Abstract：
:The objective of this study was to examine if RU486, a corticosteroid antagonist, modified hepatocyte metabolism and blocked the metabolic effects of exogenously administered cortisol in rainbow trout (Oncorhynchus mykiss). The fish were given a single intraperitoneal implant of either coconut oil alone or coconut oil containing RU486 (100 mg.kg-1), cortisol (100 mg.kg-1), or a combination of RU486 and cortisol and were sampled 7 days postimplantation. The RU486 implants had no effect on plasma cortisol and glucose concentrations, hepatocyte glycogen content, and total glucose production, but increased in vitro hepatocyte glycogen breakdown. Cortisol implantation had no effect on plasma glucose concentration, hepatocyte glycogen content, glycogen breakdown, or total glucose production, while it significantly increased alanine oxidation and gluconeogenesis in rainbow trout hepatocytes. Hepatocyte responsiveness to epinephrine and glucagon stimulation of total glucose production was not modified by either RU486 or cortisol treatment, whereas the insulin response on total glucose production was decreased with cortisol. RU486 treatment modified hepatocyte glycogen metabolism and blocked the cortisol-induced increases in alanine gluconeogenesis and glycogen mobilization for endogenous use by the hepatocytes. These results indicate that cortisol enhances the metabolic potential of hepatocytes, thereby adapting the fish to cope with stress. This study provides further validation of RU486 as a tool for studying metabolic actions of cortisol in fish.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Vijayan MM,Reddy PK,Leatherland JF,Moon TWdoi
10.1006/gcen.1994.1160subject
Has Abstractpub_date
1994-10-01 00:00:00pages
75-84issue
1eissn
0016-6480issn
1095-6840pii
S0016-6480(84)71160-9journal_volume
96pub_type
杂志文章abstract：:In flatfishes with asynchronous and semicystic spermatogenesis, such as the Senegalese sole (Solea senegalensis), the specific roles of the pituitary gonadotropins during germ cell development, particularly of the follicle-stimulating hormone (Fsh), are still largely unknown in part due to the lack of homologous immun...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2014.10.009
更新日期：2015-09-15 00:00:00
abstract：:The vertebrate oxytocin and vasopressin receptors form a family of G-protein-coupled receptors (GPCRs) that mediate a large variety of functions, including social behavior and the regulation of blood pressure, water balance and reproduction. In mammals four family members have been identified, three of which respond t...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2011.10.011
更新日期：2012-01-01 00:00:00
abstract：:Factors potentially regulating hepatic growth hormone (GH) receptors in coho salmon (Oncorhynchus kisutch) have been investigated. From December to June of the first year, relative changes in hepatic 125I-sGH binding and 35SO4 incorporation by ceratobranchial cartilage were similar. Stunted salmon, which in seawater h...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(92)90256-j
更新日期：1992-11-01 00:00:00
abstract：:Behavioral, biochemical, and electrophysiological studies suggest that the trihydroxylated progestin steroid, 4-pregnen-17,20beta,21-triol-3-one (20beta-S) stimulates oocyte maturation and pheromone release in the Eurasian ruffe, a freshwater percid fish. Behavioral observations found that female ruffe undergoing oocy...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2004.05.014
更新日期：2004-10-01 00:00:00
abstract：:European silver eels are thought to undergo sexual maturation during their oceanic reproductive migration from the European continent to their spawning area in the Sargasso Sea. Tracking data and various anatomical and physiological features suggest that silver eels migrate in deep sea, leading us to hypothesise that ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2007.01.009
更新日期：2007-08-01 00:00:00
abstract：:To better understand the role(s) of progesterone in fish spermatogenesis, we cloned the nuclear progesterone receptor (Pgr) of Atlantic cod. The open-reading frame of the cod pgr consists of 2076 bp, coding for a 691-amino acids-long protein that shows the highest similarity with other piscine Pgr proteins. Functional...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2012.07.022
更新日期：2012-10-01 00:00:00
abstract：:Using antibodies to the amino-terminal region of human parathyroid hormone-related protein (PTHrP) we have demonstrated PTHrP immunoreactivity in pituitaries and plasma of the sea bream (Sparus aurata). Pituitary cells at two distinct locations contained immunodetectable PTHrP; an anterior group in the rostral pars di...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1993.1156
更新日期：1993-11-01 00:00:00
