Abstract:
:The clown anemonefish (Amphiprion ocellaris) is a common model species in studies assessing the impact of climate changes on tropical coral fish physiology, metabolism, growth, and stress. However, the basic endocrine principles for the control of food intake and energy homeostasis, under normal and elevated sea temperatures, in this species remain unknown. In this work, we studied food intake and growth in clown anemonefish reared at different temperatures and with different food availability. We also analyzed expression of genes in the melanocortin system, which is believed to be involved in the control of appetite and feeding behavior. These were two paralogues of pomc: pomca and pomcb; two paralogs of agrp: agrp1 and agrp2; and one mc4r-like. Groups of juvenile clown anemonefish were exposed to four experimental treatments combining (orthogonal design) two rearing temperatures: 28 °C (T28; normal) and 32 °C (T32; high) and two feeding regimes: one (1 M; 08:00) or three (3 M; 08:00, 12:00, 15:00) meals per day, fed to satiety by hand. The results showed that high temperature (T32) did not affect the average growth rate but induced a stronger asymmetrical individual body weight of the fish within the population (tank). Lower feeding frequency (1 M) resulted in lower growth rates at both rearing temperatures. Fish reared at high temperature had higher total daily food intake, which correlated with a lower expression of pomca, supporting an anorexigenic role of this gene. High temperature combined with restricted feeding induced higher agrp1 levels and resulted in a higher food intake in the morning meal compared to the control. This supports an orexigenic role for agrp1. mRNA levels of agrp2 responded differently from agrp1, supporting different roles for the paralogues. Levels of mc4r-like inversely correlated with fish body weight, indicating a possible size/stage dependence of gene expression. In conclusion, our results indicate that the melanocortin system is involved in adjusting appetite and food intake of clown anemonefish in response to elevated temperature and low food availability.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Pham LP,Olderbakk Jordal AE,Nguyen MV,Rønnestad Idoi
10.1016/j.ygcen.2021.113719subject
Has Abstractpub_date
2021-01-18 00:00:00pages
113719eissn
0016-6480issn
1095-6840pii
S0016-6480(21)00011-3pub_type
杂志文章abstract::This study was designed to examine the relationship among ovulation rate, inhibin alpha-subunit gene expression, and plasma immunoreactive inhibin levels between hens laying at a high ovulation rate and hens laying at a low ovulation rate. A porcine inhibin alpha-subunit cDNA was used to examine the expression of inhi...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1993.1103
更新日期:1993-07-01 00:00:00
abstract::Urinary corticosterone metabolite responses to capture have recently been shown for the first time in amphibians, and in the present study urinary corticosterone metabolite responses to capture and to confinement in captivity were measured in adult cane toads (Rhinella marina) in Queensland, Australia. An adrenocortic...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2011.06.015
更新日期:2011-09-01 00:00:00
abstract::Extrapituitary expression of the growth hormone (GH) gene has been reported for the immune system of various vertebrates. In the rainbow trout (Oncorhynchus mykiss), GH mRNA could be detected in several lymphoid organs and leucocytes by reverse transcriptase-polymerase chain reaction (RT-PCR). To understand the contro...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2005.01.008
更新日期:2005-05-15 00:00:00
abstract::Chronic confinement for 1 month caused a significant elevation of plasma cortisol but suppressed the levels of plasma testosterone and 11-ketotestosterone in sexually mature male brown trout. An acute handling stress for 1 hr elevated blood cortisol and ACTH levels and also suppressed circulating androgens. This andro...
journal_title:General and comparative endocrinology
pub_type: 杂志文章,评审
doi:10.1016/0016-6480(87)90036-0
更新日期:1987-11-01 00:00:00
abstract::The growth hormone (Gh)/insulin-like growth-factor (Igf) system plays a central role in the regulation of growth in fishes. However, the roles of Igf binding proteins (Igfbps) in coordinating responses to food availability are unresolved, especially in anadromous fishes preparing for seaward migration. We assayed plas...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2016.05.015
更新日期:2016-07-01 00:00:00
abstract::The binding of 125I-labeled ovine prolactin (oPRL) to membrane preparations of tissue from freshwater-adapted tilapia (Sarotherodon mossambicus) was examined. Liver, ovary, and testis showed a relatively high specific binding (5-10%). A lower specific binding occurred consistently in intestine and gill tissue, and inc...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(84)90056-x
更新日期:1984-10-01 00:00:00
abstract::The effects of starvation and feeding on the release of bombyxin, a peptide of insulin superfamily in insects, from the larval brain of the silkworm Bombyx mori were investigated. Following starvation, the bombyxin titer in the hemolymph of larvae decreased, whereas its content in the brain increased. On the other han...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1999.7438
更新日期:2000-06-01 00:00:00
abstract::Secretin (Sct), traditionally a gastrointestinal hormone backed by a century long research, is now beginning to be recognized also as a neuroactive peptide. Substantiation by recent evidence on the functional role of Sct in various regions of the brain, especially on its potential neurosecretion from the posterior pit...
