Abstract:
:We gave an accurate immunolocalization of CHH (crustacean hyperglycemic hormone) and VIH (vitellogenesis-inhibiting hormone) in the brain and the sinus gland of the woodlouse Armadillidium vulgare. The two immune sera have been respectively raised against HPLC-purified CHH and against a small peptide derived from the N-terminus of VIH. By immunocytochemistry, we showed that CHH and VIH were synthesized in different perikarya and stored in different axon endings of the sinus gland. As in other crustacean species studied to date, CHH was located in the axon endings filled with the biggest granules. Immunoblotting confirmed that VIH was stored in the sinus glands of both the female and the male. These clear localizations of CHH- and VIH-antigens do not preclude that only one peptide is released from a given type of SG endings and do not rule out that each peptide can be involved in the control of different physiological processes.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Azzouna A,Philippe M,Jarry T,Grève P,Martin Gdoi
10.1016/s0016-6480(02)00645-7keywords:
subject
Has Abstractpub_date
2003-04-01 00:00:00pages
134-42issue
2eissn
0016-6480issn
1095-6840pii
S0016648002006457journal_volume
131pub_type
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