Abstract：
:In fish, luteinizing hormone (LH) stimulates processes leading to final oocyte maturation and ovulation in females, and spermiation in males. The hormone is a heterodimeric glycoprotein composed of two non-covalently associated subunits. In this study, we describe the expression of tilapia LH (tLH) as a biologically active, single-chain polypeptide using the methylotrophic yeast Pichia pastoris. The tLHbeta and alpha mature protein-coding sequences were joined to form a fusion gene that encodes a "tethered" polypeptide in which the tLHbeta-chain forms the N-terminal part and the alpha-chain forms the C-terminal part. A "linker" sequence of six amino acids (three Gly-Ser pairs) was placed between the beta- and alpha-chains to assist in the chimerization of the subunits, and a six-His tail was placed at the end of the beta-subunit, to enable purification of the recombinant protein. Western blot analysis of the pituitary LH resolved by SDS-PAGE yielded a band of 35 kDa, while the recombinant tLHbetaalpha had a molecular mass of 45 kDa, and was found to possess only N-linked carbohydrates. Recombinant tLHbetaalpha stimulated the release of 11-ketotestosterone from mature testes, whereas its release from immature testes was less pronounced.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Kasuto H,Levavi-Sivan Bdoi
10.1016/j.ygcen.2004.10.016keywords:
subject
Has Abstractpub_date
2005-02-01 00:00:00pages
222-32issue
3eissn
0016-6480issn
1095-6840pii
S0016-6480(04)00288-6journal_volume
140pub_type
杂志文章abstract：:The distribution and properties of nonapeptide binding sites in the kidney of the anuran Xenopus laevis were investigated using quantitative in vitro autoradiography. The binding studies were performed with [3H]arginine vasopressin (AVP) as ligand because [125I]arginine vasotocin (AVT) lacks biological activity. Speci...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(92)90173-h
更新日期：1992-01-01 00:00:00
abstract：:Chicken growth hormone (cGH) has been shown to affect chicken skeletal muscle satellite cell proliferation and differentiation in vitro. This study describes the interactions of cGH with basic fibroblast growth factor (bFGF) and insulin-like growth factor I (IGF-I). Both cGH and bFGF induced cGH receptor (cGH-R) gene ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1997.6964
更新日期：1997-10-01 00:00:00
abstract：:To examine the production of steroids with potential oocyte maturation-inducing or pheromonal activity in the goldfish (Carassius auratus) we have incubated mature ovaries of this species with 17-[3H]hydroxyprogesterone. The metabolites in the unconjugated, glucuronide, and sulfate fractions were identified by chromat...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(92)90044-k
更新日期：1992-09-01 00:00:00
abstract：:In fish there are few data on the gonadotropin-releasing hormone (Gn-RH) neurosecretory activity, which could explain long- and short-term variations of the gonadotropin secretion. There is no biological species specificity between mammal and fish Gn-RH; although there is a structural difference, they are, on the cont...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(86)90255-8
更新日期：1986-01-01 00:00:00
abstract：:Progesterone is believed to have a suppressive or inhibitory role in regulating androgen-dependent sex behaviors in male mammals and birds. Previous studies in this laboratory have revealed that in the little striped whiptail lizard (Cnemidophorus inornatus), progesterone (P) can stimulate sex behavior in a proportion...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(92)90125-4
更新日期：1992-04-01 00:00:00
abstract：:Using morpholino antisense oligonucleotide (MO) technology, we blocked leptin A or leptin receptor expression in embryonic zebrafish, and analyzed consequences of leptin A knock-down on fish development. Embryos injected with leptin A or leptin receptor MOs (leptin A or leptin receptor morphants) had smaller bodies an...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2012.07.011
更新日期：2012-09-15 00:00:00
abstract：:The distribution of melatonin was studied at midday and midnight in pigeon plasma, pineal, hypothalamus, eyes, Harderian glands, and duodenum. Tissue samples were homogenized, extracted with chloroform, evaporated, and measured by our melatonin radioimmunoassay (RIA). All the extracts gave dilution curves parallel to ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(85)90136-4
更新日期：1985-04-01 00:00:00
abstract：:Testosterone was isolated and quantified from male lobster serum and testes by solvent extraction, sequential thin-layer chromatography, and high-performance liquid chromatography. The identity of the isolated steroid was established by its isopolarity and isomorphicity with authentic radiolabeled testosterone and its...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(84)90158-8
