Characterization of a sea bream (Sparus aurata) thyroid hormone receptor-beta clone expressed during embryonic and larval development.

Abstract:

:A clone encoding thyroid hormone receptor-beta (TR-beta) was isolated from a sea bream (Sparus aurata) ovary cDNA library. Sea bream (sb)TR-beta is closely related to its counterparts from other vertebrates and, like them, preferentially binds T3 rather than T4. However, the putative sbTR-beta protein contains a nine-amino-acid insert that is also present in the corresponding proteins from flounder and salmon but absent in TR-betas from zebrafish and terrestrial vertebrates. Semiquantitative RT-PCR analysis showed that sbTR-beta transcripts begin to accumulate during gastrulation and increase markedly in quantity up to the period around hatch (ca. 40 h postfertilization) before declining slightly. In adult tissues, TR-beta mRNA was present in approximately equal quantities in heart, intestine, brain, kidney, skeletal muscle, liver, and gill. The significance of the relatively strong expression of TR-beta during sea bream embryogenesis is discussed.

journal_name

Gen Comp Endocrinol

authors

Nowell MA,Power DM,Canario AV,Llewellyn L,Sweeney GE

doi

10.1006/gcen.2001.7649

keywords:

subject

Has Abstract

pub_date

2001-07-01 00:00:00

pages

80-9

issue

1

eissn

0016-6480

issn

1095-6840

pii

S0016-6480(01)97649-X

journal_volume

123

pub_type

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