replication and mitosis followed by cytokinesis to release daughter parasites. To gain insights into mitotic events it is imperative to identify the biomarkers that constitute the chromosome segregation machinery in the parasit...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1002/iub.1878
更新日期:2018-08-01 00:00:00
abstract::In this study, the impact of pancreatic cancer cell interaction with macrophages on the differentiation and function of macrophages and the behaviors of pancreatic cancer cells in vitro is evaluated. The expression of immunocompetent cell-associated markers in 22 pancreatic cancer specimens was characterized by immuno...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.1336
更新日期:2014-12-01 00:00:00
abstract::In most organisms, productive utilization of galactose requires the highly conserved Leloir pathway of galactose metabolism. Yet, if this metabolic pathway is perturbed due to congenital deficiencies of the three associated enzymes, or an overwhelming presence of galactose, this monosaccharide which is abundantly pres...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1002/iub.262
更新日期:2009-11-01 00:00:00
abstract::The activation and incorporation of selenium into selenocysteine containing selenoproteins has been well established in an Escherichia coli model system but there is little specific information concerning the transport and intracellular trafficking of selenium in biological systems in general. A selenium transport rol...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/15216540400010818
更新日期:2004-08-01 00:00:00
abstract::Acid fibroblast growth factor (aFGF) binds to its cell-surface receptors in a heparin-dependent manner. In an attempt to define the aFGF recognition site on fibroblast growth factor receptor 1 (FGFR1), we developed a screening strategy for identifying FGF ligands that bind to the receptor-binding region of FGF. To ret...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/15216540050167106
更新日期:2000-06-01 00:00:00
abstract::Myosin light chain kinase (MLCK) is a regulatory protein for smooth muscle contraction, which acts by phosphorylating 20-kDa myosin light chain (MLC20) to activate the myosin ATPase activity. Although this mode of action is well-established, there are numerous reports of smooth muscle contraction that is not associate...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/152165401753366087
更新日期:2001-06-01 00:00:00
abstract::Prostate specific antigen (PSA) is a serine protease that is exclusively produced in the prostate, and its detection is the only laboratory test available for screening men for prostate cancer (PC). The interpretation of the assay is difficult since it is specific for prostate tissue and cellular growth, but not for P...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1002/iub.355
更新日期:2010-08-01 00:00:00
abstract::Fascin actin-bundling protein 1 (FSCN1) is an evolutionarily conserved actin-bundling protein that plays a critical role in cell migration, motility, adhesion, and cellular interactions. Although multiple clinical studies have implicated the expression of FSCN1 in laryngeal squamous cell carcinoma (LSCC) progression, ...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.2121
更新日期:2019-11-01 00:00:00
abstract::Carbon monoxide (CO) is a gaseous transmitter that is known to be involved in several physiological processes, but surprisingly it is also becoming a promising molecule to treat several pathologies including stroke and cancer. CO can cross the plasma membrane and activate guanylate cyclase, increasing the cGMP concent...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1002/iub.1388
更新日期:2015-06-01 00:00:00
abstract::Large inter-individual variation exists in the response to vitamin E supplementation, and this may influence the outcome of human studies. It is our hypothesis that genetic heterogeneity is an important determinant of vitamin E homeostasis. Therefore we have performed an in silico search for single nucleotide polymorp...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/15216540400020346
更新日期:2004-10-01 00:00:00
abstract::Implantation is a complex developmental event that is initiated by recognition and adhesion of the embryo to the endometrial epithelium. sLeX is an oligosaccharide antigen acting as the ligand of L-selectin, and is stage-specifically expressed in the endometrial epithelium. The adhesion system mediated by L-selectin a...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.62
更新日期:2008-07-01 00:00:00
abstract::Researchers have started focusing on investigating the anticarcinogenic effects of natural products with the slightest side effects possible, because current breast cancer treatment approaches are unable to achieve absolute success especially on aggressive subtypes. Propolis is among these products with its antimicrob...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.1996
更新日期:2019-05-01 00:00:00
abstract::The ATP-sensitive potassium (K(ATP)) channel is expressed in most excitable tissues and plays a critical role in numerous physiological processes by coupling intracellular energetics to electrical activity. The channel is comprised of four Kir6.x subunits associated with four regulatory sulfonylurea receptors (SUR). I...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1002/iub.246
更新日期:2009-10-01 00:00:00
abstract::Extracellular matrix (ECM)-independent survival is an essential prerequisite for tumor metastasis, and a hallmark of epithelial cancer stem cells and epithelial-mesenchymal transition (EMT). Here, we found that loss of TP53I11 enhanced, and overexpression of TP53I11 suppressed the ECM-independent survival, EMT, and mi...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.1949
更新日期:2019-02-01 00:00:00
abstract::Mitochondria play a key role in death signaling. The intermembrane space of these organelles contains a number of proteins which promote cell death once they are redistributed to the cytosol. The formation of pores in the outer membrane of mitochondria defines a gateway through which the apoptogenic proteins pass duri...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1002/iub.44
更新日期:2008-06-01 00:00:00
