当前位置： SCI文献检索 > GENERAL AND COMPARATIVE ENDOCRINOLOGY期刊下所有文献 > Identification of testosterone and progesterone in hemolymph of larvae of the fleshfly Sarcophaga bullata.

Identification of testosterone and progesterone in hemolymph of larvae of the fleshfly Sarcophaga bullata.

Abstract：

:Testosterone- and progesterone-like substances were detected by radioimmunoassay (RIA) in chromatographed extracts of hemolymph from larvae of Sarcophaga bullata (S.B.). Gas chromatographic (GC) analysis after heptafluorobutyric acid (HFBA) derivation of hemolymph extracts, purified by paper and silica gel column chromatography, showed a peak in the GC recordings with the same retention time as the HFBA derivative of pure testosterone. A testosterone concentration of 92 ng/100 ml hemolymph was found by GC; the concentration of progesterone, calculated on the basis of the RIA, was about two times higher. After preparing the o-pentafluorobenzyloxime (OPFB)-heptafluorobutyryl ester (HFB) derivatives of the hemolymph extracts, negative ion chemical ionization capillary gas chromatography-mass spectrometry (NCI/GC-MS) proved that hemolymph of larvae of the fleshfly Sarcophaga bullata indeed contains testosterone and progesterone. Several metabolites and precursors of the latter compounds could also be detected during the NCI/GC-MS analyses. Estrogens could not be traced by any of the methods we used. This is the first time that these steroids have been identified in insect hemolymph. These results add interesting perspectives for comparative endocrinology.

journal_name

Gen Comp Endocrinol

journal_title

General and comparative endocrinology

authors

De Clerck D,Eechaute W,Leusen I,Diederik H,De Loof A

doi

10.1016/0016-6480(83)90175-2

subject

Has Abstract

pub_date

1983-12-01 00:00:00

pages

368-78

issue

3

eissn

0016-6480

issn

1095-6840

journal_volume

52

pub_type

杂志文章

相关文献

GENERAL AND COMPARATIVE ENDOCRINOLOGY文献大全
  • Changes in plasma and gonadal steroid hormones in relation to the reproductive cycle and the sex inversion process in the protandrous seabass, Lates calcarifer.

    abstract：:Plasma and gonadal levels of several gonadal steroids (testosterone, 11-ketotestosterone, androstenedione, 11 beta-hydroxyandrostenedione, 17 beta-estradiol, and estrone) were measured by RIA in the protandrous seabass, Lates calcarifer, throughout an annual reproductive cycle. Twenty to 25 fish were killed every mont...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1006/gcen.1993.1170

    authors： Guiguen Y,Jalabert B,Thouard E,Fostier A

    更新日期：1993-12-01 00:00:00

  • Immunoreactive salmon calcitonin-like molecule in crustaceans: high concentrations in Nephrops norvegicus.

    abstract：:A salmon calcitonin (CT)-like peptide was characterized in various crustaceans by radioimmunological and radioreceptor assays. The highest levels of the molecule were found in the anterior part of the gut and the hepatopancreas of the Norway lobster: Nephrops norvegicus. Molecular sieving of this molecule suggested an...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/0016-6480(87)90163-8

    authors： Fouchereau-Peron M,Arlot-Bonnemains Y,Milhaud G,Moukhtar MS

    更新日期：1987-02-01 00:00:00

  • Involvement of neuropeptide Y Y1 receptors in the regulation of LH and GH cells in the pituitary of the catfish, Clarias batrachus: an immunocytochemical study.

    abstract：:Although neuropeptide Y (NPY) has been known to influence the release of luteinizing hormone (LH) and growth hormone (GH) from the pituitary gland of teleosts, the NPY receptor subtypes involved in the regulatory processes have not been fully defined. An attempt has been made to study the involvement of NPY Y1 recepto...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.ygcen.2006.05.012

    authors： Mazumdar M,Lal B,Sakharkar AJ,Deshmukh M,Singru PS,Subhedar N

    更新日期：2006-11-01 00:00:00

  • Molecular cloning, tissue distribution and expression profiles of thyroid hormone receptors during embryogenesis in orange-spotted grouper (Epinephelus coioides).

