Localization of crustacean hyperglycemic and vitellogenesis-inhibiting hormones in separate cell types in the protocerebrum of the woodlouse Armadillidium vulgare (Crustacea, Isopoda).

Abstract:

:We gave an accurate immunolocalization of CHH (crustacean hyperglycemic hormone) and VIH (vitellogenesis-inhibiting hormone) in the brain and the sinus gland of the woodlouse Armadillidium vulgare. The two immune sera have been respectively raised against HPLC-purified CHH and against a small peptide derived from the N-terminus of VIH. By immunocytochemistry, we showed that CHH and VIH were synthesized in different perikarya and stored in different axon endings of the sinus gland. As in other crustacean species studied to date, CHH was located in the axon endings filled with the biggest granules. Immunoblotting confirmed that VIH was stored in the sinus glands of both the female and the male. These clear localizations of CHH- and VIH-antigens do not preclude that only one peptide is released from a given type of SG endings and do not rule out that each peptide can be involved in the control of different physiological processes.

journal_name

Gen Comp Endocrinol

authors

Azzouna A,Philippe M,Jarry T,Grève P,Martin G

doi

10.1016/s0016-6480(02)00645-7

keywords:

subject

Has Abstract

pub_date

2003-04-01 00:00:00

pages

134-42

issue

2

eissn

0016-6480

issn

1095-6840

pii

S0016648002006457

journal_volume

131

pub_type

杂志文章