Abstract:
:In this study, two different epitope tags (HA, c-myc) were introduced near the N terminus of the yeast PMA1 H(+)-ATPase. The resulting proteins were indistinguishable from the wild-type ATPase in their ability to travel through the secretory pathway, as judged by quantitative immunoblotting of isolated secretory vesicles. Furthermore, there were no significant abnormalities in ATPase activity (including K(m) for MgATP, Vmax, pH optimum, and IC50 for inhibition by vanadate) or in ATP-dependent proton pumping. Finally, the epitope-tagged ATPases could support normal growth and displayed the expected activation by glucose.
journal_name
IUBMB Lifejournal_title
IUBMB lifeauthors
dos Santos CF,DeWitt ND,Gupta SS,Allen KE,Slayman CWdoi
10.1080/15216540050022494keywords:
subject
Has Abstractpub_date
2000-02-01 00:00:00pages
153-9issue
2eissn
1521-6543issn
1521-6551journal_volume
49pub_type
杂志文章相关文献
IUBMB LIFE文献大全abstract::Red blood cells of yellow-spotted river turtles (Podocnemis unifilis, Pleurodira, Chelonia, REPTILIA) have two hemoglobin (Hb) components, Hb A and Hb D. We purified the hemoglobin component homologous to amniote (reptiles, birds, and mammals) adult Hb A which comprises two identical α(A) -globin polypeptides and two ...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.429
更新日期:2011-03-01 00:00:00
abstract::A pool of Plasmodium falciparum casein kinase 1 (PfCK1) has been shown to localize to the host red blood cell (RBC) membrane and be secreted to the extracellular medium during trophozoite stage of development. We attempted to identify mechanisms for secretion of PfCK1 and its appearance on the RBC membrane. We found t...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.2294
更新日期:2020-06-01 00:00:00
abstract::Calcium-sensing receptor (CaSR) contributes to maintain homeostatic levels of extracellular calcium. In addition, CaSR controls other cellular activities such as proliferation and migration, particularly in cells not related to extracellular calcium homeostasis, potentially by cross-talking with parallel signaling pat...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.1232
更新日期:2013-12-01 00:00:00
abstract::Since about 60 years a phenomenon now called permeability transition is known in mitochondria. It involves a large pore in the inner mitochondrial membrane, the permeability transition pore (PTP) whose molecular structure is still unknown. Year after year, new hypotheses have been developed how this pore could look li...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1002/iub.1130
更新日期:2013-03-01 00:00:00
abstract::The human nuclear estrogen receptors (i.e., ERalpha and ERbeta), as other transcriptional factors, regulate cellular processes inducing genomic events. In addition, the binding of 17beta-estradiol to ERs induces different membrane initiating non-genomic signaling. The non-genomic effects of ER are independent of trans...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/1521654031000081256
更新日期:2003-01-01 00:00:00
abstract::We propose a new hypothesis for the molecular mechanism by which neuroglobin exerts its protective effect in hypoxia-induced cell death. Our recent observation of a very rapid electron-transfer reaction between ferrous neuroglobin and ferric cytochrome c is central to this hypothesis. In contrast to previously suggest...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.35
更新日期:2008-06-01 00:00:00
abstract::Over the past decade, following the discovery of the human heme protein neuroglobin, many studies have searched for evidence for this protein's mechanism of action. Much data has accrued showing that high levels of neuroglobin will protect cells from apoptotic cell death, following a wide range of challenges. Various ...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1002/iub.405
