Abstract：
:As an alternative to radioimmunoassays, a simple, highly sensitive and quick enzymeimmunoassay (EIA) for determination of testosterone in blood plasma of yaks on microtitreplates using second antibody coating technique and testosterone-horseradish peroxidase as a label has been developed for the first time. The wells of the microtitreplate were coated with affinity-purified goat immunoglobulin (antirabbit IgG) that binds the hormone specific antibody. The EIA was carried out directly in 20 mICROl of plasma after 1:10 dilution with assay buffer. The testosterone standard curve ranged from 0.2 to 200 pg/well. The sensitivity of the assay was 0.20 pg/well. Testosterone standard curve in buffer showed parallelism with serially diluted yak plasma containing high endogenous testosterone. Intra- and inter-assay coefficients of variation (CV) determined using pooled plasma was found 5.24 and 8.54%, respectively. Recovery of known concentrations of added testosterone in charcoal stripped plasma was linear (r=0.98). For biological validation of testosterone enzymeimmunoassay, the blood samples were collected from yak cows at -2h before and thereafter at 2h interval until 24h. after gonadotropin releasing hormone (GnRH) administration. There was a rapid increase (p<0.01) of luteinizing hormone (LH) and testosterone 2 and 6h after GnRH administration. Plasma testosterone concentration in normal adult yak bulls was found to be 0.52+/-0.09 ng/ml. In conclusion, the EIA developed in this study is simple, highly sensitive, valid and sufficiently reliable method for estimation of testosterone directly in yak plasma.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Sarkar M,Dutta Borah BK,Meyer HH,Prakash BSdoi
10.1016/j.ygcen.2008.07.007subject
Has Abstractpub_date
2008-09-15 00:00:00pages
240-4issue
3eissn
0016-6480issn
1095-6840pii
S0016-6480(08)00273-6journal_volume
158pub_type
杂志文章abstract：:In teleosts, lungfish, amphibians, and a reptile, Amphibolurus nuchalis, hormonal stimulation of hepatic glycogenolysis is mediated by a rise in intracellular cyclic AMP concentration. In mammals, by contrast, the inositol trisphosphate/Ca2+/diacylglycerol signal transduction pathways are also involved. The present st...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(92)90200-4
更新日期：1992-10-01 00:00:00
abstract：:Differential transfer of maternal testosterone (T) into egg yolk provides a means of adjusting an offspring's phenotype to ambient environmental conditions. While the environmental and genetic driven variability in yolk T levels is widely described, the underlying mechanisms are poorly understood. Here, we investigate...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2016.08.016
更新日期：2016-10-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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更新日期：1988-04-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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更新日期：2016-04-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2005.12.010
更新日期：2006-06-01 00:00:00
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journal_title：General and comparative endocrinology
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更新日期：2015-11-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2014.08.014
更新日期：2014-11-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2005.01.006
更新日期：2005-05-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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更新日期：1989-08-01 00:00:00
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journal_title：General and comparative endocrinology
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更新日期：2012-01-15 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2008.02.007
更新日期：2008-05-15 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1997.7028
更新日期：1998-02-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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更新日期：2019-09-15 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(91)90301-l
更新日期：1991-04-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(89)90138-x
更新日期：1989-12-01 00:00:00
abstract：:The skin of Xenopus laevis, because of its high concentrations of amidated peptides, is an excellent model for the study of neuropeptide biosynthesis. In this paper we determined levels of the mRNAs which encode cerulin and characterized the enzymatic activity which likely produces the carboxyl terminal alpha-amide mo...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(87)90206-1
更新日期：1987-07-01 00:00:00
abstract：:1,25-dihydroxyvitamin D3 (1,25(OH)2D3), the hormonally active metabolite of vitamin D3 interacts with its nuclear/cytosolic receptor to induce biological responses in target tissues. Naked mole rats appear to be naturally deficient in vitamin D. The questions arise whether these animals possess the 1,25(OH)2D3 recepto...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1993.1089
更新日期：1993-06-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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更新日期：1990-08-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2011.05.010
更新日期：2011-08-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(88)90030-5
更新日期：1988-03-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1999.7363
更新日期：1999-11-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章,评审
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更新日期：2019-08-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章,评审
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更新日期：2006-05-15 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(84)90186-2
更新日期：1984-05-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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更新日期：1988-09-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1994.1160
更新日期：1994-10-01 00:00:00
abstract：:Urinary corticosterone metabolite responses to capture have recently been shown for the first time in amphibians, and in the present study urinary corticosterone metabolite responses to capture and to confinement in captivity were measured in adult cane toads (Rhinella marina) in Queensland, Australia. An adrenocortic...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2011.06.015
更新日期：2011-09-01 00:00:00
abstract：:Dopamine (DA) has a pivotal role in avian prolactin (PRL) secretion, acting centrally through D(1) DA receptors to stimulate PRL secretion by operating through vasoactive intestinal peptide (VIP). DA also inhibits PRL secretion by activating D(2) DA receptors at the pituitary level. This study was designed to investig...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2008.08.002
更新日期：2008-10-01 00:00:00
abstract：:Growth hormone (GH) and insulin-like growth factor-I (IGF-I) play major roles in the endocrine regulation of fish growth, but their interdependency and mode of action has not been well elucidated. The GH-IGF-I system is essential for normal vertebral growth in mouse, but this has not been studied in fish. To study the...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2004.12.005
更新日期：2005-05-15 00:00:00
