Abstract：
:We studied effects of in vitro conditions on the steady-state distribution and exchange of thyroid hormones (TH) between red blood cells (RBC) and plasma of rainbow trout. At steady state at 12 degrees C the RBC contained 5-11% of L-thyroxine (T4), 14-23% of 3,5,3'-triiodo-L-thyronine (T3), and 23-24% of 3,3',5'-triiodo-L-thyronine (rT3) present in whole blood. The steady-state distribution was (i) higher in immature than in mature trout for T3, (ii) increased by incubation temperature from 0 to 22 degrees C for both T4 and T3, (iii) unaltered by blood T4 or T3 concentration (0-40 ng/ml), and (iv) increased by O2 gassing and decreased by N2 gassing for T4 and rT3, but negligibly for T3. The exchange between RBC and plasma was more rapid for T3 (50% of maximal influx or efflux at 30-40 s) than rT3 (14 min) than T4 (30 min). Efflux of T4 and T3 was greatly reduced in the absence of plasma protein. Incubation with 10% bovine serum albumin extracted > 98% of labeled T4 and T3 from RBC. We conclude that for trout (i) steady-state distribution and exchange kinetics between RBC and plasma differ greatly for T4, T3, and rT3 and vary with the in vitro conditions, (ii) almost all TH in RBC are reversibly bound to intracellular sites, (iii) efflux is strongly influenced by plasma binding sites, (iv) T3 exchange is rapid and may allow T3 access to RBC TH receptors, buffer plasma T3 levels, or aid T3 delivery to tissues, (v) T4 exchange is slow and this may prevent oxygenation state from altering T4 uptake into RBC, and (vi) rT3 uptake into RBC may contribute to low rT3 levels in trout plasma.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
McLeese J,Waytiuk A,Eales JGdoi
10.1006/gcen.1997.7028subject
Has Abstractpub_date
1998-02-01 00:00:00pages
259-68issue
2eissn
0016-6480issn
1095-6840pii
S0016-6480(97)97028-3journal_volume
109pub_type
杂志文章abstract：:Gonad-stimulating substance (GSS) in starfish is the only known invertebrate peptide hormone responsible for final gamete maturation, rendering it functionally analogous to gonadotropins in vertebrates. In breeding season (stage V), GSS stimulates oocyte maturation to induce 1-methyladenine (1-MeAde) by ovarian follic...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2014.06.003
更新日期：2014-09-01 00:00:00
abstract：:Full-length complementary deoxyribonucleic acid as well as genomic sequences encoding for two gastrointestinal appetite-related peptides, ghrelin and for gastrin-releasing peptide (GRP) were cloned from Atlantic cod (Gadus morhua) stomach using reverse transcription and rapid amplification of complementary deoxyribonu...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2008.12.004
更新日期：2009-02-01 00:00:00
abstract：:Adiponectin is a novel adipocyte-derived hormone with low circulating concentrations and/or mRNA expression in obesity and non-alcoholic fatty liver disease (NAFLD). The adiponectin mRNA of several Carnivora species was sequenced to enable further gene expression studies in this clade with potential experimental speci...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2010.05.010
更新日期：2010-09-15 00:00:00
abstract：:The existence of direct progestin actions on teleost sperm to stimulate hypermotility is not widely acknowledged because it has only been demonstrated in members of the family Sciaenidae. In the present study, progestin stimulation of sperm hypermotility was investigated in a non-sciaenid, southern flounder, and the p...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2010.12.005
更新日期：2011-02-01 00:00:00
abstract：:The chromatographic profile of ecdysteroids (Ecds) from the midgut gland (MG) of juvenile female lobsters, Homarus americanus, was examined using high-performance liquid chromatography (HPLC) and radioimmunoassay (RIA) over four stages of the molt cycle. Upon initial examination, highly polar Ecd conjugates appeared t...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(92)90013-a
更新日期：1992-02-01 00:00:00
abstract：:Using purified thyroglobulin (TG) of Rana catesbeiana and its antisera, a specific double-antibody radioimmunoassay (RIA) was developed. Serum TG content was measured in tadpoles, froglets, and adult frogs by RIA. Serum TG increased during pre- and prometamorphosis and reached a peak of 476.9 +/- 29.1 ng/ml at the cli...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1994.1061
更新日期：1994-04-01 00:00:00
abstract：:European silver eels are thought to undergo sexual maturation during their oceanic reproductive migration from the European continent to their spawning area in the Sargasso Sea. Tracking data and various anatomical and physiological features suggest that silver eels migrate in deep sea, leading us to hypothesise that ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2007.01.009