更新日期：1984-06-01 00:00:00
abstract：:Changes in the circulating levels of thyroid hormones measured by radioimmunoassay in yearling coho salmon in response to injection of bovine thyroid-stimulating hormone (TSH) were evaluated from January through August. Doses of TSH ranging from 0.2 to 6.4 micrograms/g body wt were ineffective in stimulating T4 in Jan...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(87)90087-6
更新日期：1987-12-01 00:00:00
abstract：:The golden rabbitfish Siganus guttatus is a reef fish with a restricted lunar-synchronized spawning rhythmicity and releases gametes simultaneously around the first quarter moon period during the spawning season. In order to understand the molecular aspects of the "circa" rhythms in this species, the full-length melat...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2006.08.011
更新日期：2007-01-15 00:00:00
abstract：:Growth hormone (GH) is a key factor controlling postnatal growth and development. Despite growth-promoting effects in mammals, GH is not associated with muscle growth in the chicken. Janus kinase 2 (JAK2) has been identified as the first intracellular step in GH receptor (GHR) signaling in many species, however, there...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2005.05.001
更新日期：2005-11-01 00:00:00
abstract：:The skin is the largest organ in the body and is a barrier between the internal and external environment. The present study evaluates how PTU, a goitrogen, that is used to treat hyperthyroidism affects the structure and electrical properties of the frog (Xenopus laevis) skin. The results are considered in the context ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2011.09.007
更新日期：2011-12-01 00:00:00
abstract：:Three types of cDNA encoding the arginine vasotocin (AVT) receptors from the newt, Cynops pyrrhogaster were cloned and the gene expression of each receptor analyzed in the organs and tissues of the newt. The deduced amino acid sequence of one type of AVT receptor, consisting of 418 amino acid residues, showed a high d...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2007.02.002
更新日期：2007-05-01 00:00:00
abstract：:The present study examined plasma concentrations of insulin-like growth factor (IGF)-I, IGF-II and IGF-binding proteins (IGFBPs) during posthatch growth and development in chickens. Three distinct proteins which bound 125I-IGF-I were observed irrespective of age or sex, these having apparent molecular weights of 22, 2...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1997.6898
更新日期：1997-07-01 00:00:00
abstract：:The present investigation was designed to (1) determine if atrial natriuretic factor gene expression occurs within invertebrates as well as within vertebrates; (2) determine whether the product of this gene expression is the 126-amino-acid atrial natriuretic factor prohormone or some other molecular species; and (3) e...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1995.1133
更新日期：1995-10-01 00:00:00
abstract：:The concentrations of norepinephrine in hypothalamus and norepinephrine and epinephrine in head kidney were significantly decreased in treated tilapia (Oreochromis aureus) during the time course of cold exposure (12 degrees) as compared to the control (25 degrees). The elevation of norepinephrine and epinephrine in pl...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.2001.7772
更新日期：2002-03-01 00:00:00
abstract：:The Hexapoda consists of two classes, the Entognatha and the Insecta, with the former group considered basal to the latter. The Protura is a basal order within the Entognatha, the members of which are minute soil dwellers first identified in the early 20th century. Recently, a transcriptome shotgun assembly (TSA) was ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2015.05.015
更新日期：2015-12-01 00:00:00
abstract：:Vasotocin (VT) and vasopressin control many endocrine and neuroendocrine functions, including the regulation of reproductive behaviors. In the roughskin newt (Taricha granulosa), VT administration can enhance courtship behaviors in males and egg-laying behaviors in females. This study used immunohistochemistry to inve...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1999.7424
更新日期：2000-02-01 00:00:00
abstract：:Neuropeptides are ancient endocrine components which have evolved to regulate many aspects of biology across the animal kingdom including behaviour, development and metabolism. To supplement current knowledge, we have utilized a transcriptome series describing larval development in the ornate spiny lobster, Panulirus ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2020.113496
更新日期：2020-08-01 00:00:00
