Abstract:
:The public deposition of large transcriptome shotgun assembly (TSA) datasets for the Araneae (true spiders) provides a resource for determining the structures of the native neuropeptides present in members of this chelicerate order. Here, the Araneae TSA data were mined for putative peptide-encoding transcripts using the recently deduced neuropeptide precursors from the Western black widow Latrodectus hesperus as query templates. Neuropeptide-encoding transcripts from five spiders, Latrodectus tredecimguttatus, Stegodyphus mimosarum, Stegodyphus lineatus, Stegodyphus tentoriicola and Acanthoscurria geniculata, were identified, including ones encoding members of the allatostatin A, allatostatin B, allatostatin C, allatotropin, CAPA/periviscerokinin/pyrokinin, crustacean cardioactive peptide, crustacean hyperglycemic hormone/ion transport peptide, diuretic hormone 31, diuretic hormone 44, eclosion hormone, FMRFamide-like peptide (FLP), GSEFLamide, insulin-like peptide, orcokinin, proctolin, short neuropeptide F, SIFamide, sulfakinin and tachykinin-related peptide (TRP) families. A total of 156 distinct peptides were predicted from the precursor proteins deduced from the S. mimosarum transcripts, with 65, 26, 21 and 12 peptides predicted from those deduced from the A. geniculata, L. tredecimguttatus, S. lineatus and S. tentoriicola sequences, respectively. Among the peptides identified were variant isoforms of FLP, orcokinin and TRP, peptides whose structures are similar to ones previously identified from L. hesperus. The prediction of these atypical peptides from multiple spiders suggests that they may be broadly conserved within the Araneae rather than being species-specific variants. Taken collectively, the data described here greatly expand the number of known Araneae neuropeptides, providing a foundation for future functional studies of peptidergic signaling in this important Chelicerate order.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Christie AE,Chi Mdoi
10.1016/j.ygcen.2015.02.003subject
Has Abstractpub_date
2015-03-01 00:00:00pages
90-109eissn
0016-6480issn
1095-6840pii
S0016-6480(15)00040-4journal_volume
213pub_type
杂志文章abstract::The gross morphology, histology, and ultrastructure of the thyroid gland of the koala, Phascolarctos cinereus, is described. Generally, the glands were found to contain large-diameter follicles in association with an epithelium of low height. Morphometric analysis demonstrated a high relative thyroid weight (0.3 +/- 0...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1998.7076
更新日期:1998-06-01 00:00:00
abstract::The relative affinities and maximum capacities of the classes of L-thyroxine (T4)- and 3,5,3'-triiodo-L-thyronine (T3)-binding sites in plasma of three species of salmonid teleost fish were determined by saturation analysis on miniature Sephadex G-25 columns at 20-21 degrees and kinetic data were analyzed by the multi...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(87)90176-6
更新日期:1987-02-01 00:00:00
abstract::Dopamine (DA) was infused into the third ventricle of anesthetized laying turkey hens at various concentrations to determine its effect on both basal prolactin (PRL) levels and ongoing electrically induced PRL secretion. The infusion of DA at rates of 1.0 or 10.0 nmol/min resulted in dose dependent increases in plasma...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1995.1049
更新日期:1995-04-01 00:00:00
abstract::The distribution and properties of nonapeptide binding sites in the kidney of the anuran Xenopus laevis were investigated using quantitative in vitro autoradiography. The binding studies were performed with [3H]arginine vasopressin (AVP) as ligand because [125I]arginine vasotocin (AVT) lacks biological activity. Speci...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(92)90173-h
更新日期:1992-01-01 00:00:00
abstract::The skin of Xenopus laevis, because of its high concentrations of amidated peptides, is an excellent model for the study of neuropeptide biosynthesis. In this paper we determined levels of the mRNAs which encode cerulin and characterized the enzymatic activity which likely produces the carboxyl terminal alpha-amide mo...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(87)90206-1
更新日期:1987-07-01 00:00:00
abstract::The effects of prolonged nutrient restriction (fasting) and subsequent restoration (re-feeding) on the growth hormone (GH)/insulin-like growth factor (IGF) axis were investigated in the tilapia (Oreochromis mossambicus). Mean weight and specific growth rate declined within 1 week in fasted fish, and remained lower tha...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2009.11.012
更新日期:2010-05-01 00:00:00
abstract::This paper describes changes in the content of free steroid hormones e.g. testosterone (T), estradiol-17β (E2), estrone (E1) and estriol (E3) of Mytilus trossulus from the southern Baltic Sea as a function of season, stage of gametogenesis, sex, tissue (gonadal and somatic) and depth. The highest levels of T, E2, E1 a...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2017.12.006
更新日期:2018-04-01 00:00:00
abstract::Secretin (Sct), traditionally a gastrointestinal hormone backed by a century long research, is now beginning to be recognized also as a neuroactive peptide. Substantiation by recent evidence on the functional role of Sct in various regions of the brain, especially on its potential neurosecretion from the posterior pit...