abstract：:The possible involvement of calcium in the regulation of steroidogenesis in the goldfish was investigated using preovulatory ovarian follicles incubated in vitro. Incubation of follicles in media deficient in calcium impaired testosterone production in response to human chorionic gonadotropin (hCG) in both the presenc...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(91)90011-t
更新日期：1991-02-01 00:00:00
abstract：:Aromatase is a key regulator of circulating testosterone (T) and 17-β-oestradiol (E2), two steroids which are critical to the development, maintenance and function of reproductive tissues. The role of aromatase in sexual differentiation in oviparous (egg-laying) reptiles is well understood, yet has never been explored...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2014.02.020
更新日期：2014-05-01 00:00:00
abstract：:Serum levels of 17 beta-hydroxy-4-androstene-3,11-dione (11-KT) and testosterone in the protogynous black sea bass, Centropristis striatus fluctuate annually, correlated with the breeding season. Although serum 11-KT levels in both males and females exhibit seasonality, serum estradiol-17 beta concentrations cycle ann...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(91)90297-j
更新日期：1991-04-01 00:00:00
abstract：:Serum prolactin (PRL) and luteinizing hormone (LH) levels from mature female turkeys were determined following ovariectomy and daily subcutaneous injections for 4 or 9 days of 0.002-2.0 mg/kg estradiol benzoate (EB), 0.05-2.0 mg/kg progesterone (P), or their combinations. Serum PRL levels were increased (P less than 0...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(83)90159-4
更新日期：1983-10-01 00:00:00
abstract：:Birds use both the corticosterone stress response and immune system to meet physiological challenges during exposure to adverse climatic conditions. To assess the stress level and immune response of the Asian Great Bustard during conditions of severe winter weather, we measured fecal corticosterone (CORT) and Immunogl...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2018.02.014
更新日期：2018-05-15 00:00:00
abstract：:The conversion in vitro of cholesterol (Ch) to nonpolar metabolites and ecdysteroids was studied in Y-organs of the xanthid crab, Menippe mercenaria. In one set of experiments, Y-organs were prelabeled in vivo by injecting crabs with 100 microCi of [3H]Ch, and halved glands were then incubated for 24 and 48 hr in the ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(92)90255-i
更新日期：1992-11-01 00:00:00
abstract：:Insulin-like growth factor-1 (IGF-1) is a member of the vertebrate insulin/insulin-like growth factor/relaxin gene family necessary for growth, reproduction, and survival at both the cellular and organismal level. Its sequence, protein structure, and function have been characterized in mammals, birds, and fish; howeve...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2012.04.022
更新日期：2012-08-01 00:00:00
abstract：:The titer of juvenile hormone (JH), which has wide ranging physiological effects in insects, is regulated in part by JH esterase (JHE). We show that overexpression in Drosophila melanogaster of the JHE binding protein, DmP29 results in a series of apparent anti-JH effects. We hypothesize that DmP29 functions in transp...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2008.01.006
更新日期：2008-03-01 00:00:00
abstract：:In Seriola species, exposure to a long photoperiod regime is known to induce ovarian development. This study examined photoperiodic effects on pituitary gene expression and plasma levels of follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh) in previtellogenic greater amberjack (Seriola dumerili). The fish...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2018.09.007
更新日期：2018-12-01 00:00:00
abstract：:In the teleost brain, radial glial cells (RGCs) are the main macroglia and are stem-like progenitors that express key steroidogenic enzymes, including the estrogen-synthesizing enzyme, aromatase B (cyp19a1b). As a result, RGCs are integral to neurogenesis and neurosteroidogenesis, however little is known about the reg...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2017.04.014
更新日期：2018-02-01 00:00:00
abstract：:Potamopyrgus antipodarum is a promising test organism that is often used in ecotoxicology, both in laboratory and field exposures. As no data are available on the physiological variation range of its life-traits and the biomarkers it uses, we studied the variation of fecundity, steroid levels and energy reserves over ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2011.03.011
更新日期：2011-06-01 00:00:00
abstract：:In the present study, four full-length cDNAs of somatostatin receptor (sstr) were cloned from the forebrain and pituitary of red-spotted grouper. The four full-length cDNAs were designated 2292, 1522, 1873 and 1789 bp and identified as sstr1, sstr2, sstr3, and sstr5 by BLAST analysis; the corresponding sizes of the op...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2019.01.007
