Abstract：
:Effects of epidermal growth factor (EGF) and possible mechanisms of EGF-mediated signal transduction were investigated in isolated cells of the digestive gland of the mussel (Mytilus galloprovincialis Lam. ). EGF induced a cytosolic Ca2+ transient and subsequently stimulated DNA synthesis; both effects were dose-dependent in the nanomolar range and inhibited by pretreatment with an inhibitor of tyrosine kinase activity, suggesting specific EGFR-like receptors. The EGF-induced cytosolic Ca2+ transient was mainly due to a Ca2+ influx through the plasma membrane, possibly involving voltage-insensitive Ca2+ channels. Such a Ca2+ response was abolished by pretreatment with indomethacin and NDGA, inhibitors of arachidonic acid metabolism; similarly, the EGF-stimulated increase in DNA synthesis was significantly reduced. Indomethacin, a cyclooxygenase inhibitor, had the greatest effect on both EGF-induced responses. Results suggest the presence of EGF-responsive cells in the mussel digestive gland. A possible role for arachidonic acid and its metabolites in mediating the effects of EGF is also indicated.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Canesi L,Ciacci C,Orunesu M,Gallo Gdoi
10.1006/gcen.1997.6918subject
Has Abstractpub_date
1997-08-01 00:00:00pages
221-8issue
2eissn
0016-6480issn
1095-6840pii
S0016648097969185journal_volume
107pub_type
杂志文章abstract：:Thyroid stimulating hormone (TSH) is produced by the anterior pituitary and is used to regulate thyroid hormone output, which in turn controls metabolic activity. Currently, the pituitary is believed to be the only source of TSH used by the thyroid. Recent studies in mice from our laboratory have identified a TSHbeta ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2009.04.006
更新日期：2009-07-01 00:00:00
abstract：:Stress is a known inhibitor of reproductive function. The mechanisms by which stress acts to influence the reproductive axis have been intensely studied and appear to be extremely varied. Gonadotropin-releasing hormone (GnRH) is a critical component of the vertebrate reproductive axis and directly causes pituitary gon...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2008.05.010
更新日期：2008-07-01 00:00:00
abstract：:Catechol-O-methyltransferase (COMT) is involved in the methylation and inactivation of endogenous and xenobiotic catechol compounds, and serves as a common biochemical link in the catecholamine and catecholestrogen metabolism. Studies on cloning, sequencing and function characterization comt gene in lower vertebrates ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2016.12.001
更新日期：2017-05-15 00:00:00
abstract：:The differential impact of stress on brain functions of males and females has been widely observed in vertebrates. Recent evidence suggests that stress-induced glucocorticoid signaling affects sexual differentiation and sex changes in teleost fish. These facts led us to postulate that there were sex differences in glu...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2015.09.031
更新日期：2015-11-01 00:00:00
abstract：:Four genomes and two transcriptomes from six Chelicerate species were analyzed for the presence of neuropeptide and neurohormone precursors and their GPCRs. The genome from the spider Stegodyphus mimosarum yielded 87 neuropeptide precursors and 101 neuropeptide GPCRs. High neuropeptide transcripts were also found in t...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2015.07.014
更新日期：2016-11-09 00:00:00
abstract：:In anaesthetized young (6 weeks old) and adult (22-30 weeks old) domestic fowl, the administration of thyrotrophin releasing hormone (TRH; 1.0 micrograms/kg in young birds; 10.0 micrograms/kg in adults) or human pancreatic growth hormone-releasing factor (hpGRF(1-44)NH2; 10.0 micrograms/kg in both cases) markedly incr...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(85)90413-7
更新日期：1985-07-01 00:00:00
abstract：:Gonadotropin-inhibitory hormone (GnIH) is an inhibitor of the hypothalamic-pituitary-gonadal (HPG) axis. GnIH is also called RFamide-related peptide (RFRP) as GnIH peptides have a characteristic C-terminal LPXRFiamide (X = L or Q) sequence. GnIH is thought to be the mediator of stress by negatively regulating the HPG ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.ygcen.2018.03.004
