Locking mixed-lineage kinase domain-like protein in its auto-inhibited state prevents necroptosis.

abstract：:We have determined the nucleotide sequences of the Klebsiella pneumoniae nifL (regulation of N2 fixation genes) and the Escherichia coli glnA (glutamine synthetase) promoters. We compared these sequences with the published sequences of three other promoters that, like the nifL and glnA promoters, are activated by the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ow DW,Sundaresan V,Rothstein DM,Brown SE,Ausubel FM

更新日期：1983-05-01 00:00:00

abstract：:Neural crest stem cells can be isolated from differentiated cultures of human pluripotent stem cells, but the process is inefficient and requires cell sorting to obtain a highly enriched population. No specific method for directed differentiation of human pluripotent cells toward neural crest stem cells has yet been r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Menendez L,Yatskievych TA,Antin PB,Dalton S

更新日期：2011-11-29 00:00:00

abstract：:Efforts are underway to construct several recoded genomes anticipated to exhibit multivirus resistance, enhanced nonstandard amino acid (nsAA) incorporation, and capability for synthetic biocontainment. Although our laboratory pioneered the first genomically recoded organism (Escherichia coli strain C321.∆A), its fitn...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wannier TM,Kunjapur AM,Rice DP,McDonald MJ,Desai MM,Church GM

更新日期：2018-03-20 00:00:00

abstract：:We determined the complete nucleotide sequence of an infectious proviral molecular clone (FIV-14) of the feline immunodeficiency virus (FIV). FIV-14 has a genome organization similar in complexity to other lentiviruses. In addition to three large open reading frames representing the gag, pol, and env genes, at least f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Olmsted RA,Hirsch VM,Purcell RH,Johnson PR

更新日期：1989-10-01 00:00:00

abstract：:Combinatorial antibody libraries have the potential to display the entire immunological record of an individual, allowing one to detect and recover any antibody ever made, irrespective of whether it is currently being produced. We have termed this the "fossil record" of an individual's antibody response. To determine ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Felding-Habermann B,Lerner RA,Lillo A,Zhuang S,Weber MR,Arrues S,Gao C,Mao S,Saven A,Janda KD

更新日期：2004-12-07 00:00:00

abstract：:ATP is copackaged and coreleased with adrenergic, serotonergic, and cholinergic neurotransmitters, suggesting a possible interaction between the signaling pathways for ATP and these coreleased neurotransmitters. Muscarinic m2 and m4, alpha 2-adrenergic, and D2-dopaminergic neurotransmitter receptors, which have in com...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Felder CC,Williams HL,Axelrod J

更新日期：1991-08-01 00:00:00

abstract：:The single-pass transmembrane domains (TMDs) of the syndecan family of cell surface adhesion molecules have been implicated in functional protein-protein interactions. Although each paralog contains a conserved GxxxG dimerization motif, we show here that the syndecan-1 TMD dimerizes weakly, the syndecan-3 and syndecan...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dews IC,Mackenzie KR

更新日期：2007-12-26 00:00:00

abstract：:Microcalcifications geographically target the location of abnormalities within the breast and are of critical importance in breast cancer diagnosis. However, despite stereotactic guidance, core needle biopsy fails to retrieve microcalcifications in up to 15% of patients. Here, we introduce an approach based on diffuse...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Soares JS,Barman I,Dingari NC,Volynskaya Z,Liu W,Klein N,Plecha D,Dasari RR,Fitzmaurice M

更新日期：2013-01-08 00:00:00

abstract：:We previously developed a biological containment system using recombinant Salmonella Typhimurium strains that are attenuated yet capable of synthesizing protective antigens. The regulated delayed attenuation and programmed self-destructing features designed into these S. Typhimurium strains enable them to efficiently ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kong W,Brovold M,Koeneman BA,Clark-Curtiss J,Curtiss R 3rd

更新日期：2012-11-20 00:00:00

abstract：:The family of WD40-repeat (WDR) proteins is one of the largest in eukaryotes, but little is known about their function in brain development. Among 26 WDR genes assessed, we found 7 displaying a major impact in neuronal morphology when inactivated in mice. Remarkably, all seven genes showed corpus callosum defects, inc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kannan M,Bayam E,Wagner C,Rinaldi B,Kretz PF,Tilly P,Roos M,McGillewie L,Bär S,Minocha S,Chevalier C,Po C,Sanger Mouse Genetics Project.,Chelly J,Mandel JL,Borgatti R,Piton A,Kinnear C,Loos B,Adams DJ,Hérault Y,

