Abstract：
:Using morpholino antisense oligonucleotide (MO) technology, we blocked leptin A or leptin receptor expression in embryonic zebrafish, and analyzed consequences of leptin A knock-down on fish development. Embryos injected with leptin A or leptin receptor MOs (leptin A or leptin receptor morphants) had smaller bodies and eyes, undeveloped inner ear, enlarged pericardial cavity, curved body and/or tail and larger yolk compared to control embryos of the same stages. The defects persisted in 6-9 days old larvae. We found that blocking leptin A function had little effect on the development of early brain (1 day old), but differentiation of both the morphant dorsal brain and retinal cells was severely disrupted in older (2 days old) embryos. Despite the enlarged pericardial cavity, differentiation of cardiac cells appeared to be similar to control embryos. Formation of the morphants' inner ear is also severely disrupted, which corroborates existing reports of leptin receptor expression in inner ear of both zebrafish and mammals. Co-injection of leptin A MO and recombinant leptin results in partial rescue of the wild-type phenotype. Our results suggest that leptin A plays distinct roles in zebrafish development.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Liu Q,Dalman M,Chen Y,Akhter M,Brahmandam S,Patel Y,Lowe J,Thakkar M,Gregory AV,Phelps D,Riley C,Londraville RLdoi
10.1016/j.ygcen.2012.07.011subject
Has Abstractpub_date
2012-09-15 00:00:00pages
562-72issue
3eissn
0016-6480issn
1095-6840pii
S0016-6480(12)00285-7journal_volume
178pub_type
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pub_type： 杂志文章
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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journal_title：General and comparative endocrinology
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journal_title：General and comparative endocrinology
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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abstract：:Recently, we demonstrated high serum leptin and 25(OH)D (calcidiol) in obese animals, with high C/EBPβ and PPARγ expression in adipose tissue. Since the role of vitamin D in adipogenesis remains controversial and hyperleptinemia is found in obesity, we asked if leptin could interfere in vitamin D action on adipocytes....
journal_title：General and comparative endocrinology
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