更新日期：2007-08-01 00:00:00
abstract：:The photoperiodic control of puberty in birds was studied using hybrid red-legged partridges (Alectoris graeca chukar). In the wild partridges first breed at about 10 months of age. Males and females were held from hatch on short (8L:16D) daylengths and then at 3, 4 1/2, 6, 7 1/2, 9, and 12 weeks of age groups were tr...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(88)90290-0
更新日期：1988-07-01 00:00:00
abstract：:The importance of late winter pairing activity on the neuroendocrinology of wild waterfowl is unknown. In this study, we examined the sex-related differences in the roles of late winter pairing activity and seasonal influences on neuroendocrine and reproductive physiology in both male and female mallards. Our main goa...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.2000.7469
更新日期：2000-05-01 00:00:00
abstract：:Dopamine (DA) was infused into the third ventricle of anesthetized laying turkey hens at various concentrations to determine its effect on both basal prolactin (PRL) levels and ongoing electrically induced PRL secretion. The infusion of DA at rates of 1.0 or 10.0 nmol/min resulted in dose dependent increases in plasma...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1995.1049
更新日期：1995-04-01 00:00:00
abstract：:The initiation of sexual maturation and spermatogenesis are complex processes that require the highly coordinated regulation of a number of key genes. The endocrine system plays crucial roles in these processes, but the precise mechanisms involved in sexual maturation of fish are poorly understood. We investigated the...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2008.08.008
更新日期：2008-11-01 00:00:00
abstract：:Pituitary cells were prepared by enzymatic dispersion and incubated in vitro. To observe the effect of gonadotropin releasing hormone (GnRH) and Ca2+ on the murrel pituitary cyclic 3',5'-AMP (cAMP), cells were dispersed by 0.3% collagenase plus 0.05% trypsin in Earle's minimum essential medium without Ca2+ and a consi...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1995.1035
更新日期：1995-03-01 00:00:00
abstract：:Methallibure treatment (1 mg fed on alternate days to a female and a male kept in 1 liter of 25% seawater) started from 1 or 7 days postpartum (early gestation) inhibited gestation in the wild guppy, Poecilia reticulata. A few of the fish treated from 7 days postpartum (2/9) did give birth, but the young either were d...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(85)90329-6
更新日期：1985-11-01 00:00:00
abstract：:Methyl farnesoate (MF) stimulation of ecdysteroid secretion by Y-organs of the crab, Cancer magister, is demonstrated. Isolated Y-organs that were incubated with a mandibular organ (MO) secreted significantly higher levels of ecdysteroids into the medium when compared to those incubated in the absence of a MO. MOs sec...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1993.1050
更新日期：1993-03-01 00:00:00
abstract：:The thyroid stimulating hormone receptor gene (TSHR) has been suggested to be a "domestication locus" in the chicken. A strong selective sweep over TSHR in domestic breeds together with significant effects of a mutation in the gene on several domestication related traits, indicate that the gene has been important for ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2016.02.010
更新日期：2016-03-01 00:00:00
abstract：:The administration of arginine vasotocin (AVT) to gravid snapping turtles with steroidogenically active corpora lutea and high plasma progesterone concentration (1480 +/- 155 pg/ml) did not trigger oviposition, whereas 12 days after ovulation when luteolysis occurred and plasma progesterone concentration was low (570 ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(88)90052-4
更新日期：1988-01-01 00:00:00
abstract：:Using frog ovarian follicles in vitro we assessed the role of the theca/epithelium (THEP) layer and granulosa cells in the production of steroids. The THEP layer and granulosa cell-enclosed oocytes (GCEOs) were separated from ovarian follicles of the frog, Rana nigromaculata, by microdissection. Intact follicles (IFs)...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1994.1077
更新日期：1994-05-01 00:00:00
abstract：:To study the expression of four estrogen receptor genes (erα1, erα2, erβ1, erβ2) of female rainbow trout (Oncorhynchus mykiss) during first ovarian development, trouts were sampled from different ovarian stages. Serum E2 (estradiol) was measured by ELISA and estrogen receptors mRNA expression were examined by qRT-PCR....
