Abstract:
:Sex steroid hormones have been widely detected in molluscs, and experiments have shown the importance of sex steroids in sex determination, gonadal tissue maturation and gametogenesis. Nevertheless, the signaling pathways of sex steroids in invertebrates have not yet been elucidated. In order to gain insights into the mechanism of sex steroid signaling in molluscs, we have, therefore, tried to isolate molluscan estrogen receptors from the prosobranch mollusc Thais clavigera. Cerebral ganglia of T. clavigera (Mollusca, Gastropoda, Prosobranchia) were subjected to RNA extraction, and degenerate primers for amino acid sequences conserved in vertebrate estrogen receptors were designed. PCR amplification using cerebral RNA and degenerate primers followed by 5'- and 3'-RACE identified the cDNA encoding T. clavigera estrogen receptor 1 (tcER1). The deduced amino acid sequence showed 93% identity in the DNA-binding domain and 72% identity in the ligand binding domain when compared to Aplysia estrogen receptor. Reporter gene assay revealed that tcER1 is constitutively active and unresponsive to estrogen. Quantitative analysis of the tcER1 mRNA level demonstrated the preferential expression in the ovary. Furthermore, cerebral ganglia expressed tcER1 at a high level in the spring followed by subsequent enlargement of the ovary in later seasons. These results suggest importance of tcER1 in the seasonal development of reproductive organs in T. clavigera.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Kajiwara M,Kuraku S,Kurokawa T,Kato K,Toda S,Hirose H,Takahashi S,Shibata Y,Iguchi T,Matsumoto T,Miyata T,Miura T,Takahashi Ydoi
10.1016/j.ygcen.2006.03.016subject
Has Abstractpub_date
2006-09-15 00:00:00pages
315-26issue
3eissn
0016-6480issn
1095-6840pii
S0016-6480(06)00104-3journal_volume
148pub_type
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