Abstract:
:A rapid and sensitive heterologous enzyme-linked immunosorbent assay (ELISA) was developed to measure rainbow trout maturational gonadotropin. Purified salmon maturational gonadotropin (sGtH II) was used as reference hormone. Optimization of the procedure was performed by using an anti-beta sGtH serum. Two procedures were developed: an equilibrium assay (which did not involve a preincubation step) which lasted for 8 hr and a nonequilibrium assay (which involved a preincubation step) which lasted for 26 hr. The nonequilibrium assay gave the best sensitivity (70 pg/ml sample). GtH II measurements on in vivo and in vitro samples from GnRH analogs or sGnRH experiments showed that the ELISA procedure could be used over a wide range of concentrations. The method was validated by comparing GtH II concentrations measured by both RIA and ELISA.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Salbert G,Bailhache T,Zohar Y,Breton B,Jego Pdoi
10.1016/0016-6480(90)90052-nsubject
Has Abstractpub_date
1990-04-01 00:00:00pages
110-22issue
1eissn
0016-6480issn
1095-6840pii
0016-6480(90)90052-Njournal_volume
78pub_type
杂志文章abstract::Growth hormone (GH) and insulin-like growth factor-I (IGF-I) play major roles in the endocrine regulation of fish growth, but their interdependency and mode of action has not been well elucidated. The GH-IGF-I system is essential for normal vertebral growth in mouse, but this has not been studied in fish. To study the...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2004.12.005
更新日期:2005-05-15 00:00:00
abstract::The maternal control directing the very first hours of life is of pivotal importance for ensuring proper development to the growing embryo. Thanks to the finely regulated inheritance of maternal factors including mRNAs and proteins produced during oogenesis and stored into the mature oocyte, the embryo is sustained th...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2016.03.028
更新日期:2017-05-01 00:00:00
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journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2011.09.005
更新日期:2011-11-01 00:00:00
abstract::The penultimate instar larvae of Mamestra brassicae have one ecdysteroid peak 2 days after ecdysis which induces larval ecdysis. Allatectomy deleted this peak and caused a larger peak 7 days later just before precocious pupation, in a pattern similar to the ecdysteroid titer of the last instar. Application of juvenile...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(86)90157-7
更新日期:1986-08-01 00:00:00
abstract::The pituitary is a central organ of the neuro-endocrine system in fish that plays critical roles in various physiological processes, including stress response and behavior. Although it is known that pituitary hormones can have a direct or indirect influence stimulating or suppressing the immune responses, whether ther...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2019.02.011
更新日期:2019-05-01 00:00:00
abstract::Measuring hair cortisol has attracted interest as a long term parameter for chronic stress evaluation. However, some studies support the hypothesis that locally produced cortisol, originating from the hair follicle or skin cells, affects concentrations in the hair. In an animal model the influence of different treatme...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2016.05.005
更新日期:2016-07-01 00:00:00
abstract::Recently, zebrafish has quickly risen as a model species for functional analysis of the brain-pituitary-gonad axis. However, one of the hurdles for such work in this popular model organism is the small size of its pituitary gland, which makes it difficult to investigate the regulation of pituitary hormone expression a...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2008.11.020
更新日期:2009-01-15 00:00:00
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journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2020.113556
更新日期:2020-11-01 00:00:00
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journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(86)90160-7
更新日期:1986-08-01 00:00:00
abstract::Current views on Agnathan phylogeny favor the hypothesis that the genera of holarctic lampreys belong to a single family (Petromyzontidae) and form an interrelated progression in which Petromyzon is near to Ichthomyzon at the base of the phylogenetic tree and Lampetra is the most derived. A stock similar to that of co...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1998.7203
更新日期:1999-02-01 00:00:00
abstract::Atlantic salmon (Salmo salar) broodstock were transferred from natural (12-16 degrees C) to controlled temperatures of 14, 18 or 22 degrees C for 3 months during vitellogenesis. Fertility and survival were significantly reduced in eggs from broodstock held at 22 degrees C relative to 14 or 18 degrees C. Endocrine mech...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2003.11.004
更新日期:2004-02-01 00:00:00
abstract::We have examined the distribution and physiological effects of three evolutionarily and sequence-related neuropeptides in Rhodnius prolixus. These neuropeptides, adipokinetic hormone (RhoprAKH), corazonin (CRZ) and adipokinetic hormone/corazonin-related peptide (RhoprACP) are present in distinct, non-overlapping neuro...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2013.10.012
更新日期:2014-01-01 00:00:00
abstract::This study aimed at investigating the role of sexual steroids in the regulation of the expression of the single aromatase gene and steroid receptor subtypes in the brain-pituitary-ovarian axis of the Japanese eel. Unlike other teleosts, which possess duplicated genes for aromatase, cyp19a1a and cyp19a1b, expressed in ...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2011.11.005
更新日期:2012-01-01 00:00:00
abstract::Corticosterone, progesterone, estradiol-17 beta, and estrone were quantified in plasma collected weekly (April-September) from renesting and nonlaying female American kestrels (Falco sparverius) paired with males in captivity. Hormone levels and body weights for laying females were maximal during courtship and egg-lay...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(86)90048-1
更新日期:1986-06-01 00:00:00
abstract::Serotonin (5-HT) is well known for modulating the release of GnRH and gonadotropin in teleosts. Reports on increased female:male ratio after the blockade of 5-HT biosynthesis proposed a role for 5-HT in brain sex differentiation. Two types of tryptophan hydroxylase (Tph), rate-limiting enzyme in the biosynthesis of 5-...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2009.11.009
