Abstract：
:Glucagon plays a pivotal role in the regulation of metabolism. A glucagon receptor has been previously characterized in the frog, Rana tigrina rugulosa, and the frog and human glucagon receptors have been shown to possess similar binding affinities toward human glucagon. To study the structural evolution of glucagon peptide and its receptor in vertebrates, in the current study, a proglucagon cDNA from the same frog species was cloned. Interestingly, in contrast to the mammalian proglucagons that contain only one GLP-1 peptide, the frog proglucagon cDNA encodes two GLP-1 peptides (GLP-1A and GLP-1B) in addition to a glucagon peptide and a glucagon-like peptide 2 (GLP-2). By reverse transcriptase-PCR (RT-PCR) analysis, the proglucagon gene expression was widely detected in the brain, colon, small intestine, liver, lung, and pancreas, suggesting that the proglucagon-derived peptides have diverse functions in frogs. Moreover, tissue-specific alternative mRNA splicing was observed in the brain, colon, and pancreas. In these tissues, proglucagon transcripts with a 135 bp in frame deletion encoding GLP-1A were found. This splicing event in R. tigrina rugulosa is novel because it deletes a GLP-1 encoding sequence instead of the GLP-2 observed in other vertebrates. These findings should enhance understanding of the proglucagon evolution, structure, and expression in vertebrates.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Yeung CM,Chow BKdoi
10.1006/gcen.2001.7697keywords:
subject
Has Abstractpub_date
2001-11-01 00:00:00pages
144-51issue
2eissn
0016-6480issn
1095-6840pii
S0016-6480(01)97697-Xjournal_volume
124pub_type
杂志文章abstract：:PCB 77 is a dioxin-like PCB that has been shown to reduce circulating thyroid hormone (TH) levels. This may be an important factor contributing to its neurotoxicity, since THs are essential for normal brain development. In this study, we investigated the changes in TH activating and inactivating iodothyronine deiodina...
journal_title：General and comparative endocrinology
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journal_title：General and comparative endocrinology
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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journal_title：General and comparative endocrinology
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journal_title：General and comparative endocrinology
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pub_type： 杂志文章
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更新日期：2020-04-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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更新日期：2013-09-15 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2004.05.010
更新日期：2004-09-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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更新日期：2021-01-15 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2009.06.003
更新日期：2010-01-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(85)90406-x
更新日期：1985-09-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2008.04.010
更新日期：2008-06-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2015.12.002
更新日期：2017-02-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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更新日期：1992-01-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章,评审
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2014.07.028
更新日期：2014-09-15 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(86)90025-0
更新日期：1986-10-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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更新日期：1988-07-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2006.05.012
更新日期：2006-11-01 00:00:00
abstract：:Seasonally-breeding amphibians have served as excellent animal models to investigate the biosynthesis and biological actions of neurosteroids. Previous studies have demonstrated that the brain of amphibians possesses key steroidogenic enzymes and produces pregnenolone, a precursor of steroid hormones, and other variou...
journal_title：General and comparative endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.ygcen.2011.11.020
更新日期：2012-05-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.2000.7519
更新日期：2001-07-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2018.08.002
更新日期：2019-07-01 00:00:00
abstract：:For grass carp (Ctenopharyngodon idella) raised in the Ivory Coast (with water temperatures of 26-31 degrees C), induced spawning is obligatory for fry production. However, ovulation rates following hormonal treatment are often low. We hypothesized that high temperatures are an inhibiting factor for the reproductive a...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2003.12.009
更新日期：2004-04-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2016.03.001
更新日期：2017-01-15 00:00:00
abstract：:This study compares follicular function and ovulatory efficiency in 20 sows with obesity/leptin resistance genotype (Iberian pig) and 20 females of lean commercial crosses (Large White × Landrace; LW×L). Estrous cycle was synchronized with progestagens; ovulation was induced with eCG and hCG, in half of the females of...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2010.10.006
更新日期：2011-01-01 00:00:00
