Abstract：
:Prolactin (PRL) and two variants of growth hormone (GH), purified from pituitaries of striped bass (Morone saxatilis) and its hybrid with white bass (M. saxatilis x M. chrysops) by gel filtration chromatography under alkaline conditions followed by reversed-phase high pressure liquid chromatography, appear similar between species. Both the minor (GH I) and the major (GH II) forms of purified GH appeared as single bands (M(r) approximately 23,000) after sodium dodecyl sulfate-polyacrylamide gel electrophoresis, as did the purified PRL (M(r) approximately 24,000). The molecular weights of GH II and PRL determined by MALDI TOF mass spectroscopy were 21.2 and 21.3 kDa, respectively. In Western blotting experiments, an antiserum against tilapia (Oreochromis mossambicus) 24K PRL specifically recognized Morone PRL, while an antiserum against tilapia GH specifically recognized Morone GH I and II. Chemical identities of the putative PRL and GH I were further confirmed by N-terminal peptide sequencing, while internal sequence analysis was performed on GH II because it was blocked at its N-terminus. Over a stretch of 29 amino acids, Morone PRL was found to be 76% identical to tilapia 24K PRL, 72% identical to tilapia 20K PRL, 72% identical to chum salmon (Oncorhynchus keta) PRL I, and 69% identical to eel (Anguilla japonica) PRL I. Alignment of the hybrid striped bass GH sequences with those of several other advanced marine teleosts indicated 75-85% sequence identity for GH I (40 amino acids) and 95-98% identity for GH II (45 amino acids). Biological activity of striped bass GH II was confirmed using a heterologous in vitro assay of insulin-like growth factor I mRNA production by coho salmon (On. kisutch) hepatocytes. An in vivo bioassay, involving hypophysectomy of hybrid striped bass and treatment of the fish maintained in fresh water with homologous PRL, confirmed that the purified striped bass PRL was also bioactive.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Jackson LF,Swanson P,Duan C,Fruchtman S,Sullivan CVdoi
10.1006/gcen.1999.7399keywords:
subject
Has Abstractpub_date
2000-01-01 00:00:00pages
138-50issue
1eissn
0016-6480issn
1095-6840pii
S0016-6480(99)97399-9journal_volume
117pub_type
杂志文章abstract：:To characterize the involvement of the aromatase gene during the process of sex determination in the European eel (Anguilla anguilla), the expression of its gonadal form was determined during various developmental stages. The cloned cDNA from the European eel gonad (EeCYP19) contains an open reading frame of 1539 bp, ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2004.06.007
更新日期：2004-09-15 00:00:00
abstract：:We studied effects of in vitro conditions on the steady-state distribution and exchange of thyroid hormones (TH) between red blood cells (RBC) and plasma of rainbow trout. At steady state at 12 degrees C the RBC contained 5-11% of L-thyroxine (T4), 14-23% of 3,5,3'-triiodo-L-thyronine (T3), and 23-24% of 3,3',5'-triio...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1997.7028
更新日期：1998-02-01 00:00:00
abstract：:The courtship behavior of the male ring dove (Streptopelia risoria) combines aggressive displays (chasing, bowing) and nest-oriented displays (nest soliciting). Aggressive displays depend on circulating testosterone, whereas nest soliciting is estrogen-dependent and appears to depend on the aromatization of androgen i...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.2001.7608
更新日期：2001-04-01 00:00:00
abstract：:The effects of fasting and feeding on the brain expression of orexin (OX), tyrosine hydroxylase (TH), peptide Y (PY) and cholecystokinin (CCK) were examined in the blind cavefish Astyanax fasciatus mexicanus. A 10-days fasting period induced increases in both OX and TH brain mRNA expression but had no effect on PYY an...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2012.12.011
更新日期：2013-03-01 00:00:00
abstract：:The in vivo actions of human and fish insulin-like growth factor (IGF)-I have been compared to extend the understanding of the metabolism of IGFs in fish and to identify potential differences in their actions. The effects of acute administration of these proteins on the incorporation of glucose into muscle glycogen an...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1999.7417
更新日期：2000-03-01 00:00:00
abstract：:We gave an accurate immunolocalization of CHH (crustacean hyperglycemic hormone) and VIH (vitellogenesis-inhibiting hormone) in the brain and the sinus gland of the woodlouse Armadillidium vulgare. The two immune sera have been respectively raised against HPLC-purified CHH and against a small peptide derived from the ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/s0016-6480(02)00645-7
更新日期：2003-04-01 00:00:00
abstract：:The aim of the present study was to determine the relationship between seasonal variation in levels of insulin, cortisol, and corticosterone and body mass in a natural population of golden-mantled ground squirrels (Spermophilus saturatus) over the active season, from April to August. The body mass of females is at an ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1994.1189
更新日期：1994-12-01 00:00:00
abstract：:The aim of this study was to identify the major C21 steroids produced in vivo during artificially induced final oocyte maturation and spawning in female common dentex (Dentex dentex). During the spawning season, mature females were treated with a gonadotropin-releasing hormone agonist (GnRHa)-loaded delivery system, w...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.2000.7519
更新日期：2001-07-01 00:00:00
abstract：:Expression of the tilapia prolactin receptor (tiPRL-R) has been characterized in the intestine of Oreochromis niloticus and the levels of both tiPRL-R transcripts and tiPRL binding sites have been further analyzed in this organ, as well as in gill and kidney, during adaptation of tilapia to a hyperosmotic environment....
