Different actions of ecdysis-triggering hormone on the brain and ventral nerve cord of the hornworm, Manduca sexta.

Abstract:

:Ecdysis, or the shedding of the old cuticle, depends on coordinated stereotyped behaviors, regulated by a number of neuropeptides. In the hornworm, Manduca sexta, two neuropeptides interact, namely ecdysis-triggering hormone (ETH) and eclosion hormone. We looked at the effects of ETH in vivo and in vitro, on the brain and the ventral nerve cord to determine the roles played by these hormones. We monitored ecdysis onset and the presence of cGMP and eclosion hormone immunoreactivity. In vivo, only a fraction of larvae lacking the cell bodies containing eclosion hormone, and injected with ETH, were able to undergo ecdysis, with a delayed response. These animals showed strongest cGMP immunoreactivity in the subesophageal and thoracic ganglia, with concomitant reductions in eclosion hormone immunoreactivity in descending axons in comparison with animals not undergoing ecdysis. Animals lacking the brain showed reduced to no cGMP levels in all ganglia. In vitro, isolated CNS preparations lacking the brain initiated ecdysis motor programs after incubation in ETH, with faster onset times than controls, and with reduced cGMP immunoreactivity. If ETH was applied only to the brain of the isolated CNS, cGMP immunoreactivity was noted primarily in the subesophageal and thoracic ganglia, with a decrease in eclosion hormone immunoreactivity in descending axons. ETH addition to the rest of the nerve cord showed reduced eclosion hormone immunoreactivity but little to no cGMP immunoreactivity in any ganglion. Controls showed strong cGMP immunoreactivity in all ganglia, and even greater reductions in eclosion hormone staining after ETH application. These results support previous suggestions that eclosion hormone is required for a positive feedback loop with ETH as well as onset of an inhibitory component, but also suggest that ETH stimulates eclosion hormone release at multiple spike initiation zones. The resultant up regulation of cGMP does not appear to be required for onset of ecdysis. A new model for ecdysis regulation is considered.

journal_name

Gen Comp Endocrinol

authors

Asuncion-Uchi M,El Shawa H,Martin T,Fuse M

doi

10.1016/j.ygcen.2009.08.008

subject

Has Abstract

pub_date

2010-03-01 00:00:00

pages

54-65

issue

1

eissn

0016-6480

issn

1095-6840

pii

S0016-6480(09)00331-1

journal_volume

166

pub_type

杂志文章
  • Arginine vasotocin and mesotocin levels in theca and granulosa layers of the ovary during the oviposition cycle in hens (Gallus domesticus).

    abstract::Neurohypophysial hormones in mammals are synthesized by the ovary and may influence reproductive function. Although arginine vasotocin (AVT) can induce premature oviposition in chickens, the gonadal content of AVT or mesotocin (MT) has not been examined in this species. Ovarian follicles were removed 2 hr before ovipo...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章

    doi:10.1016/0016-6480(90)90087-3

    authors: Saito N,Kinzler S,Koike TI

    更新日期:1990-07-01 00:00:00

  • Hormone studies in Myxine glutinosa: failure of calcitonin to influence urine flow or plasma and urine electrolyte balance.

    abstract::Hagfish, Myxine glutinosa, were used in a series of experiments to determine a possible role for calcitonin in plasma and urine electrolyte balance. Individual animals were anaesthetised and implanted with polythene cannulae for monitoring blood pressure and for infusion/injection. Urine was collected by manual palpat...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章

    doi:10.1016/0016-6480(88)90297-3

    authors: Wales NA

    更新日期:1988-07-01 00:00:00

  • In- and outdoor reproduction of first generation common sole Solea solea under a natural photothermal regime: Temporal progression of sexual maturation assessed by monitoring plasma steroids and gonadotropin mRNA expression.

    abstract::Reproduction of many temperate fishes is seasonal and maturation and spawning of gametes are under photothermal control. Reproductive success of first generation (G1) common sole Solea solea in captivity has been low. In this study, the sexual maturation status has been assessed during the prespawning months in G1 sol...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章

    doi:10.1016/j.ygcen.2014.12.004

    authors: Palstra AP,Blok MC,Kals J,Blom E,Tuinhof-Koelma N,Dirks RP,Forlenza M,Blonk RJ

