Abstract:
:Dopamine receptors in the pituitary of the African catfish, Clarias gariepinus, were characterized using [3H]spiperone as radioligand. Specific binding of [3H]spiperone to pituitary membranes reached equilibrium within 60 min of incubation. The binding of the radioligand was tissue specific since the amount of binding was linear with pituitary membrane content in the incubations. In addition, pituitary membranes were observed to bind considerably more [3H]spiperone, compared to membrane preparation of various other tissues. Saturation experiments revealed the presence of a single class of high affinity/low capacity binding sites. The binding characteristics, estimated by Scatchard analysis, were: Kd = 3.2 +/- 0.5 x 10(-9) M and Bmax = 105 +/- 5 fmol/mg protein. Specific binding was displaceable with dopamine and with various specific D2 agonists and antagonists. The nature of displacement curves resembles those observed in studies on mammalian dopamine receptors. Binding experiments with cell fractions, obtained after centrifugation of dispersed pituitary cells over a Percoll density gradient, showed that most [3H]spiperone binding was obtained in an enriched gonadotropic cell fraction. This observation indicates that the receptor characteristics, estimated with the [3H]spiperone assay, are representative for dopamine receptors on the gonadotropic cells.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Van Asselt LA,Goos HJ,De Leeuw R,Peter RE,Hol EM,Wassenberg FP,Van Oordt PGdoi
10.1016/0016-6480(90)90154-esubject
Has Abstractpub_date
1990-10-01 00:00:00pages
107-15issue
1eissn
0016-6480issn
1095-6840pii
0016-6480(90)90154-Ejournal_volume
80pub_type
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