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.2001.7672
更新日期：2001-09-01 00:00:00
abstract：:The neuroendocrine system integrates genotype with external factors such as nutrition to regulate animal growth. To investigate the role of somatotropic axis in the interaction of genotype and nutrition, two series of experiments were conducted using broiler and layer chickens as a model. In the first experiment, both...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2003.11.009
更新日期：2004-03-01 00:00:00
abstract：:In crustaceans, vitellogenin (VTG, the precursor of major yolk protein) is synthesized in the ovary and/or hepatopancreas, and its synthesis is considered to be under the negative control of the vitellogenesis-inhibiting hormone (VIH), a neuropeptide secreted from the X-organ/sinus gland complex in the eyestalks. In t...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2006.03.005
更新日期：2006-09-01 00:00:00
abstract：:Insulin-like peptides (ILPs) regulate numerous functions in insects including growth, development, carbohydrate metabolism and female reproduction. This paper reports the immunohistochemical localization of ILPs in brain neurons of Rhodnius prolixus and their intimate associations with the brain circadian clock system...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2012.08.018
更新日期：2012-11-01 00:00:00
abstract：:A protein with insulin-like effects was isolated from the hepatopancreas of the lobster Panulirus argus following a classic method for mammalian insulin purification from the pancreas. After acid-alcoholic extraction and ethanol-ether precipitation followed by molecular filtration chromatography, a protein with an app...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/s0016-6480(03)00014-5
更新日期：2003-05-01 00:00:00
abstract：:Ecdysis, or the shedding of the old cuticle, depends on coordinated stereotyped behaviors, regulated by a number of neuropeptides. In the hornworm, Manduca sexta, two neuropeptides interact, namely ecdysis-triggering hormone (ETH) and eclosion hormone. We looked at the effects of ETH in vivo and in vitro, on the brain...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2009.08.008
更新日期：2010-03-01 00:00:00
abstract：:Cardiovascular responses to synthetic eel [Asn1, Val5, Gly9]-ANG-I, ANG-III (2-8), and ANG-I (1-7) were measured in conscious and resting freshwater North American eels. Indwelling Doppler flow probes were placed on the ventral and dorsal aortas, a pressure catheter in the lienomesenteric artery, and a peptide deliver...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1995.1025
更新日期：1995-02-01 00:00:00
abstract：:We have studied the effects of bovine parathyroid hormone (PTH) and salmon calcitonin (CT) on plasma calcium (Ca) and phosphate levels and on renal function in intact and parathyroidectomized (PTX) chickens, using a simple technique that separates urine from feces. In immature males and egg-laying females, the intrave...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(87)90248-6
更新日期：1987-06-01 00:00:00
abstract：:The titer of juvenile hormone (JH), which has wide ranging physiological effects in insects, is regulated in part by JH esterase (JHE). We show that overexpression in Drosophila melanogaster of the JHE binding protein, DmP29 results in a series of apparent anti-JH effects. We hypothesize that DmP29 functions in transp...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2008.01.006
更新日期：2008-03-01 00:00:00
abstract：:In teleost fish, the predominant brain form of cytochrome P450 aromatase (P450aromB) is a neural marker of estrogen effect, and an entry point for studying the role of hormonal and environmental estrogens on neurodevelopment and neuroplasticity. As part of a project using zebrafish to investigate these issues, we deve...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2005.12.010
更新日期：2006-06-01 00:00:00
abstract：:Three gonadotropin-releasing hormones (GnRHs) and three cognate receptors have been identified in vertebrates, with distinct distributions and functions. According to their sequences, the receptors can be grouped into distinct classes: types I, II, and III. One branch contains all type-I GnRH receptors (GnRH-R-I) from...
