In-vitro and in-vivo biological activity of recombinant yellowtail kingfish (Seriola lalandi) follicle stimulating hormone.

Abstract:

:Biologically active recombinant yellowtail kingfish follicle stimulating hormone (rytkFsh) was produced in yeast Pichia pastoris and its biological activity was demonstrated by both in-vitro and in-vivo bioassays. Incubation of ovarian and testicular fragments with the recombinant hormone stimulated E2 and 11-KT secretion, respectively. In-vivo trial in immature female YTK resulted in a significant increase of plasma E2 levels and development of oocytes. In males at the early stages of puberty, advancement of spermatogenesis was observed, however plasma 11-KT levels were reduced when administered with rytkFsh.

journal_name

Gen Comp Endocrinol

authors

Sanchís-Benlloch PJ,Nocillado J,Ladisa C,Aizen J,Miller A,Shpilman M,Levavi-Sivan B,Ventura T,Elizur A

doi

10.1016/j.ygcen.2016.03.001

subject

Has Abstract

pub_date

2017-01-15 00:00:00

pages

41-49

eissn

0016-6480

issn

1095-6840

pii

S0016-6480(16)30045-4

journal_volume

241

pub_type

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