Abstract：
:To better understand the role(s) of progesterone in fish spermatogenesis, we cloned the nuclear progesterone receptor (Pgr) of Atlantic cod. The open-reading frame of the cod pgr consists of 2076 bp, coding for a 691-amino acids-long protein that shows the highest similarity with other piscine Pgr proteins. Functional characterization of the receptor expressed in mammalian cells revealed that the cod Pgr exhibited progesterone-specific, dose-dependent induction of reporter gene expression, with 17α,20β-dihydroxy-4-pregnen-3-one (DHP), a typical piscine progesterone, showing the highest potency in activating the receptor. During ontogenesis, the pgr mRNA was undetectable in embryo's 24 h after fertilization, but became detectable 4 days after fertilization. During the larval stage, the expression levels increased steadily with the development of the larvae. In adult fish, pgr was predominantly expressed in gonads of both sexes. During the onset of puberty, testicular pgr transcript levels started to increase during rapid spermatogonial proliferation, and peaked when spermiation started. In situ hybridization studies using testis tissue during the rapid growth phase containing all germ cell stages indicated that in cod, pgr mRNA is predominantly located in Sertoli cells that are in contact with proliferating spermatogonia. Taken together, our data suggests that the Pgr is involved in mediating progestagen stimulation of the mitotic expansion of spermatogonia, and in processes associated with the spermiation/spawning period in Atlantic cod.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Chen SX,Almeida FF,Andersson E,Taranger GL,Schmidt R,Schulz RW,Bogerd Jdoi
10.1016/j.ygcen.2012.07.022subject
Has Abstractpub_date
2012-10-01 00:00:00pages
71-7issue
1eissn
0016-6480issn
1095-6840pii
S0016-6480(12)00310-3journal_volume
179pub_type
杂志文章abstract：:Birds respond to environmental changes by modulating their levels of plasma corticosterone, a stress hormone produced by the adrenal glands. Baseline levels of corticosterone and the magnitude of adrenocortical response to acute stress are known to vary seasonally. Photoperiod is one of several potential factors which...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.2002.7798
更新日期：2002-04-01 00:00:00
abstract：:Plasma glucocorticoid (CORT) levels collected within 3min of capture are commonly believed to reflect pre-stressor, baseline CORT levels. Differences in these "baseline" values are often interpreted as reflecting differences in health, or the amount of social and environmental stress recently experienced by an individ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2017.01.028
更新日期：2017-06-01 00:00:00
abstract：:Arginine vasotocin (AVT) and oxytocin (OXY), in the dose range of 2 X 10(-13)-2 X 10(-8) mol, were found to be potent vasoconstrictors of the arterioarterial pathway of isolated perfused eel gills. AVT was significantly (P less than 0.05) more potent than OXY, as indicated by the results of both dose- and concentratio...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(86)90028-6
更新日期：1986-10-01 00:00:00
abstract：:In the genetic sex determination of vertebrates, the gonadal sex depends on the combination of sex chromosomes that a zygote possesses. Despite the discovery of the sex-determining gene (SRY/Sry) in mammals in 1990s, the sex-determining gene in non-mammalian vertebrates remained an enigma for over a decade. In most ma...