abstract::Transforming growth factorbeta1 (TGFbeta1) is considered to be the principal contributor to liver fibrosis. So in this study the ribozymes against TGFbeta1 were designed. The in vitro cleavage activities of the ribozymes were assayed through incubation of (32)p-labeled target RNAs and (32)p-labeled ribozymes in differ...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/15216540400024470
更新日期:2005-01-01 00:00:00
abstract::This study examined whether cell cycle regulatory proteins, such as cyclin-dependent kinases (CDKs), cyclins, and CDK inhibitors, regulate type II collagen expression and mediate interlukin-1 (IL-1beta)-induced suppression of type II collagen expression in articular chondrocytes. IL-1beta inhibited type II collagen ex...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/15216540701245022
更新日期:2007-02-01 00:00:00
abstract::The sodA gene coding for manganese superoxide dismutase (Mn-SOD) from the marine microorganism Vibrio parahaemolyticus was cloned, sequenced, and overexpressed in Escherichia coli by use of the pET20b (+) expression vector. The full-length gene consisted of a 588-bp open reading frame and encoded a polypeptide of 196 ...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/713803521
更新日期:1999-09-01 00:00:00
abstract::This paper reviews our studies that have addressed the molecular mechanisms underlying the biosynthesis and reception of extracellular signaling molecules and integrative mechanisms of extracellular-intracellular signaling transmission in biological systems. We introduced recombinant DNA technology into the neuroendoc...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/15216540600746385
更新日期:2006-05-01 00:00:00
abstract::Polycystic ovary syndrome (PCOS) is one of the most common endocrine-metabolic disorders; however, its pathophysiology is still unclear. Certain polymorphisms of luteinizing hormone beta-subunit (LHβ) and LH/choriogonadotrophin receptor (LHCGR) genes may lead to changes in the bioactivity of this hormone. We aimed to ...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.1457
更新日期:2016-01-01 00:00:00
abstract::The pathophysiology of some inflammatory arterial diseases (such as vasculitis, atherogenesis and aneurysms) has been widely investigated in the last decades. Among different soluble molecules, proinflammatory cytokines (such as TNF-α, IL-1 and IL-6) were shown to trigger critical pathways regulating these arterial di...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1002/iub.1344
更新日期:2015-01-01 00:00:00
abstract::Cardiac hypoxia plays a significant role in various types of heart disease, and improper treatment of hypoxia often leads to myocardial cell damage or even death. Transcriptome profiling and open chromatin mapping have been used as powerful tools to understand the development of heart disease, but the interplay betwee...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.2297
更新日期:2020-08-01 00:00:00
abstract::Specific RNAs can function as sinks for endogenous miRNAs, known as competing endogenous RNAs (ceRNAs). Here, we confirm a miR-124 mediated ceRNA crosstalk between LAMC1 and CD151 in hepatocellular carcinoma (HCC). miR-124 negatively regulates LAMC1 expression through two miRNA binding sites within its 3' untranslated...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.1642
更新日期:2017-08-01 00:00:00
abstract::In Streptomyces fradiae, calcium ions induce alterations in intensity and specificity of the secondary metabolism and stimulate sporulation. Using in vivo labeling, we demonstrate that in S. fradiae phosphorylation of some proteins are also influenced by Ca2+ added exogenously. Calcium ions at physiological concentrat...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/713803699
更新日期:2000-08-01 00:00:00
abstract::Endothelial dysfunction is fundamental to ischemic stroke and brain injury. The humanin analogue S14G-humanin (HNG) has been shown to be a cytoprotective derivative. In this study, we investigated the neuroprotective effects of HNG in vivo and in vitro. In a murine middle cerebral artery occlusion (MCAO) stroke model,...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.1869
更新日期:2018-07-01 00:00:00
abstract::Insulin-like growth factor-1 (IGF-1) signaling is a key pathway in the control of cell growth and survival. Three critical nodes in the IGF-1 signaling pathway have been described in cardiomyocytes: protein kinase Akt/mammalian target of rapamycin (mTOR), Ras/Raf/extracellular signal-regulated kinase (ERK), and phosph...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1002/iub.1172
更新日期:2013-07-01 00:00:00
abstract::This study was expected to reveal the regulatory effects of lncRNA UCA1 on pancreatic cancer cell progression through targeting miR-96/FOXO3. Microarray analysis was carried out on 36 cases of pancreatic cancer tissues and 16 cases of adjacent tissues among them. Expression levels of lncRNA UCA1, miR-96, and FOXO3 in ...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.1699
更新日期:2018-04-01 00:00:00
abstract::Copper has a wide and important role in biological systems, determining conformation and activity of many metalloproteins and enzymes, such as cytochrome oxidase and superoxide dismutase . Furthermore, due to its possible reactivity with nonspecific proteins and toxic effects, elaborate systems of absorption, concentr...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1002/iub.1590
更新日期:2017-04-01 00:00:00
abstract::Small extracellular vesicle (sEV) has precise impacts on tumor microenvironment and play vital functions in intercellular interaction. However, the functional role of sEV miRNA on laryngeal squamous cell carcinoma (LSCC) is largely unresolved. Here, the expression of miR-1246 in LSCC tissues and plasma sEV was examine...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.2274
更新日期:2020-07-01 00:00:00
abstract::Pilins or fimbrilins are a class of proteins found in bacterial surface pilus, a hair-like surface appendage. Both the Gram-negative and -positive bacteria produce pilins to assemble pili on their cell-surface for different purposes including adherence, twitching motility, conjugation, immunomodulation, biofilm format...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1002/iub.1400
更新日期:2015-07-01 00:00:00