Abstract:
:We constructed a recombinant DNA molecule carrying the coding region and the 3' flanking sequences of the rat preproinsulin I gene, replacing the late region of a simian virus 40 (SV40) viral vector. The sequences making up the single intron of the preproinsulin I gene, located within the nontranslated leader region, as well as all of the 5' flanking sequences, have been deleted. The sequences remaining within the late transcriptional unit of SV40 do not retain a functional intron. This "intronless" mutant nevertheless directs the synthesis of substantial quantities of unspliced stable hybrid transcripts that are translated into polypeptides with insulin immunoreactivity. Two novel 5' termini, not used in wild-type SV40 transcripts, have been detected in the majority of the hybrid RNA molecules. These are located at analogous positions in both of the late SV40 72-base-pair repeats.
journal_name
Proc Natl Acad Sci U S Aauthors
Gruss P,Efstratiadis A,Karathanasis S,König M,Khoury Gdoi
10.1073/pnas.78.10.6091subject
Has Abstractpub_date
1981-10-01 00:00:00pages
6091-5issue
10eissn
0027-8424issn
1091-6490journal_volume
78pub_type
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