Adaptive evolution of genomically recoded Escherichia coli.

abstract：:WOPR-domain proteins are found throughout the fungal kingdom where they function as master regulators of cell morphology and pathogenesis. Genetic and biochemical experiments previously demonstrated that these proteins bind to specific DNA sequences and thereby regulate transcription. However, their primary sequence s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lohse MB,Rosenberg OS,Cox JS,Stroud RM,Finer-Moore JS,Johnson AD

更新日期：2014-07-22 00:00:00

abstract：:Herpesvirus saimiri (HVS) is the prototype member of a distinctive subset of lymphotropic herpesviruses (the gamma 2 subgroup) with A+T-rich coding sequences. In this paper, we show that cells productively infected with HVS contain high concentrations of a virus-specified thymidylate synthase (5,10-methylenetetrahydro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Honess RW,Bodemer W,Cameron KR,Niller HH,Fleckenstein B,Randall RE

更新日期：1986-06-01 00:00:00

abstract：:Tumor growth requires neoangiogenesis. VEGF is the most potent proangiogenic factor. Dysregulation of hypoxia-inducible factor (HIF) or cytokine stimuli such as those involving the chemokine receptor 4/stromal-derived cell factor 1 (CXCR4/SDF-1) axis are the major cause of ectopic overexpression of VEGF in tumors. Alt...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： de Nigris F,Crudele V,Giovane A,Casamassimi A,Giordano A,Garban HJ,Cacciatore F,Pentimalli F,Marquez-Garban DC,Petrillo A,Cito L,Sommese L,Fiore A,Petrillo M,Siani A,Barbieri A,Arra C,Rengo F,Hayashi T,Al-Omran M,

更新日期：2010-08-10 00:00:00

abstract：:Synaptically released glutamate has been identified as a signal coupling excitatory neuronal activity to increased glucose utilization. The proposed mechanism of this coupling involves glutamate uptake into astrocytes resulting in increased intracellular Na+ (Nai+) and activation of the Na+/K+-ATPase. Increased metabo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chatton JY,Pellerin L,Magistretti PJ

更新日期：2003-10-14 00:00:00

abstract：:Tiazofurin (2-beta-D-ribofuranosyl-4-thiazole-carboxamide; NSC 286193), an antitumor carbon-linked nucleoside that inhibits IMP dehydrogenase (IMP:NAD+ oxidoreductase; EC 1.1.1.205) and depletes guanylate levels, can activate the erythroid differentiation program of K-562 human leukemia cells. Tiazofurin-mediated cell...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Olah E,Natsumeda Y,Ikegami T,Kote Z,Horanyi M,Szelenyi J,Paulik E,Kremmer T,Hollan SR,Sugar J

更新日期：1988-09-01 00:00:00

abstract：:Explaining the maintenance of tropical forest diversity under the countervailing forces of drift and competition poses a major challenge to ecological theory. Janzen-Connell effects, in which host-specific natural enemies restrict the recruitment of juveniles near conspecific adults, provide a potential mechanism. Jan...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Levi T,Barfield M,Barrantes S,Sullivan C,Holt RD,Terborgh J

更新日期：2019-01-08 00:00:00

abstract：:Eleven amino acid positions in the helix-turn-helix of lambda repressor have been mutagenized by using a combinatorial method in which alanine is substituted at each position with a probability of 0.5. Approximately 25% of the 2048 proteins in the resulting binomial library are active, including some variants with as ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gregoret LM,Sauer RT

更新日期：1993-05-01 00:00:00

abstract：:The 2.1-A resolution crystal structure of wild-type green fluorescent protein and comparison of it with the recently determined structure of the Ser-65 --> Thr (S65T) mutant explains the dual wavelength absorption and photoisomerization properties of the wild-type protein. The two absorption maxima are caused by a cha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brejc K,Sixma TK,Kitts PA,Kain SR,Tsien RY,Ormö M,Remington SJ

更新日期：1997-03-18 00:00:00

abstract：:The density of benzodiazepine/gamma-aminobutyric acid receptor binding sites was lower in the midbrain of seizure-susceptible gerbils compared to control seizure-resistant gerbils. Binding of [3H]diazepam to high-affinity brain-specific sites in membrane homogenates of gerbil brain showed a 20-30% lower binding in mid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Olsen RW,Wamsley JK,McCabe RT,Lee RJ,Lomax P

更新日期：1985-10-01 00:00:00

abstract：:Following carcinogen exposure in vitro, normal rat tracheal epithelial cells are transformed in a multistage process in which the cultured cells become immortal and, ultimately, neoplastic. Five cell lines derived from tumors produced by neoplastically transformed rat tracheal epithelial cells were examined for the ex...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Walker C,Nettesheim P,Barrett JC,Gilmer TM

更新日期：1987-04-01 00:00:00

abstract：:Assessing temporal variability in extreme rainfall events before the historical era is complicated by the sparsity of long-term "direct" storm proxies. Here we present a 2,200-y-long, accurate, and precisely dated record of cave flooding events from the northwest Australian tropics that we interpret, based on an integ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Denniston RF,Villarini G,Gonzales AN,Wyrwoll KH,Polyak VJ,Ummenhofer CC,Lachniet MS,Wanamaker AD Jr,Humphreys WF,Woods D,Cugley J

更新日期：2015-04-14 00:00:00

abstract：:Human infections with Ebola virus (EBOV) result in a deadly viral disease known as Ebola hemorrhagic fever. Up to 90% of infected patients die, and there is no available treatment or vaccine. The sporadic human outbreaks are believed to result when EBOV "jumps" from an infected animal to a person and is subsequently t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Strong JE,Wong G,Jones SE,Grolla A,Theriault S,Kobinger GP,Feldmann H