更新日期：2018-09-01 00:00:00
abstract：:Estrogen signalling is critical for ovarian differentiation in reptiles with temperature-dependent sex determination (TSD). To elucidate the involvement of estrogen in this process, adrenal-kidney-gonadal (AKG) expression of estrogen receptor (ERα) was studied at female-producing temperature (FPT) in the developing em...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2014.08.003
更新日期：2015-09-01 00:00:00
abstract：:Unlike mammals, the goldfish is unique in having dopamine (DA) D1 receptors in the anterior pituitary. In this species, DA stimulates growth hormone (GH) release via D1 receptors coupled to the cAMP-dependent pathway. To further examine the postreceptor mechanisms of this novel pituitary DA D1 system, the role of extr...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1994.1088
更新日期：1994-06-01 00:00:00
abstract：:In songbirds, neurons that regulate learned song behavior undergo extensive seasonal plasticity in their number and size in relation to the bird's reproductive status. Seasonal plasticity of these brain regions is primarily regulated by changes in circulating concentrations of testosterone. Androgen receptors are pres...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2009.08.001
更新日期：2010-03-01 00:00:00
abstract：:Y-Organs and control tissues from intact (intermolt) and 48-hr de-eyestalked Cancer antennarius donors were cultured for 12 and 24 hr in crustacean saline supplemented 10% with crab serum and containing [14C]cholesterol. Under these conditions, Y-organs took up significantly more [14C]cholesterol than ovary or muscle,...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(85)90215-1
更新日期：1985-03-01 00:00:00
abstract：:The effect of salmon calcitonin (0.25 MRC mU/g body wt) was investigated on the serum calcium and inorganic phosphate levels of the frog. Rana tigrina. The hormone evokes hypocalcemia (on Day 1 and Day 3) which is followed by a significant hypercalcemia on Day 10. Thereafter, the level of calcium decreases again on Da...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(89)90109-3
更新日期：1989-04-01 00:00:00
abstract：:Plasma and gonadal levels of several gonadal steroids (testosterone, 11-ketotestosterone, androstenedione, 11 beta-hydroxyandrostenedione, 17 beta-estradiol, and estrone) were measured by RIA in the protandrous seabass, Lates calcarifer, throughout an annual reproductive cycle. Twenty to 25 fish were killed every mont...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1993.1170
更新日期：1993-12-01 00:00:00
abstract：:The role of the pituitary in regulating interrenal activity in coho salmon (Oncorhynchus kisutch) was examined using short- (1 week) or long- (10 week) term hypophysectomized (Hx) animals. In experiments in which Hx animals were injected with ovine growth hormone (oGH), triiodothyronine, or vehicle, plasma cortisol wa...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1993.1179
更新日期：1993-12-01 00:00:00
abstract：:A highly specific and homologous radioimmunoassay (RIA) for the measurement of prolactin (PRL) in the plasma and the pituitary of the eel was developed using a rabbit antiserum to eel PRL. PRL was purified from the pituitary of Japanese eel (Anguilla japonica). Pituitary extracts and plasma from the Japanese and Europ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(91)90167-5
更新日期：1991-03-01 00:00:00
abstract：:Daily variations in circulating melatonin concentrations have been measured at monthly intervals from April to December 1986 in an Antarctic bird, the emperor penguin, Aptenodytes forsteri, maintained under natural conditions. Both duration of the elevated nighttime melatonin levels and amplitude of the day-night rhyt...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(91)90048-b
更新日期：1991-11-01 00:00:00
abstract：:This review focuses on the endocrine regulation of reproductive behavior in moth species with particular emphasis on Helicoverpa spp. Reproductive behavior in most adult moths is dependent on the release of a unique blend of sex pheromones by the females to attract conspecific males. Mating, on the other hand, results...
