Regulation of a metallothionein-growth hormone hybrid gene in bovine papilloma virus.

abstract：:Tyrosine hydroxylase (EC 1.14.16.2), the enzyme catalyzing the rate-limiting step in catecholamine biosynthesis, was localized by electron microscopy within noradrenergic neurons of the nucleus locus coeruleus of the rat brain with a specific antibody to tyrosine hydroxylase labeled by the peroxidase-antiperoxidase im...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pickel VM,Joh TH,Reis DJ

更新日期：1975-02-01 00:00:00

abstract：:A mutant factor IX, designated factor IXCambridge, was isolated from a patient with hemophilia B. This protein includes an 18-residue propeptide attached to the NH2 terminus of factor IX. A point mutation at residue -1, from an arginine to a serine, precludes cleavage of the propeptide by a processing protease and int...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Diuguid DL,Rabiet MJ,Furie BC,Liebman HA,Furie B

更新日期：1986-08-01 00:00:00

abstract：:Antibodies specific for allelic determinants of the cell membrane alloantigen Ly-17 were found to react with genetically determined polymorphic sites on the murine IgG Fc receptor (Fc gamma R). Monoclonal antibodies specific for Ly-17.2 and the Fc gamma R detected identical populations of cells in thymus, spleen, lymp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Holmes KL,Palfree RG,Hammerling U,Morse HC 3rd

更新日期：1985-11-01 00:00:00

abstract：:p19ARF has been implicated as a key regulator of p53 stability and activation. While numerous stresses activate the p53 growth arrest pathway, those requiring p19ARF remain to be elucidated. We used p19ARF knockout mouse embryo fibroblasts to show that DNA damage and microtubule disruption require p19ARF to induce p53...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Khan SH,Moritsugu J,Wahl GM

更新日期：2000-03-28 00:00:00

abstract：:The ability of cocaine to inhibit the dopamine transporter (DAT) appears to be crucial for its reinforcing properties. The potential use of drugs that produce long-lasting inhibition of the DAT as a mean of preventing the "high" and reducing drug-seeking behavior has become a major strategy in medication development. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Volkow ND,Wang GJ,Fowler JS,Gatley SJ,Ding YS,Logan J,Dewey SL,Hitzemann R,Lieberman J

更新日期：1996-09-17 00:00:00

abstract：:Human herpesvirus 6 (HHV-6) like other herpesviruses, expresses sequentially immediate early (IE), early, and late genes during lytic infection. Evidence of ability to establish latent infection has not been available, but by analogy with other herpesviruses it could be expected that IE genes that regulate and transac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rotola A,Ravaioli T,Gonelli A,Dewhurst S,Cassai E,Di Luca D

更新日期：1998-11-10 00:00:00

abstract：:Insulin-degrading enzyme (IDE) selectively degrades the monomer of amyloidogenic peptides and contributes to clearance of amyloid β (Aβ). Thus, IDE retards the progression of Alzheimer's disease. IDE possesses an enclosed catalytic chamber that engulfs and degrades its peptide substrates; however, the molecular mechan...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McCord LA,Liang WG,Dowdell E,Kalas V,Hoey RJ,Koide A,Koide S,Tang WJ

更新日期：2013-08-20 00:00:00

abstract：:To identify the binding domain for dihydropyridine Ca2+ antagonists, skeletal muscle Ca2+ channels were photolabeled with [3H](+)-PN200-110 and [3H]azidopine. Regions of alpha 1 photolabeled by these ligands were then identified by antibody mapping of proteolytic fragments. Approximately 50% of the specific labeling b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Striessnig J,Murphy BJ,Catterall WA

更新日期：1991-12-01 00:00:00

abstract：:We have studied hepatic uptake of chylomicron retinyl ester. Chylomicrons were obtained from intestinal lymph of rats that were given retinol in groundnut oil by intraduodenal injection. When lymph was injected intravenously into normal rats, the radioactivity was cleared from blood with t1/2 approximately equal to 10...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Blomhoff R,Helgerud P,Rasmussen M,Berg T,Norum KR

