Abstract：
:A triacetyl form of α-melanocyte-stimulating hormone (MSH) was found in carp (Cyprinus carpio) and goldfish (Carassius auratus), by selective detection of mass profile for cell secretory granules using direct tissue matrix-assisted laser desorption ionization with time-of-flight mass spectrometry (MALDI-TOF MS) analysis during the investigation of fish pituitaries. The structure of triacetyl-α-MSH in carp and goldfish was further analyzed using a collision-induced dissociation with electrospray ionization mass spectrometry, and determined to be N,O-diacetyl Ser as the N-terminal residue and O-acetyl Tyr at position 2. These modifications for α-MSH in carp and goldfish are structurally different from that of medaka hormone, in which [N,O-diacetyl Ser(1), O-acetyl Ser(3)]-α-MSH has been identified. The profiles of four α-MSH variants, des-, mono-, di- and tri-acetyl forms in goldfish and medaka pituitaries were also examined by direct tissue MALDI-TOF MS analysis, and the percentages as a total of α-MSH molecules were compared for fish reared in a white or black tank for 5 days. Among structural variants, diacetyl-α-MSH was the predominant form in goldfish and N-desacetyl-α-MSH in medaka, respectively. In both species, the relative level of the predominant form in the pituitary of white-adapted fish tended to be lower than that of black-adapted fish. In goldfish, no significant difference was observed in the relative content of triacetyl-α-MSH in both backgrounds, whereas the lowest content of triacetyl-α-MSH was found in black-adapted medaka. These preliminary data indicate that it is difficult to elucidate the relations between the physiological roles and acetylated pattern of α-MSH molecule, depending on species.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Yasuda A,Tatsu Y,Shigeri Ydoi
10.1016/j.ygcen.2011.11.009subject
Has Abstractpub_date
2012-01-15 00:00:00pages
270-6issue
2eissn
0016-6480issn
1095-6840pii
S0016-6480(11)00423-0journal_volume
175pub_type
杂志文章abstract：:In teleosts, lungfish, amphibians, and a reptile, Amphibolurus nuchalis, hormonal stimulation of hepatic glycogenolysis is mediated by a rise in intracellular cyclic AMP concentration. In mammals, by contrast, the inositol trisphosphate/Ca2+/diacylglycerol signal transduction pathways are also involved. The present st...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(92)90200-4
更新日期：1992-10-01 00:00:00
abstract：:In goldfish, two endogenous gonadotropin-releasing hormones (GnRH), salmon GnRH (sGnRH) and chicken GnRH-II (cGnRH-II), are thought to stimulate growth hormone (GH) release via protein kinase C (PKC) and subsequent increases in intracellular Ca(2+) levels ([Ca(2+)](i)). In contrast, the signaling mechanism for seroton...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2008.07.021
更新日期：2008-10-01 00:00:00
abstract：:Skin pigmentation pattern is a species-specific characteristic that depends on the number and the spatial combination of several types of chromatophores. This feature can change during life, for example in the metamorphosis or reproductive cycle, or as a response to biotic and/or abiotic environmental cues (nutrition,...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2020.113662
更新日期：2021-01-15 00:00:00
abstract：:The importance of late winter pairing activity on the neuroendocrinology of wild waterfowl is unknown. In this study, we examined the sex-related differences in the roles of late winter pairing activity and seasonal influences on neuroendocrine and reproductive physiology in both male and female mallards. Our main goa...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.2000.7469
更新日期：2000-05-01 00:00:00
abstract：:Arginine vasotocin (AVT) and oxytocin (OXY), in the dose range of 2 X 10(-13)-2 X 10(-8) mol, were found to be potent vasoconstrictors of the arterioarterial pathway of isolated perfused eel gills. AVT was significantly (P less than 0.05) more potent than OXY, as indicated by the results of both dose- and concentratio...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(86)90028-6
更新日期：1986-10-01 00:00:00
abstract：:The presence of a robust estrogen (E2) response system throughout heart and blood vessel tissues of vertebrates, including humans, has led to the speculation that this ubiquitous hormone may play a prominent role in the health and maintenance of the adult cardiovascular system (CVS). We previously established an embry...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2013.04.016
更新日期：2013-08-01 00:00:00
abstract：:Rapid progestin effects on sperm physiology have been described in a variety of vertebrate species. Here, we briefly review the signaling pathways mediating rapid progestin induction of sperm hypermotility and increased fertility in two teleost species, Atlantic croaker and southern flounder. Acute in vitro treatment ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.ygcen.2018.12.002
更新日期：2019-08-01 00:00:00
abstract：:Selected physiological parameters were monitored over a 4-year period in the Barrow Island euro, Macropus robustus isabellinus, in Western Australia in a study of this species' homeostatic capabilities in an extremely arid habitat where individuals are exposed to high environmental temperatures and a lack of free wate...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2010.02.018
更新日期：2010-05-15 00:00:00
abstract：:In mammals, leptin plays an important role in puberty and reproduction and leptin is regulated by sex steroids. Elevated leptin levels have been associated with sexual maturation in some teleosts such as Atlantic salmon. In the present study, primary cultures of Atlantic salmon hepatocytes were used to investigate the...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2015.01.015