更新日期：2017-10-31 00:00:00

abstract：:BSC-1 cells, epithelial cells of African green monkey kidney origin, show pronounced density-dependent regulation of growth in cell culture. Growth of the cells is rapid to a density of approximately 1.5 x 10(5) cells/per cm(2) in Dulbecco-modified Eagle's medium supplemented with 10% calf serum. Above this "saturatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Holley RW,Armour R,Baldwin JH

更新日期：1978-01-01 00:00:00

abstract：:The assembly of tiny magnetic particles in external magnetic fields is important for many applications ranging from data storage to medical technologies. The development of ever smaller magnetic structures is restricted by a size limit, where the particles are just barely magnetic. For such particles we report the dis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mehdizadeh Taheri S,Michaelis M,Friedrich T,Förster B,Drechsler M,Römer FM,Bösecke P,Narayanan T,Weber B,Rehberg I,Rosenfeldt S,Förster S

更新日期：2015-11-24 00:00:00

abstract：:High-performance anion-exchange (HPAE) chromatography under alkaline conditions (pH congruent to 13) has been found to efficiently separate neutral oligosaccharides (triose to undecaose) according to molecular size, sugar composition, and linkage of monosaccharide units. The method was able to resolve 1----3, 1----4, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hardy MR,Townsend RR

更新日期：1988-05-01 00:00:00

abstract：:The environments we humans encounter daily are sources of exposure to diverse microbial communities, some of potential concern to human health. In this study, we used culture-independent technology to investigate the microbial composition of biofilms inside showerheads as ecological assemblages in the human indoor env...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Feazel LM,Baumgartner LK,Peterson KL,Frank DN,Harris JK,Pace NR

更新日期：2009-09-22 00:00:00

abstract：:In Epstein-Barr virus (EBV)-positive Burkitt lymphoma (BL) the role of EBV in the translocation and deregulation of the MYC oncogene remains unknown. By utilizing an EBV-negative BL (BJAB) and several EBV-positive sublines derived from it by in vitro infection, it was possible to show that the presence of the virus wa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lacy J,Summers WP,Watson M,Glazer PM,Summers WC

更新日期：1987-08-01 00:00:00

abstract：:Pathogenic retroviruses have evolved multiple means for evading host restriction factors such as apolipoprotein B editing complex (APOBEC3) proteins. Here, we show that murine leukemia virus (MLV) has a unique means of counteracting APOBEC3 and other cytosolic sensors of viral nucleic acid. Using virus isolated from i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stavrou S,Nitta T,Kotla S,Ha D,Nagashima K,Rein AR,Fan H,Ross SR

更新日期：2013-05-28 00:00:00

abstract：:We have constructed bovine papilloma virus recombinants carrying a hybrid gene in which human growth hormone structural sequences are fused to the promoter and presumptive control region of the mouse metallothionein-I gene. Mouse cells transformed with the recombinants synthesize metallothionein-growth hormone hybrid ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pavlakis GN,Hamer DH

更新日期：1983-01-01 00:00:00

abstract：:A continuous fluorescence-based assay is described for measuring helicase-mediated unwinding of duplex DNA. The assay utilizes an oligonucleotide substrate containing the fluorescent adenine analog, 2-aminopurine, at regular intervals. 2-Aminopurine forms a Watson-Crick-type base pair with thymine and does not distort...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Raney KD,Sowers LC,Millar DP,Benkovic SJ

更新日期：1994-07-05 00:00:00

abstract：:Thiol-dependent redox regulation allows the rapid adaptation of chloroplast function to unpredictable changes in light intensity. Traditionally, it has been considered that chloroplast redox regulation relies on photosynthetically reduced ferredoxin (Fd), thioredoxins (Trxs), and an Fd-dependent Trx reductase (FTR), t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pérez-Ruiz JM,Naranjo B,Ojeda V,Guinea M,Cejudo FJ

更新日期：2017-11-07 00:00:00

abstract：:We propose and test a theoretical perspective in which a universal hallmark of successful literacy acquisition is the convergence of the speech and orthographic processing systems onto a common network of neural structures, regardless of how spoken words are represented orthographically in a writing system. During fun...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rueckl JG,Paz-Alonso PM,Molfese PJ,Kuo WJ,Bick A,Frost SJ,Hancock R,Wu DH,Mencl WE,Duñabeitia JA,Lee JR,Oliver M,Zevin JD,Hoeft F,Carreiras M,Tzeng OJ,Pugh KR,Frost R