更新日期:2010-12-01 00:00:00
abstract::To further understand the role of the three conserved Val residues in insulin, B12Val, B18Val, and A3Val, five insulin mutants-[A3Ser]insulin, [B12Thr]insulin, (desB30)[B12Ser]insulin, [B18Thr] insulin, and [B18Leu]insulin--were obtained by means of site-directed mutagenesis and their receptor-binding activities as we...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/152165401317291165
更新日期:2001-12-01 00:00:00
abstract::We hypothesize that the various, previously reported, reactivities of neuroglobin with redox partners and oxygen provide for the establishment of a redox cycle within cells, such as neurons and retinal rod cells. Using native cell lysates, from cultured human cells of neuronal origin, we have estimated the rate of red...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.566
更新日期:2012-05-01 00:00:00
abstract::The sodA gene coding for manganese superoxide dismutase (Mn-SOD) from the marine microorganism Vibrio parahaemolyticus was cloned, sequenced, and overexpressed in Escherichia coli by use of the pET20b (+) expression vector. The full-length gene consisted of a 588-bp open reading frame and encoded a polypeptide of 196 ...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/713803521
更新日期:1999-09-01 00:00:00
abstract::The effect of streptozotocin-induced diabetes on cholinesterases activities was studied in the retina and, for comparison, in other nervous and nonnervous tissues. Streptozotocin diabetes did not affect acetylcholinesterase activity in the retina but increased its activity in the cerebral cortex (100%) and in serum (5...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/15216540050033140
更新日期:2000-04-01 00:00:00
abstract::Although the amino acid sequences and the structures of pyruvate kinase (PYK) isozymes are highly conserved, allosteric regulations differ. This suggests that amino acids with low conservation play important roles in the allosteric mechanism. The current work exploits a 'natural screen'- the 122 point mutations identi...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/15216540500531705
更新日期:2006-01-01 00:00:00
abstract::Heparin and its various derivatives affect cancer progression in humans. In this study, we show that heparin uptaken intracellularly by melanoma cells activated a signaling cascade, which in turn inhibited melanoma cell adhesion and migration. The reduced ability of M5 cells to adhere onto the fibronectin (FN) substra...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.421
更新日期:2011-02-01 00:00:00
abstract::Recent structural studies of proteins mediating membrane fusion reveal intriguing similarities between diverse viral and mammalian systems. Particularly striking is the close similarity between the transmembrane envelope glycoproteins from the retrovirus HTLV-1 and the filovirus Ebola. These similarities suggest simil...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/713803503
更新日期:1999-08-01 00:00:00
abstract::Mutations in the breast cancer susceptibility gene BRCA1 predispose individuals to breast and ovarian cancers. Cofactor of BRCA1 (COBRA1), a novel protein, was isolated as a BRCA1-interacting protein. However, the role of COBRA1 in breast cancer is poorly understood. In this study, we demonstrate that COBRA1 mRNA was ...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/15216540410001699826
更新日期:2004-03-01 00:00:00
abstract::Cartilage engineering is one challenging issue in regenerative medicine. Low oxygen tension or hypoxia inducible factor-1 (HIF-1α) gene therapy are promising strategies in the field of cartilage repair. Previously, we showed that hypoxia and its mediator HIF-1 regulate matrix genes expression (collagens and aggrecan)....