journal_title:General and comparative endocrinology
pub_type: 杂志文章,评审
doi:10.1016/j.ygcen.2012.11.017
更新日期:2013-01-15 00:00:00
abstract::Immunochemical investigations, using dot immunobinding assay (DIA) and enzyme-linked immunosorbent assay (ELISA), and immunocytochemical studies reveal the following new information about crustacean vitellogenesis-inhibiting hormone (VIH): (1) The structure of VIH is sufficiently different from that of the other sinus...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(87)90187-0
更新日期:1987-09-01 00:00:00
abstract::Three distinct thyroid hormone receptor (TR) cDNAs (gTRalphaA, gTRalphaB and gTRbeta) were cloned and sequenced in orange-spotted grouper (Epinephelus coioides). The cDNA of gTRalphaA consisted of 1555-bp and encoded a putative protein of 416 amino acids, while the cDNA of gTRalphaB consisted of 1459-bp and encoded 39...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2008.08.015
更新日期:2008-11-01 00:00:00
abstract::Living for extended periods in Antarctica exposes base personnel to extremes of daylength (photoperiod) and temperature. At the British Antarctic Survey base of Halley, 75°S, the sun does not rise for 110 d in the winter and does not set for 100 d in summer. Photoperiod is the major time cue governing the timing of se...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2017.05.010
更新日期:2018-03-01 00:00:00
abstract::The aim of this study was to identify the major C21 steroids produced in vivo during artificially induced final oocyte maturation and spawning in female common dentex (Dentex dentex). During the spawning season, mature females were treated with a gonadotropin-releasing hormone agonist (GnRHa)-loaded delivery system, w...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.2000.7519
更新日期:2001-07-01 00:00:00
abstract::Previous reports have shown that adenosine in rat inhibits both spontaneous and ACTH-induced release of corticosteroids through activation of adenosine A1 receptors. In the present study, we have investigated the possible effect of adenosine in the secretion of corticosteroids in amphibians using a perfusion technique...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(92)90070-z
更新日期:1992-06-01 00:00:00
abstract::Urinary excretion of the thyroid hormones (TH) L-thyroxine (T4), and 3,5,3'-triiodo-L-thyronine (T3) and their derivatives was studied in urinary-catheterized rainbow trout (mean wt. 227 g) fasted for 2 days under a 12-hr L:12-hr D photocycle in running water (12 degrees). Catheterized trout were intracardiac-injected...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1994.1128
更新日期:1994-08-01 00:00:00
abstract::In fish, luteinizing hormone (LH) stimulates processes leading to final oocyte maturation and ovulation in females, and spermiation in males. The hormone is a heterodimeric glycoprotein composed of two non-covalently associated subunits. In this study, we describe the expression of tilapia LH (tLH) as a biologically a...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2004.10.016
更新日期:2005-02-01 00:00:00
abstract::Plasma concentrations of stress hormones [adrenaline (ADR), noradrenaline (NR)], lactate, glucose and CO2 were monitored during peak nesting period (May-October) at different phases of nesting in the green turtle, Chelonia mydas. These include, emergence from sea, excavating body and nest chambers, oviposition, coveri...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2007.09.012
更新日期:2008-02-01 00:00:00
abstract::Somatolactin (SL) and SL receptor (SLR) belong to the growth hormone and cytokine type I receptor superfamilies, respectively. However, further research is required to define the duplications and functions of SL and its receptors in basal vertebrates including environmental background color adaptation in fish. In the ...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2011.12.023
更新日期:2012-03-01 00:00:00
abstract::Neuropeptides are ancient endocrine components which have evolved to regulate many aspects of biology across the animal kingdom including behaviour, development and metabolism. To supplement current knowledge, we have utilized a transcriptome series describing larval development in the ornate spiny lobster, Panulirus ...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2020.113496
更新日期:2020-08-01 00:00:00
abstract::The skin of Xenopus laevis, because of its high concentrations of amidated peptides, is an excellent model for the study of neuropeptide biosynthesis. In this paper we determined levels of the mRNAs which encode cerulin and characterized the enzymatic activity which likely produces the carboxyl terminal alpha-amide mo...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(87)90206-1