更新日期：2015-12-15 00:00:00

abstract：:In the developing cerebellum, switching of the subunit composition of NMDA receptors occurs in granule cells from NR2B-containing receptors to NR2C-containing ones. We investigated the mechanisms underlying switching of NR2B and NR2C subunit composition in primary cultures of mouse granule cells at the physiological K...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Iijima K,Abe H,Okazawa M,Moriyoshi K,Nakanishi S

更新日期：2008-08-19 00:00:00

abstract：:Little is known about stem cell biology or the specialized environments or niches believed to control stem cell renewal and differentiation in self-renewing tissues of the body. Functional assays for stem cells are available only for hematopoiesis and spermatogenesis, and the microenvironment, or niche, for hematopoie...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shinohara T,Orwig KE,Avarbock MR,Brinster RL

更新日期：2001-05-22 00:00:00

abstract：:Several recombinant cowpox viruses were constructed and used to identify a viral gene that controls the production of hemorrhage in lesions caused by the Brighton Red strain of cowpox virus (CPV-BR). This gene is located in the KpnD fragment of CPV-BR DNA, between 31 and 32 kilobases from the end of the genome. This p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pickup DJ,Ink BS,Hu W,Ray CA,Joklik WK

更新日期：1986-10-01 00:00:00

abstract：:As the Conus shell grows by spiraling of the outer lip around the axis, profound internal shell dissolution thins the walls of the protected penultimate whorl from several millimeters to <50mum. Shell material is added to the inside of the spire and the anterior part of the columella. The resulting shell has a uniform...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kohn AJ,Myers ER,Meenakshi VR

更新日期：1979-07-01 00:00:00

abstract：:The allocation of carbon and nitrogen resources to the synthesis of plant proteins, carbohydrates, and lipids is complex and under the control of many genes; much remains to be understood about this process. QQS (Qua-Quine Starch; At3g30720), an orphan gene unique to Arabidopsis thaliana, regulates metabolic processes...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li L,Zheng W,Zhu Y,Ye H,Tang B,Arendsee ZW,Jones D,Li R,Ortiz D,Zhao X,Du C,Nettleton D,Scott MP,Salas-Fernandez MG,Yin Y,Wurtele ES

更新日期：2015-11-24 00:00:00

abstract：:Functional activity of N-methyl-D-aspartate (NMDA) receptors requires both glutamate binding and the binding of an endogenous coagonist that has been presumed to be glycine, although D-serine is a more potent agonist. Localizations of D-serine and it biosynthetic enzyme serine racemase approximate the distribution of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mothet JP,Parent AT,Wolosker H,Brady RO Jr,Linden DJ,Ferris CD,Rogawski MA,Snyder SH

更新日期：2000-04-25 00:00:00

abstract：:From a genomic library constructed from sperm DNA of the inbred BALB/c mouse, we previously isolated 54 cosmid clones that contain 36 class I genes and can be divided by restriction map analyses into 13 gene clusters. We have isolated single- and low-copy DNA probes from each of these clusters to visualize restriction...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Winoto A,Steinmetz M,Hood L

更新日期：1983-06-01 00:00:00

abstract：:A mammalian A-type cyclin, cyclin A1, is highly expressed in testes of both human and mouse and targeted mutagenesis in the mouse has revealed the unique requirement for cyclin A1 in the progression of male germ cells through the meiotic cell cycle. While very low levels of cyclin A1 have been reported in the human he...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liao C,Wang XY,Wei HQ,Li SQ,Merghoub T,Pandolfi PP,Wolgemuth DJ

更新日期：2001-06-05 00:00:00

abstract：:Cell cycle checkpoints are evolutionarily conserved surveillance systems that protect genomic stability and prevent oncogenesis in mammals. One important target of checkpoint control is ribonucleotide reductase (RNR), which catalyzes the rate-limiting step in dNTP and DNA synthesis. In both yeast and humans, RNR is tr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhao X,Rothstein R

更新日期：2002-03-19 00:00:00

abstract：:Maintenance of genomic stability is essential for normal organismal development and is vital to prevent diseases such as cancer. As genetic information is packaged into chromatin, it has become increasingly clear that the chromatin environment plays an important role in DNA damage response. However, how DNA repair is ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li X,Liu L,Yang S,Song N,Zhou X,Gao J,Yu N,Shan L,Wang Q,Liang J,Xuan C,Wang Y,Shang Y,Shi L

更新日期：2014-05-13 00:00:00