journal_title：General and comparative endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.ygcen.2008.04.004
更新日期：2009-05-15 00:00:00
abstract：:Application of alpha-MSH immunohistochemistry to the brain of elasmobranchs (Scyliorhinus torazame and Etmopterus brachyurus) demonstrated a marked species difference concerning the distribution of the alpha-MSH-like molecule in the brain. In S. torazame, alpha-MSH-like immunoreactive cells were present in the hypotha...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.2001.7628
更新日期：2001-06-01 00:00:00
abstract：:The skin of Xenopus laevis, because of its high concentrations of amidated peptides, is an excellent model for the study of neuropeptide biosynthesis. In this paper we determined levels of the mRNAs which encode cerulin and characterized the enzymatic activity which likely produces the carboxyl terminal alpha-amide mo...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(87)90206-1
更新日期：1987-07-01 00:00:00
abstract：:Intraperitoneal injection of rainbow trout (Salmo gairdneri) with 0.4 microgram/g of human growth hormone (hGH) increased plasma levels of 3,5,3'-triiodo-L-thyronine (T3) and Vmax of the hepatic microsomal 5'-monodeiodinase enzyme (5'D) that converts thyroxine (T4) to T3, with no effect on Km or plasma T4 levels. A do...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(90)90002-4
更新日期：1990-05-01 00:00:00
abstract：:This study examines the regulation of thyroid activity in adult rough-skinned newts, Taricha granulosa. Aquatic, breeding-condition newts were hypophysectomized and were injected for 23 days with the mammalian adenohypophysial hormones: ACTH, PRL, and GH. During a fourth week of treatment, these newts also received TS...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(85)90385-5
更新日期：1985-08-01 00:00:00
abstract：:Undifferentiated spermatogonia (Aund) or spermatogonial stem cells (SSCs) are committed to the establishment and maintenance of spermatogenesis and fertility throughout a male's life and are located in a highly specialized microenvironment called niche that regulates their fate. Although several studies have been deve...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2018.06.019
更新日期：2019-03-01 00:00:00
abstract：:Primary cultured gill epithelia from goldfish and rainbow trout were used to investigate a role for cortisol in the regulation of paracellular permeability and tight junction (TJ) protein transcript abundance in representative stenohaline versus euryhaline freshwater (FW) fish gills. Glucocorticoid and mineralocortico...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2011.04.023
更新日期：2011-07-01 00:00:00
abstract：:Histochemical assays, hormonal quantitation, and steroid biosynthetic studies were carried out with adrenal glands obtained from four stranded whales of two different species (Kogia breviceps and Mesoplodon europaeus), and selected comparisons were made with the results of similar studies of adrenals from terrestrial ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(87)90041-4
更新日期：1987-11-01 00:00:00
abstract：:To better understand the role(s) of progesterone in fish spermatogenesis, we cloned the nuclear progesterone receptor (Pgr) of Atlantic cod. The open-reading frame of the cod pgr consists of 2076 bp, coding for a 691-amino acids-long protein that shows the highest similarity with other piscine Pgr proteins. Functional...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2012.07.022
更新日期：2012-10-01 00:00:00
abstract：:Optimization of food consumption and ultimately growth are major concerns for aquaculture. In fish, food intake is regulated by several hormones produced by both brain and peripheral tissues. Changes in feeding behavior and appetite usually occur through the modulation of the gene expression and/or action of these app...
journal_title：General and comparative endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.ygcen.2009.09.001
更新日期：2010-07-01 00:00:00
abstract：:Plasma glucagon levels in 11 different teleosts and in dogfish were evaluated using an adapted classical mammalian radioimmunoassay (RIA). The values obtained are considered relative; sensitivity was inferior to the original method, but the validation experiment results are acceptable. The mean value of the coefficien...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(86)90131-0
更新日期：1986-09-01 00:00:00
abstract：:In fish there are few data on the gonadotropin-releasing hormone (Gn-RH) neurosecretory activity, which could explain long- and short-term variations of the gonadotropin secretion. There is no biological species specificity between mammal and fish Gn-RH; although there is a structural difference, they are, on the cont...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(86)90255-8
更新日期：1986-01-01 00:00:00
abstract：:Due to its unique organization, the teleost pituitary is an ideal model in which to investigate the relationship of the nervous system with the pituitary endocrine cells. A light microscope immunocytochemical study of the sea bass pituitary revealed six different neuropeptides in nerve fibers which projected into the ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(88)90200-6
更新日期：1988-11-01 00:00:00
abstract：:The Lottia gigantea genome was prospected for the presence of genes coding neuropeptides and neurohormones. Four genes code insulin-related peptides: two genes code molluscan insulin-like growth hormones, one gene an insulin very similar to vertebrate insulin, and the fourth a peptide related to drosophila insulin-lik...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2010.02.010
更新日期：2010-05-15 00:00:00