abstract：:Growth hormone (GH) regulates essential physiological functions in teleost fishes, including growth, metabolism, and osmoregulation. Recent studies have identified two clades of putative receptors for GH (GHR1 clade and GHR2 clade) in fishes, both of which are highly expressed in the liver. Moreover, the liver is an i...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2012.07.010
更新日期：2012-10-01 00:00:00
abstract：:Application of alpha-MSH immunohistochemistry to the brain of elasmobranchs (Scyliorhinus torazame and Etmopterus brachyurus) demonstrated a marked species difference concerning the distribution of the alpha-MSH-like molecule in the brain. In S. torazame, alpha-MSH-like immunoreactive cells were present in the hypotha...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.2001.7628
更新日期：2001-06-01 00:00:00
abstract：:In altricial songbirds, food restriction in early development has adverse effects on various physiological systems. When conditions improve birds can accelerate growth, but this compensatory strategy is associated with long-term adverse consequences. One system affected by altered growth rates is the hypothalamic-pitu...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2015.08.001
更新日期：2015-10-01 00:00:00
abstract：:Bicuspid teeth are a morphological characteristic in adult metamorphosed urodeles. However, during the mating season the males of many species of plethodontid salamanders (Urodela, Plethodontidae) from northern America bear large monocuspid teeth on their jaws. Since androgen levels are elevated during the mating seas...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/s0016-6480(03)00216-8
更新日期：2003-10-15 00:00:00
abstract：:Effects of growth hormone (GH) on the synthesis of mucopolysaccharide by ceratobranchial cartilages of the Japanese eel, Anguilla japonica, were examined by monitoring the in vitro uptake of [35S]sulfate. The [35S]sulfate uptake decreased rapidly to one-third of the initial level during the first 3 days after hypophys...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(90)90117-5
更新日期：1990-08-01 00:00:00
abstract：:This review focuses on the endocrine regulation of reproductive behavior in moth species with particular emphasis on Helicoverpa spp. Reproductive behavior in most adult moths is dependent on the release of a unique blend of sex pheromones by the females to attract conspecific males. Mating, on the other hand, results...
journal_title：General and comparative endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.ygcen.2008.04.004
更新日期：2009-05-15 00:00:00
abstract：:To quantify the renal excretion of arginine vasotocin (AVT) in birds, synthetic AVT was infused into conscious Pekin ducks at rates of 0.03, 0.10, and 0.30 ng/kg/min for 30 min and the relationships between plasma and urine AVT concentrations were monitored by radioimmunoassay. As plasma AVT concentrations increased (...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1995.1090
更新日期：1995-07-01 00:00:00
abstract：:Melatonin is a much conserved feature in vertebrates that plays a central role in the entrainment of daily and annual physiological rhythms. Investigations aiming at understanding how melatonin mediates the effects of photoperiod on crucial functions and behaviors have been very active in the last decades, particularl...
journal_title：General and comparative endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.ygcen.2009.04.026
更新日期：2010-02-01 00:00:00
abstract：:The insulin-like growth factor-1 (IGF-1) plays an important role in the regulation of reproductive function. In the present study, we examined the effects of caloric restriction (CR) on the reproductive lifespan in rats and investigated the potential role of IGF-1. After 10 weeks of treatment, we determined the distri...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2011.09.005
更新日期：2011-11-01 00:00:00
abstract：:The activities of oxidative enzymes of hepatic mitochondria of Anabas testudineus increased following exogenous administration of hydrocortisone and corticosterone. The inhibitors of protein synthesis prevented the hormone-mediated increase of hepatic enzymes, suggesting that corticosteroids regulate the oxidative met...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(90)90017-g
更新日期：1990-05-01 00:00:00
abstract：:Previous studies have provided evidence that D-Asp plays a role in steroid-mediated reproductive biology in amphibians, reptiles, birds and mammals. To examine the molecular involvement of D-Asp on steroidogenic pathway regulation, we analysed the expression of StAR, P450 aromatase and 5αRed2 mRNAs in Pelophylax escul...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2011.10.006
更新日期：2012-01-01 00:00:00
