Abstract:
:125I-labeled bovine and tilapia growth hormones were used to assess the presence of growth hormone receptors in membranes prepared from tissues of the tilapia Oreochromis mossambicus. The highest level of specific binding was detected in liver membranes from animals of both sexes and the binding was protein-dependent. Tilapia growth hormone, bovine growth hormone, and ovine prolactin, but not tilapia prolactin, potently inhibited the hepatic binding of 125I-labeled bovine growth hormone. Scatchard analysis of the 125I-labeled bovine growth hormone binding data revealed a Bmax (maximum binding) value of 180 fmol/mg protein and a Kd (dissociation constant) value of 13 nM. Tilapia growth hormone potently inhibited hepatic binding of 125I-labeled tilapia growth hormone. Scatchard analysis revealed a single class of binding sites with Bmax and Kd values of 390 fmol/mg protein and 2.5 nM, respectively. Bovine growth hormone and ovine prolactin were less potent while tilapia prolactin was inactive in inhibiting hepatic 125I-labeled tilapia growth hormone binding.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Ng TB,Leung TC,Cheng CH,Woo NYdoi
10.1016/0016-6480(92)90132-4keywords:
subject
Has Abstractpub_date
1992-04-01 00:00:00pages
111-8issue
1eissn
0016-6480issn
1095-6840pii
0016-6480(92)90132-4journal_volume
86pub_type
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