    abstract：:Three distinct thyroid hormone receptor (TR) cDNAs (gTRalphaA, gTRalphaB and gTRbeta) were cloned and sequenced in orange-spotted grouper (Epinephelus coioides). The cDNA of gTRalphaA consisted of 1555-bp and encoded a putative protein of 416 amino acids, while the cDNA of gTRalphaB consisted of 1459-bp and encoded 39...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.ygcen.2008.08.015

    authors： Tang X,Liu X,Zhang Y,Zhu P,Lin H

    更新日期：2008-11-01 00:00:00

  • Differential expression of IGF-I mRNA and peptide in the male and female gonad during early development of a bony fish, the tilapia Oreochromis niloticus.

    abstract：:Insulin-like growth factor I (IGF-I) plays a key role in the complex system that regulates bony fish growth, differentiation, and reproduction. The major source of circulating IGF-I is liver, but IGF-I-producing cells also occur in other organs, including the gonads. Because no data are available on the potential prod...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.ygcen.2005.11.008

    authors： Berishvili G,D'Cotta H,Baroiller JF,Segner H,Reinecke M

    更新日期：2006-05-01 00:00:00

  • Corticosterone levels during post-natal development in captive American kestrels (Falco sparverius).

    abstract：:We investigated post-natal development of the adrenocortical stress-response system in captive American kestrels (Falco sparverius) by measurements of baseline and stress-induced levels of corticosterone at ages 10, 16, 22, and 28 days post-hatching. Baseline levels of corticosterone increased significantly during pos...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0016-6480(02)00587-7

    authors： Love OP,Bird DM,Shutt LJ

    更新日期：2003-02-01 00:00:00

  • Spexin as a neuroendocrine signal with emerging functions.

    abstract：:Spexin (SPX), a novel peptide coevolved with the galanin/kisspeptin family, was first identified by bioinformatics prior to its protein purification/functional studies. Its mature peptide is highly conserved among different vertebrate classes. Based on the studies in mammals and fish models, SPX was found to be widely...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.ygcen.2018.01.015

    authors： Ma A,Bai J,He M,Wong AOL

    更新日期：2018-09-01 00:00:00

  • Serotonin interferes with Ca2+ and PKC signaling to reduce gonadotropin-releasing hormone-stimulated GH secretion in goldfish pituitary cells.

    abstract：:In goldfish, two endogenous gonadotropin-releasing hormones (GnRH), salmon GnRH (sGnRH) and chicken GnRH-II (cGnRH-II), are thought to stimulate growth hormone (GH) release via protein kinase C (PKC) and subsequent increases in intracellular Ca(2+) levels ([Ca(2+)](i)). In contrast, the signaling mechanism for seroton...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.ygcen.2008.07.021

    authors： Yu Y,Wong AO,Chang JP

    更新日期：2008-10-01 00:00:00

  • 11-Ketotestosterone suppresses interrenal activity in rainbow trout (Oncorhynchus mykiss).

    abstract：:Interrenal activity in relation to sexual maturity in male rainbow trout, and the effects of in vivo treatment with 11-ketotestosterone (11-KT) were investigated. Silastic pellets containing either 11-KT or no steroid were implanted in maturing male and immature male and female rainbow trout for a total of 11 weeks. T...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1006/gcen.1996.0125

    authors： Young G,Thorarensen H,Davie PS

    更新日期：1996-09-01 00:00:00

  • Neurohormonal control of growth and carbohydrate metabolism by the light green cells in Lymnaea stagnalis.

    abstract：:The neuroendocrine light green cells (LGCs), 4 clusters of together approximately 150 giant neurons in the cerebral ganglia of the freshwater gastropod, Lymnaea stagnalis, have been suggested to be involved in the control of growth. The present study examines in greater detail this role and possible actions on energy ...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/0016-6480(92)90068-u

    authors： Geraerts WP

    更新日期：1992-06-01 00:00:00

  • Development and control of the circadian pacemaker for melatonin release in the chicken pineal gland.