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2017.10.001
更新日期：2018-04-01 00:00:00
abstract：:Previous data have shown that regional differences in the presence of anterior pituitary luteinizing hormone (LH) generally correlate with the comparable disparities in distribution of gonadotropes throughout the gland. In female rats, the differences are apparent over the estrous cycle, but are more prominent during ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2008.02.015
更新日期：2008-05-01 00:00:00
abstract：:Effects of adrenal corticosteroids on reproductive and endocrine functions of the gonads are well known, but reciprocal effects of gonadal hormones on the hypothalamo-pituitary-adrenal (HPA) axis and on adrenocortical steroidogenesis in particular have received much less attention. We investigated effects of gonadecto...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2008.07.018
更新日期：2008-09-01 00:00:00
abstract：:Due to its unique organization, the teleost pituitary is an ideal model in which to investigate the relationship of the nervous system with the pituitary endocrine cells. A light microscope immunocytochemical study of the sea bass pituitary revealed six different neuropeptides in nerve fibers which projected into the ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(88)90200-6
更新日期：1988-11-01 00:00:00
abstract：:The concentrations of norepinephrine in hypothalamus and norepinephrine and epinephrine in head kidney were significantly decreased in treated tilapia (Oreochromis aureus) during the time course of cold exposure (12 degrees) as compared to the control (25 degrees). The elevation of norepinephrine and epinephrine in pl...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.2001.7772
更新日期：2002-03-01 00:00:00
abstract：:Hormones play key, functional roles in mediating the tradeoff between survival and reproduction. Glucocorticoid hormones can inhibit reproduction and improve chances of survival during periods of stress. However, glucocorticoid hormones are, at times, also associated with successfully engaging in energetically costly ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2014.08.008
更新日期：2014-11-01 00:00:00
abstract：:The effects of PGE1, PGE2, PGF2 alpha (2 micrograms/ml), and forskolin (10 microM) on follicle contraction of brook trout (salvelinus fontinalis) were studied by measuring the weight loss of punctured follicles during in vitro incubation. PGE1, PGE2, and forskolin significantly inhibited spontaneous contraction of fol...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(92)90238-f
更新日期：1992-12-01 00:00:00
abstract：:Gonadotropin-releasing hormone (GnRH) was detected in the brains of passerine birds, a recently evolved and diverse avian group. The molecular forms of GnRH in two species of birds under breeding conditions were deduced using methods of HPLC and immunology. The brain extracts of song sparrows (Melospiza melodia) conta...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(88)90024-x
更新日期：1988-03-01 00:00:00
abstract：:High-resistance epithelia derived from amphibian sources such as frog skin, toad urinary bladder, and the A6 Xenopus laevis kidney cell line have been widely used to elucidate the underlying mechanisms involved in the regulation of vectorial ion transport. More recently, the isolation of high-resistance mammalian cell...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2005.11.010
更新日期：2006-05-15 00:00:00
abstract：:The effects of epinephrine (E), norepinephrine (NE), phenylephrine (PHE), and isoproterenol (ISO) were studied in vivo on the blood pressure and heart rate of the conscious American bullfrog, Rana catesbeiana. Bullfrogs were chronically cannulated with a T cannula in the right sciatic artery. In warm-acclimated (22 de...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(85)90401-0
更新日期：1985-09-01 00:00:00
abstract：:The hybrids of carp (Cyprinus carpio) and goldfish (Carassius auratus) collected from the lower Great Lakes between 1978 and 1981 exhibited epizootics of gonadal neoplasm which were rare in the parental species. The pituitary glands of hybrids were, on average, 2.5 times larger than that of carp of similar body size. ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(88)90017-2
更新日期：1988-02-01 00:00:00
abstract：:In seasonally breeding, photoperiodic birds, the development of photorefractoriness is associated with decreased brain expression of gonadotropin-releasing hormone-like immunoreactivity (GnRH-li ir) and increased expression of vasoactive intestinal polypeptide-like immunoreactivity (VIP-li ir). Dissipation of photoref...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1999.7361
更新日期：2000-01-01 00:00:00
abstract：:Using DNA-cellulose affinity chromatography, an estrogen-binding component having the properties of a classical estrogen receptor was characterized from testicular cytosol of the freshwater turtle, Chrysemys picta. This putative cytoplasmic receptor exhibited high affinity (Kd = 7.0 X 10(-10) M), low capacity (1-4 fmo...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(83)90111-9
更新日期：1983-11-01 00:00:00
