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Binding of 125I-hCG to rainbow trout (Salmo gairdneri) testis in vitro.

Abstract：

:Homogenates of maturing rainbow trout testes show specific binding sites for 125J-labeled hCG (= 125I-labeled hCG). The binding is competitively inhibited by unlabeled hCG and by a hypophyseal extract of rainbow trout. It could be demonstrated that the tissue 125J-hCG binding specificity is restricted to the gonadal preparation. The trout testis was characterized by determining affinity and capacity from Scatchard plot analysis giving a high constant of dissociation Kd 3.65 x 10(-10)/M and a low binding capacity of 0.88 x 10(-15) M/mg tissue. The test system is markedly dependent on temperature, incubation-time, and pH. The maximum binding was found at 37 degrees during 2 hr of incubation in a buffer of pH 7.5.

journal_name

Gen Comp Endocrinol

journal_title

General and comparative endocrinology

authors

Schlaghecke R

doi

10.1016/0016-6480(83)90143-0

subject

Has Abstract

pub_date

1983-02-01 00:00:00

pages

261-9

issue

2

eissn

0016-6480

issn

1095-6840

journal_volume

49

pub_type

杂志文章

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