Isolation and identification of testosterone from the serum and testes of the American lobster (Homarus americanus).

Abstract:

:Testosterone was isolated and quantified from male lobster serum and testes by solvent extraction, sequential thin-layer chromatography, and high-performance liquid chromatography. The identity of the isolated steroid was established by its isopolarity and isomorphicity with authentic radiolabeled testosterone and its acetate derivative. The concentrations of testosterone were determined by high-performance liquid chromatography, by a double-isotope derivative assay, and by radioimmunoassay. The concentrations of testosterone as determined by the three methods were the same and were 0.3 ng/ml and 14.3 ng/g in lobster serum and testes, respectively.

journal_name

Gen Comp Endocrinol

authors

Burns BG,Sangalang GB,Freeman HC,McMenemy M

doi

10.1016/0016-6480(84)90158-8

subject

Has Abstract

pub_date

1984-06-01 00:00:00

pages

429-32

issue

3

eissn

0016-6480

issn

1095-6840

journal_volume

54

pub_type

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