更新日期:2010-04-01 00:00:00
abstract::Almost all physiological processes within the organism, including immune parameters and hormones, follow a circadian rhythm. These daily fluctuations are often observed in free-living organisms; however, little is known regarding hormonal and immune daily variations in anurans, particularly under laboratory conditions...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2020.113702
更新日期:2020-12-20 00:00:00
abstract::Circannual and circadian variations in plasma levels of steroids were estimated by radioimmunoassay in the female and male catfish, Heteropneustes fossilis, over two consecutive annual reproductive cycles. In the female catfish, testosterone (T), estradiol-17 beta (E2), and estrone (E1) were detectable in the plasma o...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(83)90221-6
更新日期:1983-05-01 00:00:00
abstract::Ghrelin, a peptide hormone produced by the stomach in mammals, stimulates growth hormone release and food intake. Recently, ghrelin was identified and characterized in chicken proventriculus and shown to stimulate growth hormone release but inhibit feed intake. The purpose of this work was to identify and further char...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2005.09.013
更新日期:2006-02-01 00:00:00
abstract::In order to delineate further the molecular evolution of the renin-angiotensin system in vertebrates, angiotensin I (ANG I) has been isolated after incubation of plasma and kidney extracts of emu (Dromiceus novaehollandiae), axolotl (Ambystoma mexicanum), and sea lamprey (Petromyzon marinus). The identified sequences ...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2003.10.011
更新日期:2004-02-01 00:00:00
abstract::Maintenance of cellular homeostasis influences ageing and it is determined by several factors, including efficient proteolysis of damaged proteins. The ubiquitin-proteasome system is the major protein degradation pathway in the cell. Specifically, the proteasome is responsible for clearance of abnormal, denatured or i...
journal_title:General and comparative endocrinology
pub_type: 杂志文章,评审
doi:10.1016/j.ygcen.2011.02.005
更新日期:2011-05-15 00:00:00
abstract::Effects of growth hormone (GH) on the synthesis of mucopolysaccharide by ceratobranchial cartilages of the Japanese eel, Anguilla japonica, were examined by monitoring the in vitro uptake of [35S]sulfate. The [35S]sulfate uptake decreased rapidly to one-third of the initial level during the first 3 days after hypophys...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(90)90117-5
更新日期:1990-08-01 00:00:00
abstract::Homogenates of maturing rainbow trout testes show specific binding sites for 125J-labeled hCG (= 125I-labeled hCG). The binding is competitively inhibited by unlabeled hCG and by a hypophyseal extract of rainbow trout. It could be demonstrated that the tissue 125J-hCG binding specificity is restricted to the gonadal p...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(83)90143-0
更新日期:1983-02-01 00:00:00
abstract::Big dynorphin (prodynorphin 209-240), dynorphin A (prodynorphin 209-225), dynorphin B (prodynorphin 228-240), beta-endorphin (beta-lipotrophin 61-90), or Met-enkephalin, each infused into the third ventricle, were tested for their effect on PRL release in the anesthetized turkey hen. Laying hens that received big dyno...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1993.1121
更新日期:1993-08-01 00:00:00
abstract::Quantification of glucocorticoid metabolites in feces has been shown to be a powerful tool in evaluating well-being in vertebrates. Little is known however about the hypothalamic-pituitary-adrenal axis response to stressors, and consequent glucocorticoid excretion, in reptiles. In a longitudinal study, fecal corticost...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2012.02.017
更新日期:2012-05-15 00:00:00
abstract::A detailed study was made of sex differentiation in three anteroposteriorly distributed body regions of the long gonadal fold in young Pacific hagfish (Eptatretus stouti) from 12.4 to 45 cm in length. In general, the posterior region, adjacent to the cloaca, remained undifferentiated, and only in specimens more than 2...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(90)90315-d
更新日期:1990-02-01 00:00:00
abstract::The vertebrate oxytocin and vasopressin receptors form a family of G-protein-coupled receptors (GPCRs) that mediate a large variety of functions, including social behavior and the regulation of blood pressure, water balance and reproduction. In mammals four family members have been identified, three of which respond t...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2011.10.011
更新日期:2012-01-01 00:00:00
abstract::Although elevated plasma cortisol levels and a reduction in food intake are common features of the response to stress in fish, the potential role of cortisol in the regulation of food intake in these animals is poorly understood. In this study, goldfish (Carassius auratus) were fed ad libitum for 21 days diets prepare...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2003.09.016
更新日期:2004-01-15 00:00:00
abstract::The effects of PGE1, PGE2, PGF2 alpha (2 micrograms/ml), and forskolin (10 microM) on follicle contraction of brook trout (salvelinus fontinalis) were studied by measuring the weight loss of punctured follicles during in vitro incubation. PGE1, PGE2, and forskolin significantly inhibited spontaneous contraction of fol...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(92)90238-f
更新日期:1992-12-01 00:00:00
abstract::The Hexapoda consists of two classes, the Entognatha and the Insecta, with the former group considered basal to the latter. The Protura is a basal order within the Entognatha, the members of which are minute soil dwellers first identified in the early 20th century. Recently, a transcriptome shotgun assembly (TSA) was ...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2015.05.015
更新日期:2015-12-01 00:00:00
abstract::The crab-eating frog Fejervarya cancrivora inhabits mangrove swamps and marshes in Southeast Asia. In the present study, circulating angiotensin II (Ang II), aldosterone (Aldo), arginine vasotocin (AVT), and corticosterone (Cort) concentrations as well as various blood parameters were studied under osmotically stressf...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2013.10.013
更新日期:2014-01-01 00:00:00