当前位置： SCI文献检索 > GENERAL AND COMPARATIVE ENDOCRINOLOGY期刊下所有文献 > Molecular cloning of the cDNAs encoding two gonadotropin beta subunits (GTH-I beta and -II beta) from the goldfish, Carassius auratus.

Molecular cloning of the cDNAs encoding two gonadotropin beta subunits (GTH-I beta and -II beta) from the goldfish, Carassius auratus.

Abstract：

:Two types of cDNAs (GTH-I beta and -II beta) that encode the beta subunit of goldfish gonadotropin were cloned from a goldfish pituitary cDNA library. The nucleotide sequence of GTH-I beta cDNA was 616 bp long, encoding 130 amino acids, and that of GTH-II beta was 552 bp long, encoding 140 amino acids. These two types of goldfish GTH beta subunits showed an identity of 52% in the nucleotide sequence and 38% in the amino acid sequence. When compared to mammalian GTH beta subunits, goldfish GTH-I beta showed higher homology to FSH-beta s than LH-beta s, and goldfish-II beta to LH-beta s. Genomic Southern blot analysis revealed that cyprinid species harbored genes which were homologous to goldfish GTH-I beta and -II beta, demonstrating the duality of GTH in cyprinid fish as has been shown in other teleost fish. Northern blot analysis of GTH-I beta and -II beta mRNAs in goldfish at differing stages of ovarian maturity (immature, maturing, mature, and regressed) showed that both GTH-I beta and -II beta mRNA levels increased with the progression of maturity and declined in regressed stage. These results suggested that gonadal development in the goldfish was regulated by the two GTHs.

journal_name

Gen Comp Endocrinol

journal_title

General and comparative endocrinology

authors

Yoshiura Y,Kobayashi M,Kato Y,Aida K

doi

10.1006/gcen.1996.6839

subject

Has Abstract

pub_date

1997-03-01 00:00:00

pages

379-89

issue

3

eissn

0016-6480

issn

1095-6840

pii

S0016-6480(96)96839-2

journal_volume

105

pub_type

杂志文章

相关文献

GENERAL AND COMPARATIVE ENDOCRINOLOGY文献大全