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Prolactin receptors in tilapia (Sarotherodon mossambicus) tissues: binding studies using 125I-labeled ovine prolactin.

Abstract：

:The binding of 125I-labeled ovine prolactin (oPRL) to membrane preparations of tissue from freshwater-adapted tilapia (Sarotherodon mossambicus) was examined. Liver, ovary, and testis showed a relatively high specific binding (5-10%). A lower specific binding occurred consistently in intestine and gill tissue, and inconsistently in urinary bladder and kidney preparations. Desaturation experiments with MgCl2 indicated that a majority of the PRL receptors were already occupied by endogenous PRL. Scatchard analysis of liver binding gave a dissociation constant of 0.6 X 10(-9) M and a capacity of 207 fmol/mg protein.

journal_name

Gen Comp Endocrinol

journal_title

General and comparative endocrinology

authors

Edery M,Young G,Bern HA,Steiny S

doi

10.1016/0016-6480(84)90056-x

subject

Has Abstract

pub_date

1984-10-01 00:00:00

pages

19-23

issue

1

eissn

0016-6480

issn

1095-6840

pii

0016-6480(84)90056-X

journal_volume

56

pub_type

杂志文章

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