journal_title:General and comparative endocrinology
pub_type: 杂志文章,评审
doi:10.1016/j.ygcen.2012.11.017
更新日期:2013-01-15 00:00:00
abstract::Several lines of evidence suggest reproduction in the ranid frogs is potently regulated by the gonadal steroids, in particular 5alpha-dihydrotestosterone (DHT) and 17beta-estradiol (E(2)), and a non-gonadal steroid, the stress hormone corticosterone (Cort). Little is known about how these steroid hormones act upon the...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2003.07.001
更新日期:2003-12-01 00:00:00
abstract::Using antibodies to the amino-terminal region of human parathyroid hormone-related protein (PTHrP) we have demonstrated PTHrP immunoreactivity in pituitaries and plasma of the sea bream (Sparus aurata). Pituitary cells at two distinct locations contained immunodetectable PTHrP; an anterior group in the rostral pars di...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1993.1156
更新日期:1993-11-01 00:00:00
abstract::The insulin-like growth factor-1 (IGF-1) plays an important role in the regulation of reproductive function. In the present study, we examined the effects of caloric restriction (CR) on the reproductive lifespan in rats and investigated the potential role of IGF-1. After 10 weeks of treatment, we determined the distri...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2011.09.005
更新日期:2011-11-01 00:00:00
abstract::Steelhead and hybrid trout (rainbow trout male, steelhead female) presmolts and smolts, Oncorhynchus mykiss, exposed to 30/1000 seawater (SW) for 24 hr manifested progressively lower plasma sodium values from December to May. The increase in plasma sodium after 24 hr in SW diminished significantly after March, with th...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1994.1012
更新日期:1994-01-01 00:00:00
abstract::Fasting animals must ration stored reserves appropriately for metabolic demands. Animals that experience fasting concomitant with other metabolically demanding activities are presented with conflicting demands of energy conservation and expenditure. Our objective was to understand how fasting northern elephant seals r...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2015.09.024
更新日期:2016-01-01 00:00:00
abstract::The effect of a gonadotropin-releasing hormone analog (GnRHA, HOE766) was studied in hypophysectomized elasmobranch fish Torpedo marmorata and T. ocellata. In addition, estradiol (E2) effects were studied in intact and hypophysectomized (HPX) animals. Plasma androgen concentrations were measured 2 or 6 hr after GnRHA ...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(89)90169-x
更新日期:1989-09-01 00:00:00
abstract::In teleosts, sex is plastic and is influenced by environmental factors. Elevated temperatures have masculinizing effects on the phenotypic sex of certain sensitive species. In this study, we reared genetic XX Japanese flounder at a high temperature (27.5 ± 0.5 °C) and obtained a population of sex-reversal XX males (ma...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2018.12.012
更新日期:2019-04-01 00:00:00
abstract::The purpose of the present study was to determine if central administration of somatostatin influences feeding behavior in layer chicks. Five- to 7-day-old chicks that received intracerebroventricular (ICV) injections of 0.5 or 2 nmol somatostatin increased their food intake at 30 and 60 min after the injection, sugge...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2009.01.022
更新日期:2009-05-01 00:00:00
abstract::The distribution of melatonin was studied at midday and midnight in pigeon plasma, pineal, hypothalamus, eyes, Harderian glands, and duodenum. Tissue samples were homogenized, extracted with chloroform, evaporated, and measured by our melatonin radioimmunoassay (RIA). All the extracts gave dilution curves parallel to ...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(85)90136-4
更新日期:1985-04-01 00:00:00
abstract::Neurotransmitters such as serotonin (5-hydroxytryptamine; 5-HT) in the central nervous system regulate diverse physiological functions, including reproduction, feeding, learning, and memory, in diverse animal phyla. 5-HT and the 5-HT1 subtype receptor play important roles in sexual maturation and in the initiation of ...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2019.03.001
更新日期:2019-05-15 00:00:00
abstract::The cell types within the endocrine pancreatic tissue and anterior intestine of larvae and juveniles of representatives of the two southern-hemisphere families (Mordaciidae and Geotriidae) were compared, using immunohistochemistry and antisera against insulin (lamprey, bovine), two somatostatins (SST-14, -34), two PP-...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1993.1152
更新日期:1993-11-01 00:00:00
abstract::Gonadotropin-releasing hormone (GnRH) and its receptor are essential for reproduction in vertebrates. Although there are three major types of GnRH peptides and two major types of receptors in vertebrates, the pattern of distribution is unusual. Evidence is presented from genome mining that type I GnRHRs are not restri...
