Effect of acute and chronic insulin administrations on major factors involved in the control of muscle protein turnover in rainbow trout (Oncorhynchus mykiss).

Abstract:

:In this study, the effect of acute and chronic insulin treatments on major factors involved in the control of muscle protein turnover were investigated in rainbow trout (Oncorhynchus mykiss). We found that acute but not chronic insulin administration leads to the induction of the phosphorylation of several key factors (IRS1, TOR and 4E-BP1) involved in the control of the protein synthesis and to the concomitant down-regulation of the expression of ubiquitin-proteasome-related genes (atrogin1, C2, C9) and the calpains inhibitor calpastatin. In contrast, no modification of autophagy-related gene (LC3B, gabarpl1, atg4b) expressions was observed suggesting that the mechanisms controlling this proteolytic route have diverged throughout the evolution. Overall, these results provide a possible explanation of the growth-promoting properties of insulin previously described in fish and indicate that this hormone acutely administrated is able to exert a regulatory influence on various factors associated with growth in skeletal muscle.

journal_name

Gen Comp Endocrinol

authors

Seiliez I,Panserat S,Skiba-Cassy S,Polakof S

doi

10.1016/j.ygcen.2011.03.026

subject

Has Abstract

pub_date

2011-07-01 00:00:00

pages

363-70

issue

3

eissn

0016-6480

issn

1095-6840

pii

S0016-6480(11)00138-9

journal_volume

172

pub_type

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