Abstract：
:In teleosts, sex is plastic and is influenced by environmental factors. Elevated temperatures have masculinizing effects on the phenotypic sex of certain sensitive species. In this study, we reared genetic XX Japanese flounder at a high temperature (27.5 ± 0.5 °C) and obtained a population of sex-reversal XX males (male ratio, 95.24%). We comparatively analyzed the dynamic characteristics of germ cells and gsdf (gonadal soma-derived factor) expression during sexual differentiation for the experimental (27.5 ± 0.5 °C) and control (18 °C ± 0.5 °C) groups. The results revealed that the germ cell proliferation inhibited and gsdf expression up-regulated in the experimental group, and the gsdf mRNA and proteins expressed in somatic cells that had direct contact with germline stem cells (with Nanos 2 protein expression) including spermatogonia and oogonia by ISH (in situ hybridization) and IHC (immunohistochemistry). In addition, we also overexpressed the gsdf in XX flounders, and the germ cell number of XX flounders bearing gsdf gene significantly decreased and sometimes disappeared completely, which was consistent with the results from high-temperature induction. Therefore, based on all the results, we speculated that the high expression of gsdf might inhibit germ cell proliferation during sex differentiation, and eventually cause sex reversal in the high-temperature induced masculinization of XX Japanese flounder.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Yang Y,Liu Q,Xiao Y,Xu S,Wang X,Yang J,Song Z,You F,Li Jdoi
10.1016/j.ygcen.2018.12.012subject
Has Abstractpub_date
2019-04-01 00:00:00pages
17-25eissn
0016-6480issn
1095-6840pii
S0016-6480(18)30485-4journal_volume
274pub_type
杂志文章abstract：:Spexin (SPX), a novel peptide coevolved with the galanin/kisspeptin family, was first identified by bioinformatics prior to its protein purification/functional studies. Its mature peptide is highly conserved among different vertebrate classes. Based on the studies in mammals and fish models, SPX was found to be widely...
journal_title：General and comparative endocrinology
pub_type： 杂志文章,评审
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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journal_title：General and comparative endocrinology
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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journal_title：General and comparative endocrinology
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journal_title：General and comparative endocrinology
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1994.1012
更新日期：1994-01-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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更新日期：1983-12-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(83)90065-5
更新日期：1983-09-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2014.08.014
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2005.11.010
更新日期：2006-05-15 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2011.02.027
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(88)90024-x
更新日期：1988-03-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2010.11.026
更新日期：2011-03-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2018.12.003
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1998.7135
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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更新日期：2019-04-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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更新日期：1989-02-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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更新日期：2019-05-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2014.04.017
更新日期：2014-09-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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更新日期：1987-10-01 00:00:00
abstract：:The ability of hormones to bind to their functional receptors on turtle (Pseudemys scripta) endocrine target tissues in the cold was tested by treating tissues with secretagogues at low temperatures (5-15 degrees) and then following subsequent target stimulation in the absence of secretagogue at a warm temperature (28...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
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更新日期：1990-12-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2008.02.007
更新日期：2008-05-15 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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更新日期：1984-03-01 00:00:00
abstract：:The neotropical genus Mabuya are obligate placentotrophic viviparous lizards, which have a short vitellogenesis that produces microlecithal oocytes and a prolonged time of gestation (9 to 10 months). The hormonal control of female reproductive activity during follicular growth and pregnancy has not been studied, altho...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
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更新日期：2020-09-01 00:00:00
abstract：:Flatfishes with more body height after metamorphosis should be better adapted to a benthic lifestyle. In this study, we quantified the changes in body height during metamorphosis in two flatfish species, Paralichthys olivaceus and Platichthys stellatus. The specific pattern of cell proliferation along the dorsal and v...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2016.06.028
更新日期：2016-09-15 00:00:00