更新日期：2015-09-15 00:00:00
abstract：:The role of ovarian hormones in the expression of parental behavior and in the regulation of LH secretion was investigated in incubating commercial meat-type hens. After ovariectomy, incubating hens continued to incubate eggs normally and brooded day-old chicks given to replace eggs, in a manner similar to sham-ovarie...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1996.0013
更新日期：1996-01-01 00:00:00
abstract：:In the last two decades, maternally derived yolk androgens have been shown to significantly alter offspring development, and a number of these effects persist into adulthood. However, little is known about their underlying mechanisms. Mechanisms that have been suggested are changes in the endogenous androgen productio...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2013.10.014
更新日期：2014-01-01 00:00:00
abstract：:We investigated the effect of several potential carbohydrate secretagogues, amino acids, a ketoacid, and potassium chloride on insulin, glucagon, and somatostatin release from the in vitro perfused Brockmann body of channel catfish (Ictalurus punctatus). Mannose (15 mM) stimulated the release of insulin and somatostat...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(87)90120-1
更新日期：1987-03-01 00:00:00
abstract：:Salmonids have four subtypes of insulin-like growth factor binding protein (IGFBP)-1, termed -1a1, -1a2, -1b1 and 1b2, owing to teleost- and a lineage-specific whole-genome duplications. We have previously produced recombinant proteins of masu salmon IGFBP-1a1 and -1b2 and conducted functional analysis. To further cha...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2020.113606
更新日期：2020-12-01 00:00:00
abstract：:The discovery of novel members of the CRF neuropeptide family, urocortin 1 (Ucn1), urocortin 2 and 3 has provided important insights into stress adaptation pathways, and predicted that stress adaptation involves more systems than the HPA-axis alone. This mini-review aims to summarize our recent data and research by ot...
journal_title：General and comparative endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.ygcen.2007.04.005
更新日期：2007-08-01 00:00:00
abstract：:The effect of salmon calcitonin (0.25 MRC mU/g body wt) was investigated on the serum calcium and inorganic phosphate levels of the frog. Rana tigrina. The hormone evokes hypocalcemia (on Day 1 and Day 3) which is followed by a significant hypercalcemia on Day 10. Thereafter, the level of calcium decreases again on Da...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(89)90109-3
更新日期：1989-04-01 00:00:00
abstract：:The binding of 125I-labeled ovine prolactin (oPRL) to membrane preparations of several tissues from the male green frog, Rana esculenta, collected during the year is reported. PRL binding to kidney fractions was generally high (range 5-45%). A maximum was observed in the month of October, whereas the lowest value was ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(87)90053-0
更新日期：1987-10-01 00:00:00
abstract：:Atrazine (ATZ) is a widely used herbicide and is considered an endocrine disruptor of different organisms. However, the molecular interactions of ATZ with biological targets in mammalian endocrine systems are not understood fully. In the present study, we observed that ATZ administration (50, 100 and 200 mg/kg) for 3 ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2013.01.010
更新日期：2013-04-01 00:00:00
abstract：:Pancreatic polypeptide (PP) has been isolated from pancreatic extracts of the herring gull (Larus argentatus) using a radioimmunoassay employing an antiserum (PP 221), generated to the conserved C-terminal hexapeptide amide of mammalian PP. Gel permeation and reverse-phase HPLC fractionation of crude pancreatic extrac...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1994.1029
更新日期：1994-02-01 00:00:00
abstract：:The avian neurohypophyseal hormone arginine vasotocin (AVT) is known to be involved in the regulation of adrenocorticotropin (ACTH) release by interacting with the VT2 receptor (VT2R), which is homologous to the mammalian vasopressin V1b receptor (V1bR). To study the role of glucocorticoid in the expression and regula...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2008.12.019
更新日期：2009-04-01 00:00:00
abstract：:The effects of salmon gonadotropin-releasing hormone (sGnRH) and the superactive agonist [D-Arg6, Pro9NEt]-sGnRH (sGnRH-A) on growth hormone (GH) and gonadotropin (GtH) release were examined using a perifusion system for pituitary fragments of the common carp (Cyprinus carpio). Perifusion of 2-min pulses of different ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1993.1009
更新日期：1993-01-01 00:00:00
abstract：:The skin of Xenopus laevis, because of its high concentrations of amidated peptides, is an excellent model for the study of neuropeptide biosynthesis. In this paper we determined levels of the mRNAs which encode cerulin and characterized the enzymatic activity which likely produces the carboxyl terminal alpha-amide mo...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(87)90206-1
更新日期：1987-07-01 00:00:00
abstract：:Chronic confinement for 1 month caused a significant elevation of plasma cortisol but suppressed the levels of plasma testosterone and 11-ketotestosterone in sexually mature male brown trout. An acute handling stress for 1 hr elevated blood cortisol and ACTH levels and also suppressed circulating androgens. This andro...