更新日期:1987-07-01 00:00:00
abstract::Myopathies (Woody Breast (WB) and White Striping (WS)) of broiler chickens have been correlated with fast growth. Recent studies reported that localized hypoxia and metabolic impairment may involve in these myopathies of birds. In order to better understand the stress response mechanisms affecting myopathies of broile...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2020.113526
更新日期:2020-09-01 00:00:00
abstract::Two forms of immunoreactive gonadotropin-releasing hormone (GnRH) were extracted from brain-pituitary tissues of Thai catfish, Clarias macrocephalus and C. batrachus. The peptides were detected using high performance liquid chromatography (HPLC) and radioimmunoassay (RIA). In both the HPLC systems, catfish GnRH-I elut...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(92)90031-e
更新日期:1992-08-01 00:00:00
abstract::High-resistance epithelia derived from amphibian sources such as frog skin, toad urinary bladder, and the A6 Xenopus laevis kidney cell line have been widely used to elucidate the underlying mechanisms involved in the regulation of vectorial ion transport. More recently, the isolation of high-resistance mammalian cell...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2005.11.010
更新日期:2006-05-15 00:00:00
abstract::We investigated the effect of several potential carbohydrate secretagogues, amino acids, a ketoacid, and potassium chloride on insulin, glucagon, and somatostatin release from the in vitro perfused Brockmann body of channel catfish (Ictalurus punctatus). Mannose (15 mM) stimulated the release of insulin and somatostat...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(87)90120-1
更新日期:1987-03-01 00:00:00
abstract::Vasotocin (VT) and vasopressin control many endocrine and neuroendocrine functions, including the regulation of reproductive behaviors. In the roughskin newt (Taricha granulosa), VT administration can enhance courtship behaviors in males and egg-laying behaviors in females. This study used immunohistochemistry to inve...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1999.7424
更新日期:2000-02-01 00:00:00
abstract::The zebrafish is a powerful genetic model organism. In recent years, zebrafish has been increasingly used to model human diseases. Due to a number of recent technological advancements, the genetic tool box is now also stocked with sophisticated transgenic and reverse genetic tools. Here, we focus on both commonly used...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2013.02.002
更新日期:2013-07-01 00:00:00
abstract::This study was conducted at the Bukhara breeding centre (Ouzbekistan, Central Asia) on Persian gazelles (Gazella subgutturosa subgutturosa) and at Thumammah (Saudi Arabia) on sand gazelles (Gazella subgutturosa marica). Plasma prolactin (PRL) and progesterone were determined in groups of females. Because these females...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.2000.7561
更新日期:2001-01-01 00:00:00
abstract::Several of the song control nuclei of songbirds, including HVc (higher vocal center) and Area X, contain melatonin receptor (MelR). In laboratory-housed male starlings, the densities of MelR in Area X change markedly according to reproductive state. MelR are down-regulated when starlings are photostimulated (in full b...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/s0016-6480(03)00261-2
更新日期:2003-11-01 00:00:00
abstract::The widely accepted paradigm that epoxidized methyl farnesoates ("juvenile hormones," JHs) are the principal sesquiterpenoid hormones regulating insect metamorphosis was assessed in Drosophila melanogaster. GC-MS analysis of circulating methyl farnesoids during the mid to late 3rd instar showed that methyl farnesoate ...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2012.11.009
更新日期:2013-02-01 00:00:00
abstract::The distribution of melatonin was studied at midday and midnight in pigeon plasma, pineal, hypothalamus, eyes, Harderian glands, and duodenum. Tissue samples were homogenized, extracted with chloroform, evaporated, and measured by our melatonin radioimmunoassay (RIA). All the extracts gave dilution curves parallel to ...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(85)90136-4
更新日期:1985-04-01 00:00:00
abstract::Migratory birds undergo metabolic remodeling in tissues, including increased lipid storage in white adipose and fatty acid uptake and oxidation in skeletal muscle, to optimize energy substrate availability and utilization in preparation for long-distance flight. Different tissues undergo gene expression changes in kee...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2018.08.009
更新日期:2018-11-01 00:00:00