    更新日期:2015-09-15 00:00:00

  • G-protein coupled estrogen receptor (GPER) inhibits final oocyte maturation in common carp, Cyprinus carpio.

    abstract::GPR-30, now named as GPER (G protein-coupled estrogen receptor) was first identified as an orphan receptor and subsequently shown to be required for estrogen-mediated signaling in certain cancer cells. Later studies demonstrated that GPER has the characteristics of a high affinity estrogen membrane receptor on Atlanti...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章

    doi:10.1016/j.ygcen.2014.11.011

    authors: Majumder S,Das S,Moulik SR,Mallick B,Pal P,Mukherjee D

    更新日期:2015-01-15 00:00:00

  • Seasonal patterns of sex steroids determined from feces in different social categories of Greylag geese (Anser anser).

    abstract::Seasonal patterns of fecal 17beta-OH-androgen, estrogen, and progesterone equivalents of male and female greylag geese (Anser anser) were analyzed in a flock of free-living geese. These were compared among social categories determined by pairbond status and breeding success. The annual cycle was divided into 13 phases...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章

    doi:10.1006/gcen.1998.7236

    authors: Hirschenhauser K,Möstl E,Kotrschal K

    更新日期:1999-04-01 00:00:00

  • Suppressed production of methyl farnesoid hormones yields developmental defects and lethality in Drosophila larvae.

    abstract::A long-unresolved question in the developmental biology of Drosophila melanogaster has been whether methyl farnesoid hormones secreted by the ring gland are necessary for larval maturation and metamorphosis. In this study, we have used RNAi techniques to inhibit 3-Hydroxy-3-Methylglutaryl CoA Reductase (HMGCR) express...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章

    doi:10.1016/j.ygcen.2009.07.006

    authors: Jones D,Jones G,Teal P,Hammac C,Messmer L,Osborne K,Belgacem YH,Martin JR

    更新日期:2010-01-15 00:00:00

  • Faecal cortisol metabolites in Bengal (Panthera tigris tigris) and Sumatran tigers (Panthera tigris sumatrae).

    abstract::The tiger (Panthera tigris) faces a great risk of extinction as its wild numbers have plummeted due to poaching and habitat destruction so ex-situ conservation programs are becoming ever more necessary. Reliable non-invasive biomarkers of the stress hormone (cortisol) are necessary for assessing the health and welfare...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章

    doi:10.1016/j.ygcen.2013.10.002

    authors: Narayan EJ,Parnell T,Clark G,Martin-Vegue P,Mucci A,Hero JM

    更新日期:2013-12-01 00:00:00

  • Influences of biological variables and geographic location on circulating concentrations of thyroid hormones in wild bottlenose dolphins (Tursiops truncatus).

    abstract::Thyroid hormones (TH) are key regulators of metabolism and development, yet our understanding of the variability in serum TH concentrations in free-ranging marine mammals is limited. Thus, we examined the interrelationships between TH and age, sex, reproductive status, geographic location, and ocean temperatures in wi...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章

    doi:10.1016/j.ygcen.2011.08.021

    authors: Fair PA,Montie E,Balthis L,Reif JS,Bossart GD

    更新日期:2011-11-01 00:00:00

  • Immunocytochemical localization, HPLC characterization, and seasonal dynamics of vasotocin in the brain, blood plasma and gonads of the catfish Heteropneustes fossilis.

    abstract::Immunocytochemical distribution and dynamics of vasotocin (VT) were studied in the air-breathing catfish Heteropneustes fossilis in relation to the reproductive cycle. Vasotocin was localized in the brain and ovary by streptavidin-biotin immunocytochemistry. The immunoreactivity was found throughout the hypothalamo-hy...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章

    doi:10.1016/j.ygcen.2008.09.003

    authors: Singh V,Joy KP

    更新日期:2008-11-01 00:00:00

  • Some new observations on the binding of thyroxine and triiodothyronine to plasma proteins and lipoproteins in the domestic fowl.

    abstract::In addition to the previously recognised thyroid hormone-binding proteins, three plasma components possibly involved in T3 and T4 transport have been identified in chicken plasma using gel filtration at pH 7.4. In mature female, though not male and immature female birds, a significant amount of T3 (11%) is bound to ve...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章

    doi:10.1016/0016-6480(85)90276-x

    authors: Mitchell MA,Stiles MS

    更新日期:1985-02-01 00:00:00

  • Molecular evolution of adiponectin in Carnivora and its mRNA expression in relation to hepatic lipidosis.