journal_title:General and comparative endocrinology
pub_type: 杂志文章,评审
doi:10.1016/j.ygcen.2014.07.025
更新日期:2014-12-01 00:00:00
abstract::Previous investigations have shown that the release of PRL and GH from the tilapia pituitary is directly sensitive to osmotic pressure and a variety of endocrine and neuroendocrine factors. The present studies were aimed at determining whether the spontaneous release of PRL and GH, in vitro, is: (1) sensitive to the n...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(92)90059-s
更新日期:1992-06-01 00:00:00
abstract::We investigated the effect of several potential carbohydrate secretagogues, amino acids, a ketoacid, and potassium chloride on insulin, glucagon, and somatostatin release from the in vitro perfused Brockmann body of channel catfish (Ictalurus punctatus). Mannose (15 mM) stimulated the release of insulin and somatostat...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(87)90120-1
更新日期:1987-03-01 00:00:00
abstract::Application of alpha-MSH immunohistochemistry to the brain of elasmobranchs (Scyliorhinus torazame and Etmopterus brachyurus) demonstrated a marked species difference concerning the distribution of the alpha-MSH-like molecule in the brain. In S. torazame, alpha-MSH-like immunoreactive cells were present in the hypotha...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.2001.7628
更新日期:2001-06-01 00:00:00
abstract::Prolactin-like protein (PRL-L; LOC417800) is a homolog of PRL in non-mammalian vertebrates and can act as a functional ligand of PRL receptor (PRLR). Despite its widespread expression in extrapituitary tissues, mechanisms of regulation of PRL-L in the chicken ovary remain unknown. In this study, we first examined PRL-...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2016.10.013
更新日期:2017-01-01 00:00:00
abstract::In addition to the previously recognised thyroid hormone-binding proteins, three plasma components possibly involved in T3 and T4 transport have been identified in chicken plasma using gel filtration at pH 7.4. In mature female, though not male and immature female birds, a significant amount of T3 (11%) is bound to ve...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(85)90276-x
更新日期:1985-02-01 00:00:00
abstract::Goldfish GH-releasing hormone (gGHRH) has been recently identified and shown to stimulate GH release in goldfish. In goldfish, neuroendocrine regulation of GH release is multifactorial and known stimulators include goldfish ghrelin (gGRLN19) and salmon gonadotropin-releasing hormone (sGnRH), factors that also enhance ...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2013.02.037
更新日期:2013-06-01 00:00:00
abstract::Circannual and circadian variations in plasma levels of steroids were estimated by radioimmunoassay in the female and male catfish, Heteropneustes fossilis, over two consecutive annual reproductive cycles. In the female catfish, testosterone (T), estradiol-17 beta (E2), and estrone (E1) were detectable in the plasma o...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(83)90221-6
更新日期:1983-05-01 00:00:00
abstract::The first studies that identified leptin and its receptor (LepR) in mammals were based on mutant animals that displayed dramatic changes in body-weight and regulation of energy homeostasis. Subsequent studies have shown that a deficiency of leptin or LepR in homoeothermic mammals results in hyperphagia, obesity, infer...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract::In recent years, invertebrate peptides have been identified which share substantial homologies with vertebrate insulin and insulin-like growth factors (IGFs), indicating a high degree of conservation of insulin/IGF systems through animal evolution. In a previous study, we provided evidence for the presence of IGF-I-li...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1996.6824
更新日期:1997-03-01 00:00:00
abstract::The present study examined plasma concentrations of insulin-like growth factor (IGF)-I, IGF-II and IGF-binding proteins (IGFBPs) during posthatch growth and development in chickens. Three distinct proteins which bound 125I-IGF-I were observed irrespective of age or sex, these having apparent molecular weights of 22, 2...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1997.6898
更新日期:1997-07-01 00:00:00