Gonadotropin-releasing hormone from thai catfish: chromatographic and physiological studies.

Abstract:

:Two forms of immunoreactive gonadotropin-releasing hormone (GnRH) were extracted from brain-pituitary tissues of Thai catfish, Clarias macrocephalus and C. batrachus. The peptides were detected using high performance liquid chromatography (HPLC) and radioimmunoassay (RIA). In both the HPLC systems, catfish GnRH-I eluted earlier than catfish GnRH-II and also eluted before the synthetic standards of mammalian, lamprey, chicken I, chicken II, and salmon GnRH. Hence, catfish GnRH-I appears to be the most hydrophilic GnRH family member because of this early elution from the HPLC. Catfish GnRH-II eluted in a position similar to that of chicken GnRH-II. This study suggests that catfish GnRH-I is a novel form of GnRH, whereas catfish GnRH-II is the same as chicken GnRH-II. Indirect evidence suggests that the catfish molecule is 10 amino acids in length and has an amide at the C-terminus. Moreover, the novel catfish GnRH appears to be different within the domain of amino acids 5 to 10 compared with mammalian GnRH because it is not recognized by antiserum B-6. An injection of native chicken GnRH-II was more effective than salmon or mammalian GnRH for induced ovulation in C. macrocephalus.

journal_name

Gen Comp Endocrinol

authors

Ngamvongchon S,Sherwood NM,Warby CM,Rivier JE

doi

10.1016/0016-6480(92)90031-e

keywords:

subject

Has Abstract

pub_date

1992-08-01 00:00:00

pages

266-74

issue

2

eissn

0016-6480

issn

1095-6840

pii

0016-6480(92)90031-E

journal_volume

87

pub_type

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