    abstract::Adiponectin is a novel adipocyte-derived hormone with low circulating concentrations and/or mRNA expression in obesity and non-alcoholic fatty liver disease (NAFLD). The adiponectin mRNA of several Carnivora species was sequenced to enable further gene expression studies in this clade with potential experimental speci...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章

    doi:10.1016/j.ygcen.2010.05.010

    authors: Nieminen P,Rouvinen-Watt K,Kapiainen S,Harris L,Mustonen AM

    更新日期:2010-09-15 00:00:00

  • Catecholamines, opioid peptides, and true opiates in the chromaffin cells of the eel: immunohistochemical evidence.

    abstract::An immunohistological analysis of the chromaffin cell system of the American eel revealed the presence of tyrosine hydroxylase (TH) and dopamine-beta-hydroxylase (DBH) in all cells. However, phenylethanolamine-N-methyltransferase (PNMT) was seen only in a fraction of the chromaffin cells. This suggests the presence of...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章

    doi:10.1016/0016-6480(90)90069-x

    authors: Hathaway CB,Epple A

    更新日期:1990-09-01 00:00:00

  • Ecdysteroid levels in Daphnia magna during a molt cycle: determination by radioimmunoassay (RIA) and liquid chromatography-mass spectrometry (LC-MS).

    abstract::Despite their abundance and their enormous significance for various ecological processes, endocrine systems of microcrustaceans have been poorly investigated. Here, we used a highly sensitive radioimmunoassay (RIA) to determine free and conjugated ecdysteroid levels in whole-body extracts of adult Daphnia magna during...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章

    doi:10.1016/j.ygcen.2006.11.015

    authors: Martin-Creuzburg D,Westerlund SA,Hoffmann KH

    更新日期:2007-03-01 00:00:00

  • Effects of time, photoperiod, and pinealectomy on ocular and plasma melatonin concentrations in the chick.

    abstract::Pinealectomized and intact chicks raised under cyclic (14L:10D) and noncyclic (OL;24L) conditions of environmental lighting were sampled 60 hr and 6 weeks after pinealectomy to evaluate the effects of pineal ablation on plasma and ocular melatonin concentrations. Little or no immunoreactive melatonin was present in pl...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章

    doi:10.1016/0016-6480(85)90114-5

    authors: Osol G,Schwartz B,Foss DC

    更新日期:1985-06-01 00:00:00

  • Gastroenteropancreatic hormones (insulin, glucagon, somatostatin, and multiple forms of PYY) from the pallid sturgeon, Scaphirhynchus albus (Acipenseriformes).

    abstract::Insulin, glucagon, somatostatin-14, and three structurally related molecular forms of peptide tyrosine-tyrosine (PYY) were isolated from an extract of the combined pancreas and gastrointestinal tract of the pallid sturgeon, Scaphirhynchus albus. Pallid sturgeon insulin was identical to insulin from the Russian sturgeo...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章

    doi:10.1006/gcen.2000.7571

    authors: Kim JB,Gadsbøll V,Whittaker J,Barton BA,Conlon JM

    更新日期:2000-12-01 00:00:00

  • Selective elimination/RNAi silencing of FMRF-related peptides and their receptors decreases the locomotor activity in Drosophila melanogaster.

    abstract::Five neuropeptide genes are classified in the FMRF-related (FaRP) group: the Fmrf, dromyosuppressin (Dms), drosulfakinin (Dsk), neuropeptide F (npf) and short neuropeptide F (sNPF) genes coding for 8, 1, 2, 1 and 4 peptides, respectively. In order to compare their effects on the locomotor activity of Drosophila adults...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章

    doi:10.1016/j.ygcen.2013.05.023

    authors: Kiss B,Szlanka T,Zvara Á,Žurovec M,Sery M,Kakaš Š,Ramasz B,Hegedűs Z,Lukacsovich T,Puskás L,Fónagy A,Kiss I