    abstract：UNLABELLED:Melatonin (MT) release from explanted pineal glands of 3- to 20-week-old chicken was investigated in a 5-day perifusion system. Both the chicken and the explanted glands were exposed to various environmental lighting regimens. OBSERVATIONS:(1) The explanted chicken pineal is sensitive to direct light. Conti...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1006/gcen.1997.7039

    authors： Csernus V,Ghosh M,Mess B

    更新日期：1998-04-01 00:00:00

  • Histochemical and immunocytochemical identification of the pituitary cell types in three sciaenid fishes: Atlantic croaker (Micropogonias undulatus), spotted seatrout (Cynoscion nebulosus), and red drum (Sciaenops ocellatus).

    abstract：:The adenohypophysial cell types in three species of sciaenid teleosts were identified using a combination of classical histochemical techniques and immunocytochemistry with antisera raised against piscine and human pituitary hormones. In general greater specificity for the different cell types was observed with antise...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/0016-6480(91)90086-l

    authors： Yan HY,Thomas P

    更新日期：1991-12-01 00:00:00

  • Plasma androgen levels, spermatogenesis, and secondary sexual characteristics in two species of plethodontid salamanders with dissociated reproductive patterns.

    abstract：:In the summer of 1991 (late May to early September), I measured seasonal levels of plasma androgens in two species of plethodontid salamanders to assess the temporal relationship of elevated plasma androgen levels with spermatogenic events and with sexual behavior. In both species, spermatogenesis occurs in the spring...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1006/gcen.1994.1175

    authors： Woodley SK

    更新日期：1994-11-01 00:00:00

  • Direct effects of neuropeptide nesfatin-1 on testicular spermatogenesis and steroidogenesis of the adult mice.

    abstract：:Recent studies have revealed nesfatin-1 as a hypothalamic neuropeptide, regulating food intake, energy expenditure and reproduction primarily by acting on the hypothalamic-pituitary-gonadal axis. Nesfatin-1 is also localized in several peripheral tissues including testes. However, functional significance of nesfatin-1...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.ygcen.2018.10.022

    authors： Ranjan A,Choubey M,Yada T,Krishna A

    更新日期：2019-01-15 00:00:00

  • Evidence for arylalkylamine N-acetyltransferase (AANAT2) expression in rainbow trout peripheral tissues with emphasis in the gastrointestinal tract.

    abstract：:In fish, melatonin is reported to be produced mainly in the pineal organ, but there is also evidence for the presence of melatonin in a number of extrapineal sites where it could act as an intracellular mediator or paracrine signal. The present study use the reverse transcription-polymerase chain reaction (RT-PCR) to ...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.ygcen.2006.12.008

    authors： Fernández-Durán B,Ruibal C,Polakof S,Ceinos RM,Soengas JL,Míguez JM

    更新日期：2007-06-01 00:00:00

  • Osmoregulatory effects of prolactin and growth hormone in embryonic chicks.

    abstract：:Intact White Leghorn chick embryos were treated daily (on Days 6-13) with bovine prolactin (PRL) or ovine growth hormone (GH) at doses of 4-10 micrograms/g embryo wet wt. A control group received an equal volume of avian saline. [Na+] and [Cl-] were determined in allantoic fluid samples taken on Days 10, 12, and 14, a...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/0016-6480(86)90059-6

    authors： Murphy MJ,Brown PS,Brown SC

    更新日期：1986-06-01 00:00:00

  • Relationships between CB1 cannabinoid receptors and pituitary endocrine cells in Xenopus laevis: an immunohistochemical study.

    abstract：:The distribution of the cannabinoid CB1 receptor and its relationships with individual endocrine cell types were investigated by immunohistochemistry in the anterior lobe of the Xenopus adenohypophysis. By use of a specific primary antibody raised in rabbit against the amino terminus of the rat CB1, we have found nume...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1006/gcen.2001.7720

    authors： Cesa R,Guastalla A,Cottone E,Mackie K,Beltramo M,Franzoni MF

    更新日期：2002-01-01 00:00:00

  • Isolation and biological characterization of a 6-kDa protein from hepatopancreas of lobster Panulirus argus with insulin-like effects.

