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Characterization of adrenomedullin in birds.

Abstract：

:Adrenomedullin (AM) is a multifunctional evolutionarily highly conserved peptide. Although its genomic and amino acid (aa) sequences are known in several mammalian species and in fish, the structure of the AM gene remains unknown in intermediate phyla, including birds. Here, we report the structure and aa sequence of the chicken (c) AM ortholog. The cAM gene is located at the short arm of chromosome 5, which shows high synteny with the short arm of human (h) chromosome 11, where hAM is located. Key sequences in the third intron have been conserved which allow for an alternative splicing mechanism, similar to the one found in mammals. The preprohormone contains two peptides with high homology to human proadrenomedullin N-terminal 20 peptide (PAMP) and hAM. We found through real-time PCR and immunocytochemistry cAM mRNA and peptide expression in a variety of chicken tissues, which parallel patterns observed for mammals, with the exception that cAM levels are almost non-detectable in brain. Similarly to mammals, cAM expression is upregulated under hypoxic conditions and following dexamethasone treatment. These data demonstrate a high degree of homology between the cAM gene and its mammalian ortholog and evolutionary conservation of the regulatory mechanisms controlling its expression.

journal_name

Gen Comp Endocrinol

journal_title

General and comparative endocrinology

authors

Zudaire E,Cuesta N,Martínez A,Cuttitta F

doi

10.1016/j.ygcen.2005.02.014

keywords:

subject

Has Abstract

pub_date

2005-08-01 00:00:00

pages

10-20

issue

1

eissn

0016-6480

issn

1095-6840

pii

S0016-6480(05)00059-6

journal_volume

143

pub_type

杂志文章

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