journal_title：General and comparative endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.ygcen.2015.10.004
更新日期：2016-12-01 00:00:00
abstract：:Glucagon and peptide of the neuropeptide Y (NPY) family immunoreactivities were studied in the gut of sea bass (Dicentrarchus labrax) using antisera against bovine/porcine glucagon, porcine glucagon, glicentin (10-30), bovine pancreatic polypeptide (PP), peptide tyrosine tyrosine (PYY), salmon PYY (sPYY), and NPY. Glu...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1998.7119
更新日期：1998-10-01 00:00:00
abstract：:Early maternal effects in the form of substances accumulated in the egg, such as carotenoids and hormones, can be physiologically relevant for a good development of offspring. It has been found in different species that testosterone (T) can be beneficial to offspring by increasing growth rate, but detrimental by reduc...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2007.12.010
更新日期：2008-04-01 00:00:00
abstract：:Oxidative stress impaired sperm function might lead to infertility. The objective of this study was to evaluate the effects of altered thyroid hormone levels on regulation of mitochondrial glutathione redox status and its dependent antioxidant defense system in adult rat testis and their correlation with testicular fu...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2010.07.014
更新日期：2010-10-01 00:00:00
abstract：:This study directly tested the hypothesis that 17 alpha,20 beta-dihydroxy-4-pregnen-3-one (17,20 beta-P) is a goldfish preovulatory pheromone (pheromone released at peak levels during oocyte final maturation) which increases blood gonadotropin (GtH) and milt volume in males. During spontaneous ovulation, GtH and 17,20...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(89)90008-7
更新日期：1989-07-01 00:00:00
abstract：:This study was designed to examine the relationship among ovulation rate, inhibin alpha-subunit gene expression, and plasma immunoreactive inhibin levels between hens laying at a high ovulation rate and hens laying at a low ovulation rate. A porcine inhibin alpha-subunit cDNA was used to examine the expression of inhi...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1993.1103
更新日期：1993-07-01 00:00:00
abstract：:We studied the hepatic in vitro deconjugation and deiodination of glucuronide (G) and sulfate (S) conjugates of the thyroid hormones (TH) thyroxine (T(4)), 3,5,3'-triiodothyronine (T(3)), and 3,3', 5'-triiodothyronine (rT(3)) in trout. These conversions have not been studied in nonmammals. Deconjugation of T(4)G, T(3)...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1999.7326
更新日期：1999-09-01 00:00:00
abstract：:A conglomerate of 8-12 neurons in the medulla externa of the crayfish eyestalk was explored in their reaction to a polyclonal antibody against the tyrosinated octapeptide Red Pigment Concentrating Hormone (Tyr-RPCH). These are large neurons with diameters within a range of 33-43 microns and they were all positively st...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1994.1143
更新日期：1994-09-01 00:00:00
abstract：:Aromatase is a key regulator of circulating testosterone (T) and 17-β-oestradiol (E2), two steroids which are critical to the development, maintenance and function of reproductive tissues. The role of aromatase in sexual differentiation in oviparous (egg-laying) reptiles is well understood, yet has never been explored...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2014.02.020
更新日期：2014-05-01 00:00:00
abstract：:Connective tissue growth factor (CTGF) is a cysteine-rich, matrix-associated heparin-binding protein that is important in many cell types as a regulator of cell proliferation, angiogenesis, cell remodelling and other cellular processes. CTGF is necessary for normal follicle growth and luteinisation in mammals. The avi...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2012.06.018
更新日期：2012-09-01 00:00:00
abstract：:Changes in plasma levels of steroid hormones in pre-spawning chum salmon (Oncorhynchus keta) were examined for 6 years in association with sexual maturation. Fish were sampled along their homing pathway from the coastal sea to the spawning ground from 1995 to 2000. Plasma levels of testosterone (T), 11-ketotestosteron...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/s0016-6480(03)00171-0
更新日期：2003-09-01 00:00:00
abstract：:Using frog ovarian follicles in vitro we assessed the role of the theca/epithelium (THEP) layer and granulosa cells in the production of steroids. The THEP layer and granulosa cell-enclosed oocytes (GCEOs) were separated from ovarian follicles of the frog, Rana nigromaculata, by microdissection. Intact follicles (IFs)...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1994.1077
更新日期：1994-05-01 00:00:00
abstract：:Neuropeptides are ancient endocrine components which have evolved to regulate many aspects of biology across the animal kingdom including behaviour, development and metabolism. To supplement current knowledge, we have utilized a transcriptome series describing larval development in the ornate spiny lobster, Panulirus ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2020.113496
更新日期：2020-08-01 00:00:00
abstract：:Orexin A (OXA), a hypothalamic neuropeptide, and its receptor (OX1R) are primarily expressed in lateral hypothalamus and are involved in the control of various biological functions. Expressions of OXA and OX1R have also been reported in peripheral organs like gastrointestinal and genital tracts. In the present study, ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2015.11.005