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.1539
更新日期:2016-09-01 00:00:00
abstract::Although vitamin E has been recognized as a critical micronutrient to neuronal health for more than half a century, vitamin E transport and regulation in the brain remain a mystery. Currently, the majority of what is known about vitamin E transport has been delineated in the liver. However, clues from the pathogenesis...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1002/iub.1993
更新日期:2019-04-01 00:00:00
abstract::Recent successes in identifying the genes and associated proteins underlying several familial neurodegenerative conditions have not always resulted in accounts as to why the associated patterns of neuronal damage are so specific and limited. Here, with reference to Huntington's disease, we present a general scheme to ...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/1521654031000153012
更新日期:2003-06-01 00:00:00
abstract::Calcineurin (CN) is the only serine/threonine specific protein phosphatase regulated by Ca(2+) /calmodulin (CaM), which is composed of catalytic A subunit (CNA) and regulatory B subunit (CNB). Tumor necrosis factor (TNF) receptor associated factor 3 (TRAF3) is an essential component in the Toll like receptors and TNF ...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.1060
更新日期:2012-09-01 00:00:00
abstract::Our results demonstrate that open reading frame (ORF) YHR076w on chromosome VIII of Saccharomyces cerevisiae that was previously described as a hypothetical gene is expressed. This ORF is transcribed as an mRNA of approximately 1,100 nucleotides. A 41.2-kDa polypeptide and three others predicted to be modified forms o...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/713803741
更新日期:2000-12-01 00:00:00
abstract::N-Acetyl-seryl-aspartyl-lysyl-proline (Ac-SDKP) is a critical negative regulator of fibrosis development in the liver. However, its extremely short half-life in vivo greatly compromises its potential applications. Here, we report an Ac-SDKP analog peptide with d-amino acid replacement (Ac-SDD KD P). The stability of A...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.2037
更新日期:2019-09-01 00:00:00
abstract::Pilins or fimbrilins are a class of proteins found in bacterial surface pilus, a hair-like surface appendage. Both the Gram-negative and -positive bacteria produce pilins to assemble pili on their cell-surface for different purposes including adherence, twitching motility, conjugation, immunomodulation, biofilm format...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1002/iub.1400
更新日期:2015-07-01 00:00:00
abstract::The posttranscriptional regulation of miRNAs is important for organism development. To investigate the role of miRNAs in angiogenesis, we performed a loss-of-function screening assay in human umbilical vein endothelial cells (HUVECs) and found that knockdown of 7 miRNAs (miR-95a, miR-126, miR-129, miR-137, miR-139, mi...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.486
更新日期:2011-07-01 00:00:00
abstract::Occlusion of a coronary artery if maintained for longer period of time results in damage of the cardiac tissue. However, restoration of blood flow to previously ischemic tissue can itself induce further cardiac damage, a phenomenon known as myocardial reperfusion injury. Cardiac homoeostasis is supported by a network ...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1002/iub.1352
更新日期:2015-02-01 00:00:00
abstract::Diarrhea-predominant irritable bowel syndrome (IBS-D) is one of the most common gastrointestinal disorders in the world, lacking effective therapies. The crucial roles of microRNAs (miRNAs) in IBS-D have attracted increasing attention. The aim of this study is to investigate the effects of miR-495 on the visceral sens...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.2270
更新日期:2020-07-01 00:00:00
abstract::Hepatitis C virus (HCV) is an enveloped positive-strand RNA virus of the Flaviviridae family. It has a genome of about 9,600 nucleotides encoding a large polyprotein (about 3,000 amino acids) that is processed by cellular and viral proteases into at least 10 structural and nonstructural viral proteins. A novel HCV pro...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1002/iub.201
更新日期:2009-07-01 00:00:00
abstract::In protein and RNA macromolecules, only a limited number of different side-chain chemical groups are available to function as catalysts. The myriad of enzyme-catalyzed reactions results from the ability of most of these groups to function either as nucleophilic, electrophilic, or general acid-base catalysts, and the k...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/15216540211468
更新日期:2002-02-01 00:00:00
abstract::Recently, cytochrome P450 170A1 (CYP170A1) has been found to be a bifunctional protein, which catalyzes both monooxygenase activity and terpene synthase activity by two distinct active sites in the well-established P450 protein structure. Therefore, CYP170A1 is identified clearly as a moonlighting protein. The known a...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1002/iub.501
更新日期:2011-07-01 00:00:00
abstract::The activation and incorporation of selenium into selenocysteine containing selenoproteins has been well established in an Escherichia coli model system but there is little specific information concerning the transport and intracellular trafficking of selenium in biological systems in general. A selenium transport rol...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/15216540400010818
更新日期:2004-08-01 00:00:00
abstract::Selenium is an essential component of several enzymes and proteins in a number of methane-producing archae. Information concerning accessory proteins that function in selenium transport processes, however, is limited. A novel selenium-binding protein with a potential transport role and a selenocysteine lyase that serv...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/15216540400008911
更新日期:2004-07-01 00:00:00