    更新日期:2013-09-15 00:00:00

  • Identification of 17,20β,21-trihydroxy-4-pregnen-3-one (20β-S) receptor binding and membrane progestin receptor alpha on southern flounder sperm (Paralichthys lethostigma) and their likely role in 20β-S stimulation of sperm hypermotility.

    abstract::The existence of direct progestin actions on teleost sperm to stimulate hypermotility is not widely acknowledged because it has only been demonstrated in members of the family Sciaenidae. In the present study, progestin stimulation of sperm hypermotility was investigated in a non-sciaenid, southern flounder, and the p...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章

    doi:10.1016/j.ygcen.2010.12.005

    authors: Tubbs C,Tan W,Shi B,Thomas P

    更新日期:2011-02-01 00:00:00

  • Characterization of channel catfish (Ictalurus punctatus) melanocortin-3 receptor reveals a potential network in regulation of energy homeostasis.

    abstract::The melanocortin-3 receptor (MC3R) is known to be involved in regulation of energy homeostasis, regulating feed efficiency and nutrient partitioning in mammals. Its physiological roles in non-mammalian vertebrates, especially economically important aquaculture species, are not well understood. Channel catfish (Ictalur...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章

    doi:10.1016/j.ygcen.2019.03.011

    authors: Yang LK,Zhang ZR,Wen HS,Tao YX

    更新日期:2019-06-01 00:00:00

  • Circannual and circadian variations in plasma levels of steroids (cortisol, estradiol-17 beta, estrone, and testosterone) correlated with the annual gonadal cycle in the catfish, Heteropneustes fossilis (Bloch).

    abstract::Circannual and circadian variations in plasma levels of steroids were estimated by radioimmunoassay in the female and male catfish, Heteropneustes fossilis, over two consecutive annual reproductive cycles. In the female catfish, testosterone (T), estradiol-17 beta (E2), and estrone (E1) were detectable in the plasma o...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章

    doi:10.1016/0016-6480(83)90221-6

    authors: Lamba VJ,Goswami SV,Sundararaj BI

    更新日期:1983-05-01 00:00:00

  • Effects of glucocorticoids on cartilage growth and response to IGF-I in the tilapia (Oreochromis mossambicus).

    abstract::To study the effects of glucocorticoids and IGF-I on the modulation of growth in the tilapia Oreochromis mossambicus, we employed an epiceratobranchial cartilage radioisotope incorporation assay, wherein radiolabeled sulfate and thymidine uptakes are measured in vitro to indicate proteoglycan synthesis and cell prolif...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章

    doi:10.1006/gcen.2000.7599

    authors: Datuin JP,Ng KP,Hayes TB,Bern HA

    更新日期:2001-03-01 00:00:00

  • Maturational steroids and gonadotropin in upstream migratory sockeye salmon.

    abstract::The circulating serum concentrations of various steroid hormones in mature sockeye salmon were measured at four different developmental stages in their upstream migration to spawn at Adams River in British Columbia, Canada. In females, a high level of estradiol-17 beta was found in fish at the first location, and it p...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章

    doi:10.1016/0016-6480(86)90098-5

    authors: Truscott B,Idler DR,So YP,Walsh JM

    更新日期:1986-04-01 00:00:00

  • Hormonal daily variation co-varies with immunity in captive male bullfrogs (Lithobates catesbeianus).

    abstract::Almost all physiological processes within the organism, including immune parameters and hormones, follow a circadian rhythm. These daily fluctuations are often observed in free-living organisms; however, little is known regarding hormonal and immune daily variations in anurans, particularly under laboratory conditions...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章

    doi:10.1016/j.ygcen.2020.113702

    authors: Titon SCM,Titon Junior B,Assis VR,Vasconcelos-Teixeira R,Garcia Neto PG,Lima AS,Ferreira LF,Fernandes PA,Gomes FR,Markus RP

    更新日期:2020-12-20 00:00:00

  • Identification and expression of nuclear receptor genes and ecdysteroid titers during nymphal development in the spider Agelena silvatica.

    abstract::Ecdysteroids play an essential role in the regulation of the molting processes of arthropods. Nuclear receptors of the spider Agelena silvatica that showed high homology with other arthropods especially in the functional domains were identified, two isoforms of ecdysone receptor (AsEcRA, AsEcRB), retinoid X receptor (...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章

    doi:10.1016/j.ygcen.2017.01.032

    authors: Honda Y,Ishiguro W,Ogihara MH,Kataoka H,Taylor D

    更新日期:2017-06-01 00:00:00

  • Isolation and structural characterisation of herring gull (Larus argentatus) pancreatic polypeptide.