更新日期：2016-12-01 00:00:00
abstract：:Chicken growth hormone secretagogue receptor (GHSR) is a receptor for ghrelin (GHRL), a peptide hormone produced by chicken proventriculus, which stimulates growth hormone (GH) release and food intake. The purpose of this study was to search for single nucleotide polymorphisms (SNPs) in exon 2 of GHSR gene and to anal...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2016.08.016
更新日期：2016-10-01 00:00:00
abstract：:The insulin receptors of frog brain and liver show features typical of other insulin receptors with regard to affinity and specificity of binding to insulins and proinsulin, solubility in Triton X-100, binding to and elution from wheat germ agglutinin, and insulin-sensitive tyrosine kinase activity. Likewise, the brai...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(87)90026-8
更新日期：1987-11-01 00:00:00
abstract：:The objective of this study was to examine if RU486, a corticosteroid antagonist, modified hepatocyte metabolism and blocked the metabolic effects of exogenously administered cortisol in rainbow trout (Oncorhynchus mykiss). The fish were given a single intraperitoneal implant of either coconut oil alone or coconut oil...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1994.1160
更新日期：1994-10-01 00:00:00
abstract：:Seasonal changes in testicular structure and function in three species of Korean frogs (Rana nigromaculata, R. rugosa, and R. dybowskii) having different breeding seasons and habitats were examined throughout the year. R. nigromaculata live in rice fields and breed in early May. Their gonadosomatic index (GSI) and tes...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1998.7118
更新日期：1998-09-01 00:00:00
abstract：:Gonadotropin-releasing hormone (GnRH) plays a central role in vertebrate reproduction. The evolutionary origin of this neuropeptide and its receptor is not obvious, but the advent of genomics makes it possible to examine the roots of GnRH and delve deeper into its ancestral relationships. New peptide sequences identif...
journal_title：General and comparative endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.ygcen.2010.12.014
更新日期：2011-03-01 00:00:00
abstract：:The neuropeptide gonadotropin-releasing hormone (GnRH) plays an important role in the control of reproductive functions. Vertebrates possess multiple GnRH isoforms that are classified into three main groups, namely GnRH1, GnRH2 and GnRH3. In the present study, we show that the chromosomal organization of the three GnR...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2010.11.017
更新日期：2011-02-01 00:00:00
abstract：:GPR-30, now named as GPER (G protein-coupled estrogen receptor) was first identified as an orphan receptor and subsequently shown to be required for estrogen-mediated signaling in certain cancer cells. Later studies demonstrated that GPER has the characteristics of a high affinity estrogen membrane receptor on Atlanti...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2014.11.011
更新日期：2015-01-15 00:00:00
abstract：:Differential transfer of maternal testosterone (T) into egg yolk provides a means of adjusting an offspring's phenotype to ambient environmental conditions. While the environmental and genetic driven variability in yolk T levels is widely described, the underlying mechanisms are poorly understood. Here, we investigate...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2017.05.004
更新日期：2018-01-15 00:00:00
abstract：:Secretin (Sct), traditionally a gastrointestinal hormone backed by a century long research, is now beginning to be recognized also as a neuroactive peptide. Substantiation by recent evidence on the functional role of Sct in various regions of the brain, especially on its potential neurosecretion from the posterior pit...
journal_title：General and comparative endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.ygcen.2012.11.017
更新日期：2013-01-15 00:00:00
abstract：:The reproductive biology of the Indian major carp Cirrhinus mrigala is tightly synchronized with the seasonal changes in the environment. While the ovaries show growth from February through June, the fish spawn in July-August to coincide with the monsoon; thereafter the fish pass into the postspawning and resting phas...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/s0016-6480(03)00190-4
更新日期：2003-10-01 00:00:00
abstract：:In fish there are few data on the gonadotropin-releasing hormone (Gn-RH) neurosecretory activity, which could explain long- and short-term variations of the gonadotropin secretion. There is no biological species specificity between mammal and fish Gn-RH; although there is a structural difference, they are, on the cont...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(86)90255-8
更新日期：1986-01-01 00:00:00
abstract：:Adrenomedullin (AM) is a multifunctional evolutionarily highly conserved peptide. Although its genomic and amino acid (aa) sequences are known in several mammalian species and in fish, the structure of the AM gene remains unknown in intermediate phyla, including birds. Here, we report the structure and aa sequence of ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2005.02.014
更新日期：2005-08-01 00:00:00
abstract：:To understand how the social and physical environment influences behaviour, reproduction and survival, studies of underlying hormonal processes are crucial; in particular, interactions between stress and reproductive responses may have critical influences on breeding schedules. Several authors have examined the timing...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2011.05.006
更新日期：2011-08-01 00:00:00