journal_title：General and comparative endocrinology
pub_type： 杂志文章,评审
doi：10.1016/0016-6480(87)90036-0
更新日期：1987-11-01 00:00:00
abstract：:Growth hormone (GH) regulates essential physiological functions in teleost fishes, including growth, metabolism, and osmoregulation. Recent studies have identified two clades of putative receptors for GH (GHR1 clade and GHR2 clade) in fishes, both of which are highly expressed in the liver. Moreover, the liver is an i...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2012.07.010
更新日期：2012-10-01 00:00:00
abstract：:The effect of thyrotropin releasing hormone (TRH) administered intramuscularly (im) on serum levels of prolactin (Prl) in ovariectomized (ovx) adult turkeys before and following the onset of photostimulation, before and during daily administration (im) of progesterone (P; 0.1, 0.4, or 1.0 mg/kg), estradiol benzoate (E...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(86)90101-2
更新日期：1986-04-01 00:00:00
abstract：:An enzyme-linked immunosorbent assay (ELISA) has been developed for a conjugated bile acid, 7alpha,12alpha,24-trihydroxy-5alpha-cholan-3-one 24-sulfate (commonly referred to as 3-keto petromyzonol sulfate [3kPZS]), a pheromone released by reproductively mature male sea lampreys to attract sexually mature females. A po...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/s0016-6480(02)00517-8
更新日期：2002-12-01 00:00:00
abstract：:Serotonin (5-HT) is well known for modulating the release of GnRH and gonadotropin in teleosts. Reports on increased female:male ratio after the blockade of 5-HT biosynthesis proposed a role for 5-HT in brain sex differentiation. Two types of tryptophan hydroxylase (Tph), rate-limiting enzyme in the biosynthesis of 5-...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2009.11.009
更新日期：2010-04-01 00:00:00
abstract：:Differential transfer of maternal testosterone (T) into egg yolk provides a means of adjusting an offspring's phenotype to ambient environmental conditions. While the environmental and genetic driven variability in yolk T levels is widely described, the underlying mechanisms are poorly understood. Here, we investigate...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2017.05.004
更新日期：2018-01-15 00:00:00
abstract：:The Harderian gland of the lizard Podarcis sicula sicula (Raf) contains connective tissue type mast cells whose numbers vary during the year showing two peaks, one in spring the other in winter. No sex differences are found throughout the year. Thermal and photoperiodic manipulations indicate that only temperature inf...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1997.6925
更新日期：1997-09-01 00:00:00
abstract：:The conversion in vitro of cholesterol (Ch) to nonpolar metabolites and ecdysteroids was studied in Y-organs of the xanthid crab, Menippe mercenaria. In one set of experiments, Y-organs were prelabeled in vivo by injecting crabs with 100 microCi of [3H]Ch, and halved glands were then incubated for 24 and 48 hr in the ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(92)90255-i
更新日期：1992-11-01 00:00:00
abstract：:Gonadotropin-releasing hormone (GnRH) was detected in the brains of passerine birds, a recently evolved and diverse avian group. The molecular forms of GnRH in two species of birds under breeding conditions were deduced using methods of HPLC and immunology. The brain extracts of song sparrows (Melospiza melodia) conta...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(88)90024-x
更新日期：1988-03-01 00:00:00