journal_title：General and comparative endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.ygcen.2005.01.019
更新日期：2005-05-15 00:00:00
abstract：:We analyzed the participation of sympathetic ovarian innervation in the prepubertal female guinea pig on regulation of compensatory ovarian hypertrophy (COH) and compensatory ovulation at puberty. The COH of the left ovary was significantly higher that of the right one (left ovary: 41.5+/-5.2 vs. 27.5+/-5.6%, p<0.05, ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/s0016-6480(02)00061-8
更新日期：2002-07-01 00:00:00
abstract：:Sexual behavior is one of the most profound events during the life cycle of animals that reproduce sexually. After completion of gonadal development that is mediated by various hormones, oviparous teleosts perform a suite of behaviors, often termed as spawning behavior. This is particularly important for teleosts that...
journal_title：General and comparative endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.ygcen.2009.04.011
更新日期：2010-02-01 00:00:00
abstract：:Intraperitoneal injection of rainbow trout (Salmo gairdneri) with 0.4 microgram/g of human growth hormone (hGH) increased plasma levels of 3,5,3'-triiodo-L-thyronine (T3) and Vmax of the hepatic microsomal 5'-monodeiodinase enzyme (5'D) that converts thyroxine (T4) to T3, with no effect on Km or plasma T4 levels. A do...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(90)90002-4
更新日期：1990-05-01 00:00:00
abstract：:In fish there are few data on the gonadotropin-releasing hormone (Gn-RH) neurosecretory activity, which could explain long- and short-term variations of the gonadotropin secretion. There is no biological species specificity between mammal and fish Gn-RH; although there is a structural difference, they are, on the cont...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(86)90255-8
更新日期：1986-01-01 00:00:00
abstract：:Wrasse species exhibit a definite daily rhythm in locomotor activity and bury themselves in the sand at the bottom of the ocean at night. It remains unclear how their behavior in locomotor activity is endogenously regulated. The aim of the present study was to clarify the involvement of melatonin and clock genes (Per1...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2012.01.007
更新日期：2012-05-15 00:00:00
abstract：:Cholecystokinin (CCK) and gastrin-releasing peptide (GRP) are gastrointestinal peptides thought to be important regulators of intake and digestion of food in vertebrates. In this study, pre- and postprandial plasma levels of CCK and GRP were measured in rainbow trout (Oncorhynchus mykiss) by the establishment of homol...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2006.02.016
更新日期：2006-09-01 00:00:00
abstract：:Full-length complementary deoxyribonucleic acid as well as genomic sequences encoding for two gastrointestinal appetite-related peptides, ghrelin and for gastrin-releasing peptide (GRP) were cloned from Atlantic cod (Gadus morhua) stomach using reverse transcription and rapid amplification of complementary deoxyribonu...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2008.12.004
更新日期：2009-02-01 00:00:00
abstract：:Lampreys diverged from the jawed vertebrate lineage approximately 500million years ago. Lampreys undergo sex differentiation much later than most other vertebrates, and ovarian differentiation occurs several years before testicular differentiation. The genetic basis of lamprey sex differentiation is of particular inte...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2014.08.019
更新日期：2014-11-01 00:00:00
abstract：:Allatostatin-like immunoreactivity was demonstrated in the central nervous system (CNS) neurons of the freshwater pulmonate snails Bulinus globosus (Planorbidae) and Stagnicola elodes (Lymnaeidae). Immunopositive neurons were localized using a monoclonal antibody highly specific to allatostatin I (APSGAQRLYGFGL-amide)...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1996.6871
更新日期：1997-05-01 00:00:00
abstract：:Fecal glucocorticoid metabolite analysis is a useful tool for monitoring adrenocortical activity in captive and free-ranging wildlife. Glucocorticoid metabolites may not be evenly distributed within fecal samples and this variability could affect the interpretation of glucocorticoid metabolite measurements. Furthermor...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/s0016-6480(03)00061-3
更新日期：2003-06-01 00:00:00
abstract：:Estrogen signalling is critical for ovarian differentiation in reptiles with temperature-dependent sex determination (TSD). To elucidate the involvement of estrogen in this process, adrenal-kidney-gonadal (AKG) expression of estrogen receptor (ERα) was studied at female-producing temperature (FPT) in the developing em...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2014.08.003
更新日期：2015-09-01 00:00:00
