Frog ANP increases the amiloride-sensitive Na(+) channel activity in urinary bladder cells of Japanese tree frog, Hyla japonica.

Abstract:

:We examined the mechanism of amiloride-sensitive Na(+) channels (ENaC) activated by fANP in epithelial cells of frog urinary bladder by using a cell-attached patch-clamp technique. ENaC activities in the epithelial cells were significantly increased following administration of both 10(-9)M fANP and 10(-5)M 8-Br-cGMP. Both fANP and 8-Br-cGMP, however, failed to activate the ENaC in the presence of 10(-6)M amiloride. In addition, 8-Br-cGMP failed to activate the ENaC in the presence of a PKA inhibitor, KT-5720. In the next experiment, we measured both cGMP and cAMP production levels after treatment of fANP on the frog urinary bladder cells. Frog ANP significantly increased cGMP production, but not the cAMP production. Taken together, these results suggest that fANP activates ENaC through increases in cGMP production and activation of PKA.

journal_name

Gen Comp Endocrinol

authors

Yamada T,Matsuda K,Uchiyama M

doi

10.1016/j.ygcen.2006.11.003

subject

Has Abstract

pub_date

2007-06-01 00:00:00

pages

286-8

issue

2-3

eissn

0016-6480

issn

1095-6840

pii

S0016-6480(06)00338-8

journal_volume

152

pub_type

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