    abstract：:A protein with insulin-like effects was isolated from the hepatopancreas of the lobster Panulirus argus following a classic method for mammalian insulin purification from the pancreas. After acid-alcoholic extraction and ethanol-ether precipitation followed by molecular filtration chromatography, a protein with an app...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0016-6480(03)00014-5

    authors： Gallardo N,Carrillo O,Moltó E,Deás M,González-Suárez R,Carrascosa JM,Ros M,Andrés A

    更新日期：2003-05-01 00:00:00

  • Stress-responsiveness influences baseline glucocorticoid levels: Revisiting the under 3min sampling rule.

    abstract：:Plasma glucocorticoid (CORT) levels collected within 3min of capture are commonly believed to reflect pre-stressor, baseline CORT levels. Differences in these "baseline" values are often interpreted as reflecting differences in health, or the amount of social and environmental stress recently experienced by an individ...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.ygcen.2017.01.028

    authors： Small TW,Bebus SE,Bridge ES,Elderbrock EK,Ferguson SM,Jones BC,Schoech SJ

    更新日期：2017-06-01 00:00:00

  • Zymosan-induced immune challenge modifies the stress response of hypoxic air-breathing fish (Anabas testudineus Bloch): Evidence for reversed patterns of cortisol and thyroid hormone interaction, differential ion transporter functions and non-specific imm

    abstract：:Fishes have evolved physiological mechanisms to exhibit stress response, where hormonal signals interact with an array of ion transporters and regulate homeostasis. As major ion transport regulators in fish, cortisol and thyroid hormones have been shown to interact and fine-tune the stress response. Likewise, in fishe...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.ygcen.2016.11.009

    authors： Simi S,Peter VS,Peter MCS

    更新日期：2017-09-15 00:00:00

  • The development and validation of a radioimmunoassay to measure plasma ACTH levels in salmonid fishes.

    abstract：:A radioimmunoassay (RIA) capable of determining blood ACTH levels in salmonid fishes was developed and validated. The RIA used an antibody raised against mammalian ACTH, iodinated human ACTH as tracer, and human 1-39 ACTH as standard. Incubation of the standard or unknown with antibody for 3 days before addition of as...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/0016-6480(86)90046-8

    authors： Sumpter JP,Donaldson EM

    更新日期：1986-06-01 00:00:00

  • Regional differences in the pituitary distribution of luteinizing hormone in the gonadectomized and proestrous female rat.

    abstract：:Previous data have shown that regional differences in the presence of anterior pituitary luteinizing hormone (LH) generally correlate with the comparable disparities in distribution of gonadotropes throughout the gland. In female rats, the differences are apparent over the estrous cycle, but are more prominent during ...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.ygcen.2008.02.015

    authors： Goldman JM,Stoker TE,Murr AS,McElroy WK,Cooper RL

    更新日期：2008-05-01 00:00:00

  • Effects of angiotensin II and C-type natriuretic peptide on the in situ perfused trunk preparation of the dogfish, Scyliorhinus canicula.

    abstract：:The renal roles of physiologically relevant doses of angiotensin II (Ang II) and C-type natriuretic peptide (CNP) were investigated in the dogfish, Scyliorhinus canicula, using an in situ perfused trunk preparation. Perfusion with 10(-9) M Ang II resulted in a glomerular antidiuresis and decreases in perfusate flow ra...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.ygcen.2005.08.002

    authors： Wells A,Anderson WG,Cains JE,Cooper MW,Hazon N

    更新日期：2006-01-15 00:00:00

  • Ecdysteroid levels in Daphnia magna during a molt cycle: determination by radioimmunoassay (RIA) and liquid chromatography-mass spectrometry (LC-MS).