更新日期：1982-12-01 00:00:00

abstract：:Cytochrome c maturation in Escherichia coli requires the ccm operon, which encodes eight membrane proteins (CcmABCDEFGH). CcmE is a periplasmic heme chaperone that binds heme covalently and transfers it onto apocytochrome c in the presence of CcmF, CcmG, and CcmH. In this work we addressed the functions of the ccmABCD...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schulz H,Fabianek RA,Pellicioli EC,Hennecke H,Thöny-Meyer L

更新日期：1999-05-25 00:00:00

abstract：:Intracellular transport is based on molecular motors that pull cargos along cytoskeletal filaments. One motor species always moves in one direction, e.g., conventional kinesin moves to the microtubule plus end, whereas cytoplasmic dynein moves to the microtubule minus end. However, many cellular cargoes are observed t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Müller MJ,Klumpp S,Lipowsky R

更新日期：2008-03-25 00:00:00

abstract：:Hybridoma technology was used for preparation of murine monoclonal antibodies of high titer against bone-Gla protein and osteonectin. A procedure of immunization and hybridization similar to that already described [Katzmann, J.A., Nesheim, M.E., Hibbard, L.S. & Mann, K.G. (1981) Proc. Natl. Acad. Sci. USA 78, 162-166;...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stenner DD,Romberg RW,Tracy RP,Katzmann JA,Riggs BL,Mann KG

更新日期：1984-05-01 00:00:00

abstract：:The proapoptotic activity of the "BH3-only" molecule BAD can be differentially regulated by survival factor signaling. Bad-deficient mice lacking both BAD long and BAD short proteins proved viable, and most cell types appeared to develop normally. BAD did not exclusively account for cell death after withdrawal of surv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ranger AM,Zha J,Harada H,Datta SR,Danial NN,Gilmore AP,Kutok JL,Le Beau MM,Greenberg ME,Korsmeyer SJ

更新日期：2003-08-05 00:00:00

abstract：:Complementation of the plasmin fragments of reduced-carbamoylmethylated (Cam) human somatotropin (hGH) with those of reduced-carbamoylmethylated human chorionic somatomammotropin (hCS) have been investigated. It was found that the recombinant obtained by noncovalent interaction of [Cys(Cam)53]hGH-(1-134) with [Cys(Cam...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li CH

更新日期：1978-04-01 00:00:00

abstract：:Probasin, a rat prostatic protein, is statistically related to members of a protein family that includes serum, cellular, and nuclear proteins. In vivo, probasin appears both in the secretions and in the nucleus of prostatic epithelial cells. Using primer extension and S1 nuclease protection assays we detected only on...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spence AM,Sheppard PC,Davie JR,Matuo Y,Nishi N,McKeehan WL,Dodd JG,Matusik RJ

更新日期：1989-10-01 00:00:00

abstract：:Life in environments devoid of photosynthesis, such as on early Earth or in contemporary dark subsurface ecosystems, is supported by chemical energy. How, when, and where chemical nutrients released from the geosphere fuel chemosynthetic biospheres is fundamental to understanding the distribution and diversity of life...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dunham EC,Dore JE,Skidmore ML,Roden EE,Boyd ES

更新日期：2021-01-12 00:00:00

abstract：:In this paper is presented the theory of a class of locally convex lattices (L-lattices) of real functions which generalize the classical Lebesgue spaces. The monotone and dominated convergence theorems for convergence almost everywhere and sequential and order completeness of such lattices are established. These resu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bogdanowicz WM

更新日期：1970-06-01 00:00:00

abstract：:Society makes substantial investments in biomedical research, searching for ways to better human health. The product of this research is principally information published in scientific journals. Continued investment in science relies on society's confidence in the accuracy, honesty, and utility of research results. A ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bowen A,Casadevall A

更新日期：2015-09-08 00:00:00

abstract：:We constructed a recombinant DNA molecule carrying the coding region and the 3' flanking sequences of the rat preproinsulin I gene, replacing the late region of a simian virus 40 (SV40) viral vector. The sequences making up the single intron of the preproinsulin I gene, located within the nontranslated leader region, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gruss P,Efstratiadis A,Karathanasis S,König M,Khoury G