更新日期：2019-04-01 00:00:00
abstract：:Unlike mammals, the goldfish is unique in having dopamine (DA) D1 receptors in the anterior pituitary. In this species, DA stimulates growth hormone (GH) release via D1 receptors coupled to the cAMP-dependent pathway. To further examine the postreceptor mechanisms of this novel pituitary DA D1 system, the role of extr...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1994.1088
更新日期：1994-06-01 00:00:00
abstract：:The present work studied the PGF2 alpha and 17 beta-estradiol plasma levels during ovulation, and the in vitro effects of mammalian gonadotropin-releasing hormone (mGnRH) on ovarian and oviductal production of prostaglandin F2 alpha (PGF2 alpha) and 17 beta-estradiol during four different stages of the annual sexual c...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(92)90018-f
更新日期：1992-08-01 00:00:00
abstract：:The thyroid stimulating hormone receptor gene (TSHR) has been suggested to be a "domestication locus" in the chicken. A strong selective sweep over TSHR in domestic breeds together with significant effects of a mutation in the gene on several domestication related traits, indicate that the gene has been important for ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2016.02.010
更新日期：2016-03-01 00:00:00
abstract：:Myopathies (Woody Breast (WB) and White Striping (WS)) of broiler chickens have been correlated with fast growth. Recent studies reported that localized hypoxia and metabolic impairment may involve in these myopathies of birds. In order to better understand the stress response mechanisms affecting myopathies of broile...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2020.113526
更新日期：2020-09-01 00:00:00
abstract：:The reproductive biology of the Indian major carp Cirrhinus mrigala is tightly synchronized with the seasonal changes in the environment. While the ovaries show growth from February through June, the fish spawn in July-August to coincide with the monsoon; thereafter the fish pass into the postspawning and resting phas...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/s0016-6480(03)00190-4
更新日期：2003-10-01 00:00:00
abstract：:Corticosterone, at baseline and moderately elevated levels, is thought to regulate energy mobilization during the predictable life-history cycle. In birds, corticosterone is known to be moderately elevated during migration, and some experiments on captive, but migratory active birds have shown that exogenous corticost...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2013.02.042
更新日期：2013-06-01 00:00:00
abstract：:The insulin receptors of frog brain and liver show features typical of other insulin receptors with regard to affinity and specificity of binding to insulins and proinsulin, solubility in Triton X-100, binding to and elution from wheat germ agglutinin, and insulin-sensitive tyrosine kinase activity. Likewise, the brai...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(87)90026-8
更新日期：1987-11-01 00:00:00
abstract：:In recent years, invertebrate peptides have been identified which share substantial homologies with vertebrate insulin and insulin-like growth factors (IGFs), indicating a high degree of conservation of insulin/IGF systems through animal evolution. In a previous study, we provided evidence for the presence of IGF-I-li...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1996.6824
更新日期：1997-03-01 00:00:00
abstract：:Changes in testis size, histological status, and plasma levels of testosterone were monitored for males of three species of Western Australian desert frogs, Cyclorana maini, Cyclorana platycephala, and Neobatrachus sutor during aestivation. The frogs were induced to burrow and form cocoons soon after their capture and...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2003.12.005
更新日期：2004-03-01 00:00:00
abstract：:In vertebrates, the ontogeny of several crucial organismal systems is known to occur early in life. Developmental conditions can ultimately have important consequences on adult fitness by affecting individual phenotype. These developmental effects are thought to be primarily mediated by endocrine systems, and especial...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2019.02.021
更新日期：2019-05-15 00:00:00
abstract：:Over the last decades, the corticosterone stress response has been suggested as a major physiological tool to understand what strategy an individual might adopt in response to environmental perturbations. More recently, another hormone related to parental care--prolactin--has been suggested as a complementary tool to ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2012.10.008
更新日期：2013-02-01 00:00:00