    abstract：:Despite their abundance and their enormous significance for various ecological processes, endocrine systems of microcrustaceans have been poorly investigated. Here, we used a highly sensitive radioimmunoassay (RIA) to determine free and conjugated ecdysteroid levels in whole-body extracts of adult Daphnia magna during...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.ygcen.2006.11.015

    authors： Martin-Creuzburg D,Westerlund SA,Hoffmann KH

    更新日期：2007-03-01 00:00:00

  • Evolution of the sex-determining gene in the teleostean genus Oryzias.

    abstract：:In the genetic sex determination of vertebrates, the gonadal sex depends on the combination of sex chromosomes that a zygote possesses. Despite the discovery of the sex-determining gene (SRY/Sry) in mammals in 1990s, the sex-determining gene in non-mammalian vertebrates remained an enigma for over a decade. In most ma...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.ygcen.2015.10.004

    authors： Matsuda M,Sakaizumi M

    更新日期：2016-12-01 00:00:00

  • Thyroid hormone deiodinase type 2 mRNA levels in sea lamprey (Petromyzon marinus) are regulated during metamorphosis and in response to a thyroid challenge.

    abstract：:Thyroid hormones (THs) are crucial for normal vertebrate development and are the one obligate morphogen that drives amphibian metamorphosis. However, contrary to other metamorphosing vertebrates, lampreys exhibit a sharp drop in serum TH early in metamorphosis, and anti-thyroid agents such as potassium perchlorate (KC...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.ygcen.2012.12.007

    authors： Stilborn SS,Manzon LA,Schauenberg JD,Manzon RG

    更新日期：2013-03-01 00:00:00

  • Effects of dopamine on the adrenal gland of Podarcis sicula (Reptilia, Lacertidae).

    abstract：:The effects of dopamine administration on the adrenal gland of a lizard, Podarcis sicula, are described. Dopamine (0.7mg/100g body wt/day for 4 consecutive days) raised plasma ACTH and corticosterone levels (ACTH: from the basal level of 4.40+/-0.05-7.30+/-0.08pg/ml 24h after the fourth dopamine injection; corticoster...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.ygcen.2003.08.001

    authors： Capaldo A,Laforgia V,Sciarrillo R,De Falco M,Valiante S,Gay F,Virgilio F,Varano L

    更新日期：2004-01-01 00:00:00

  • Diurnal changes in plasma arginine vasotocin and isotocin in rainbow trout adapted to fresh water and brackish Baltic water.

    abstract：:The plasma levels of arginine vasotocin (AVT) and isotocin (IT) in rainbow trout (Oncorhynchus mykiss) were studied to assess possible diurnal variations in neurohypophysial nonapeptides. Fish were kept under natural photoperiod and adapted to fresh and brackish Baltic water. Blood was sampled at 5:00, 11:00, 16:00, 2...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1006/gcen.1996.0162

    authors： Kulczykowska E,Stolarski J

    更新日期：1996-11-01 00:00:00

  • Developmental changes in the properties of gonadotropin receptors in the ovarian follicles of amago salmon (Oncorhynchus rhodurus) during oogenesis.

    abstract：:The presence of specific, saturable, high-affinity gonadotropin receptors was demonstrated in membrane preparations from preovulatory ovarian follicles of amago salmon (Oncorhynchus rhodurus), including intact follicles, isolated thecal layers, and isolated granulosa cells. Optimum conditions for the binding study usi...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/0016-6480(88)90177-3

    authors： Kanamori A,Nagahama Y

    更新日期：1988-10-01 00:00:00

  • Acute exposure to physiological doses of triiodothyronine does not induce gonadal caspase 3 activity in goldfish in vitro.

    abstract：:Seasonally spawning fish rely on a dynamic and complex hormonal interplay to regulate cycles of gonadal development and the regression. Thyroid hormones have been shown to be a key player during gonadal development, and can regulate the activity of a number of essential reproductive hormones. Apoptosis is a vital cell...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.ygcen.2019.113382

    authors： Allan ERO,Dores CB,Nelson ER,Habibi HR

    更新日期：2020-04-01 00:00:00