更新日期：1981-10-01 00:00:00

abstract：:We present a model describing how Mg(2+) binds and stabilizes specific RNA structures. In this model, RNA stabilization arises from two energetically distinct modes of Mg(2+) binding: diffuse- and site-binding. Diffusely bound Mg(2+) are electrostatically attracted to the strong anionic field around the RNA and are ac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Misra VK,Draper DE

更新日期：2001-10-23 00:00:00

abstract：:Human infants are predisposed to rapidly acquire their native language. The nature of these predispositions is poorly understood, but is crucial to our understanding of how infants unpack their speech input to recover the fundamental word-like units, assign them referential roles, and acquire the rules that govern the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shukla M,White KS,Aslin RN

更新日期：2011-04-12 00:00:00

abstract：:Maintaining a fleet of buses to transport students to school is a major expense for school districts. To reduce costs by reusing buses between schools, many districts spread start times across the morning. However, assigning each school a time involves estimating the impact on transportation costs and reconciling addi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bertsimas D,Delarue A,Martin S

更新日期：2019-03-26 00:00:00

abstract：:The introduction of foreign genes into early mouse embryos and embryonic stem (ES) cells is invaluable for the analysis of gene function and regulation in the living animal. The use of vectors derived from retroviruses as gene transfer vehicles in this setting has had limited success because of silencing of transgene ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pfeifer A,Ikawa M,Dayn Y,Verma IM

更新日期：2002-02-19 00:00:00

abstract：:In the hippocampus, a CA3 pyramidal cell forms excitatory synapses with thousands of other pyramidal cells and inhibitory interneurons. By using sequential paired recordings from three connected cells, we show that the presynaptic properties of CA3 pyramidal cell terminals, belonging to the same axon, differ according...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Scanziani M,Gähwiler BH,Charpak S

更新日期：1998-09-29 00:00:00

abstract：:Purified cellular retinol-binding protein (CRBP), a potential mediator of vitamin A action, was found to enable retinol to bind in a specific manner to isolated nuclei from livers of vitamin A deficient rats. Binding was followed after complexing [3H]retinol with CRBP. The binding was specific, saturable, and temperat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Takase S,Ong DE,Chytil F

更新日期：1979-05-01 00:00:00

abstract：:A 43-kDa peripheral membrane protein is known to copurify with acetylcholine receptor (AcChoR)-rich membranes isolated from the electric organ of Torpedo californica. Immunoelectron microscopy and crosslinking studies have demonstrated that this 43-kDa protein is closely associated with the cytoplasmic domain(s) of th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Burden SJ

更新日期：1985-12-01 00:00:00

abstract：:Antigenic variation of African trypanosomes results from the periodic activation of a single new variant cell surface glycoprotein (VSG) gene out of a repertoire of about a 1000 VSG genes. In spite of the apparently random genetic basis of the process of antigenic variation, the relapsing parasitemias are characterize...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Agur Z,Abiri D,Van der Ploeg LH

更新日期：1989-12-01 00:00:00

abstract：:Depression, the most prevalent mental illness, is underdiagnosed and undertreated, highlighting the need to extend the scope of current screening methods. Here, we use language from Facebook posts of consenting individuals to predict depression recorded in electronic medical records. We accessed the history of Faceboo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eichstaedt JC,Smith RJ,Merchant RM,Ungar LH,Crutchley P,Preoţiuc-Pietro D,Asch DA,Schwartz HA

更新日期：2018-10-30 00:00:00

abstract：:The mechanism of the proline-catalyzed aldol reaction has stimulated considerable debate, and despite limited experimental data, at least five different mechanisms have been proposed. Complementary to recent theoretical studies we have initiated an experimental program with the goal of clarifying some of the basic mec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： List B,Hoang L,Martin HJ

更新日期：2004-04-20 00:00:00

abstract：:Zn2+ is critical for the functional and structural integrity of cells and contributes to a number of important processes including gene expression. It has been shown that NO exogenously applied via NO donors resulting in nitrosative stress leads to cytoplasmic Zn2+ release from the zinc storing protein metallothionein...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spahl DU,Berendji-Grün D,Suschek CV,Kolb-Bachofen V,Kröncke KD

更新日期：2003-11-25 00:00:00