    abstract::Pancreatic polypeptide (PP) has been isolated from pancreatic extracts of the herring gull (Larus argentatus) using a radioimmunoassay employing an antiserum (PP 221), generated to the conserved C-terminal hexapeptide amide of mammalian PP. Gel permeation and reverse-phase HPLC fractionation of crude pancreatic extrac...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章

    doi:10.1006/gcen.1994.1029

    authors: Barton CL,Shaw C,Halton DW,Thim L

    更新日期:1994-02-01 00:00:00

  • Abnormal growth hormone receptor gene expression in the sex-linked dwarf chicken.

    abstract::Sex-linked dwarfism is a recessive mutation that causes a reduction in body weight gain and long bone growth of chickens. We examined the effect of the dwarfing gene on body weight, hepatic GH-binding activity, and the structure and expression of the growth hormone receptor (GHR) gene in two different lines of sex-lin...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章

    doi:10.1016/0016-6480(92)90190-u

    authors: Burnside J,Liou SS,Zhong C,Cogburn LA

    更新日期:1992-10-01 00:00:00

  • Circannual changes in plasma aldosterone levels in Bufo japonicus formosus.

    abstract::Plasma aldosterone levels of Bufo japonicus formosus captured in their natural environment were determined each month throughout 1 year by radioimmunoassay. Low values were recorded in winter and spring, and the maximum occurred in July or August. High summer levels of aldosterone and increases in live weight were cor...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章

    doi:10.1016/0016-6480(84)90237-5

    authors: Jolivet-Jaudet G,Inoue M,Takada K,Ishii S

    更新日期:1984-01-01 00:00:00

  • Gonadal modulation of in vitro steroidogenic properties of dispersed adrenocortical cells from Sceloporus lizards.

    abstract::Effects of adrenal corticosteroids on reproductive and endocrine functions of the gonads are well known, but reciprocal effects of gonadal hormones on the hypothalamo-pituitary-adrenal (HPA) axis and on adrenocortical steroidogenesis in particular have received much less attention. We investigated effects of gonadecto...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章

    doi:10.1016/j.ygcen.2008.07.018

    authors: Carsia RV,McIlroy PJ,Cox RM,Barrett M,John-Alder HB

    更新日期:2008-09-01 00:00:00

  • Spontaneous intra-uterine growth restriction modulates the endocrine status and the developmental expression of genes in porcine fetal and neonatal adipose tissue.

    abstract::Low birth weight is correlated with low adiposity at birth, a phenotype that influences neonatal survival and later adiposity. A better understanding of events affecting the fetal adipose tissue development and its functionality around birth is thus needed. This study was undertaken to examine the impact of spontaneou...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章

    doi:10.1016/j.ygcen.2013.09.018

    authors: Gondret F,Père MC,Tacher S,Daré S,Trefeu C,Le Huërou-Luron I,Louveau I

    更新日期:2013-12-01 00:00:00

  • The influence of corticosterone and glucagon on metabolic recovery from exhaustive exercise in the desert iguana Dipsosaurus dorsalis.

    abstract::The skeletal muscles of ectothermic vertebrates possess an elevated glyconeogenic capacity that is responsible for a major portion of lactate removal and glycogen resynthesis following exercise. In lizards, changes in plasma hormone levels and the influence of differing hormone levels on muscle metabolism postexercise...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章

    doi:10.1006/gcen.1996.6866

    authors: Scholnick DA,Weinstein RB,Gleeson TT

    更新日期:1997-05-01 00:00:00

  • Urinary clearance of arginine vasotocin in Pekin ducks.

    abstract::To quantify the renal excretion of arginine vasotocin (AVT) in birds, synthetic AVT was infused into conscious Pekin ducks at rates of 0.03, 0.10, and 0.30 ng/kg/min for 30 min and the relationships between plasma and urine AVT concentrations were monitored by radioimmunoassay. As plasma AVT concentrations increased (...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章

    doi:10.1006/gcen.1995.1090

    authors: Gray DA

    更新日期:1995-07-01 00:00:00