更新日期：2008-11-18 00:00:00

abstract：:A major challenge in interpreting genome sequences is understanding how the genome encodes the information that specifies when and where a gene will be expressed. The first step in this process is the identification of regions of the genome that contain regulatory information. In higher eukaryotes, this cis-regulatory...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berman BP,Nibu Y,Pfeiffer BD,Tomancak P,Celniker SE,Levine M,Rubin GM,Eisen MB

更新日期：2002-01-22 00:00:00

abstract：:Alzheimer's disease (AD) is an irreversible neurodegenerative disorder that has a progression that is closely associated with oxidative stress. It has long been speculated that the reactive oxygen species (ROS) level in AD brains is much higher than that in healthy brains. However, evidence from living beings is scarc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang J,Zhang X,Yuan P,Yang J,Xu Y,Grutzendler J,Shao Y,Moore A,Ran C

更新日期：2017-11-21 00:00:00

abstract：:The first step in the formation of the protease resistant form (PrPSc) of prion proteins involves a conformational transition of the monomeric cellular form of PrPC to a more stable aggregation prone state PrPC*. A search of PDBselect and Escherichia coli and yeast genomes shows that the exact pattern of charges in he...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dima RI,Thirumalai D

更新日期：2004-10-26 00:00:00

abstract：:When a mutant of Saccharomyces cerevisiae (spe2 delta) that cannot make spermidine or spermine was incubated in a polyamine-deficient medium in oxygen, there was a rapid cessation of cell growth and associated cell death. In contrast, when the mutant cells were incubated in the polyamine-deficient medium in air or ana...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Balasundaram D,Tabor CW,Tabor H

更新日期：1993-05-15 00:00:00

abstract：:Two clusters of configurations of the main proteolytic subunit β5 were identified by principal component analysis of crystal structures of the yeast proteasome core particle (yCP). The apo-cluster encompasses unliganded species and complexes with nonpeptidic ligands, and the pep-cluster comprises complexes with peptid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Arciniega M,Beck P,Lange OF,Groll M,Huber R

更新日期：2014-07-01 00:00:00

abstract：:Public responses where identical T cell receptors (TCRs) are clonally dominant and shared between different individuals are a common characteristic of CD8(+) T cell-mediated immunity. Focusing on TCR sharing, we analyzed approximately 3,400 TCR beta chains (TCRbetas) from mouse CD8(+) T cells responding to the influen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Venturi V,Kedzierska K,Price DA,Doherty PC,Douek DC,Turner SJ,Davenport MP

更新日期：2006-12-05 00:00:00

abstract：:Cross-talk between the microtubule and actin networks has come under intense scrutiny following the realization that it is crucial for numerous essential processes, ranging from cytokinesis to cell migration. It is becoming increasingly clear that proteins long-considered highly specific for one or the other cytoskele...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Oberhofer A,Reithmann E,Spieler P,Stepp WL,Zimmermann D,Schmid B,Frey E,Ökten Z

更新日期：2020-02-25 00:00:00

abstract：:Methane can be converted to triose dihydroxyacetone (DHA) by chemical processes with formaldehyde as an intermediate. Carbon dioxide, a by-product of various industries including ethanol/butanol biorefineries, can also be converted to formaldehyde and then to DHA. DHA, upon entry into a cell and phosphorylation to DHA...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang L,Chauliac D,Rhee MS,Panneerselvam A,Ingram LO,Shanmugam KT

更新日期：2018-04-24 00:00:00

abstract：:Evidence from more than 100 y of research indicates that conscientiousness (C) is the most potent noncognitive construct for occupational performance. However, questions remain about the magnitudes of its effect sizes across occupational variables, its defining characteristics and functions in occupational settings, a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wilmot MP,Ones DS

更新日期：2019-11-12 00:00:00

abstract：:Experimental and computational simulations revealed that boron clusters, which favor planar (2D) structures up to 18 atoms, prefer 3D structures beginning at 20 atoms. Using global optimization methods, we found that the B20 neutral cluster has a double-ring tubular structure with a diameter of 5.2 A. For the B(-)20 a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kiran B,Bulusu S,Zhai HJ,Yoo S,Zeng XC,Wang LS

更新日期：2005-01-25 00:00:00

abstract：:Infection is a major complication of implantable medical devices, which provide a scaffold for biofilm formation, thereby reducing susceptibility to antibiotics and complicating treatment. Hematogenous implant-related infections following bacteremia are particularly problematic because they can occur at any time in a ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang Y,Cheng LI,Helfer DR,Ashbaugh AG,Miller RJ,Tzomides AJ,Thompson JM,Ortines RV,Tsai AS,Liu H,Dillen CA,Archer NK,Cohen TS,Tkaczyk C,Stover CK,Sellman BR,Miller LS

更新日期：2017-06-27 00:00:00

abstract：:IL-17 is a T cell-derived, proinflammatory cytokine that is suspected to be involved in the development of various inflammatory diseases. Although there are elevated levels of IL-17 in synovial fluid of patients with rheumatoid arthritis, the pathogenic role of IL-17 in the development of rheumatoid arthritis remains ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nakae S,Saijo S,Horai R,Sudo K,Mori S,Iwakura Y

更新日期：2003-05-13 00:00:00

abstract：:Limited trypsinization of the fatty acid synthetase multienzyme complex from rat mammary gland results in the release of a protein, molecular weight 32,000, with thioesterase activity. The other components of the multienzyme complex--the acyl carrier protein, acetyl and malonyl transferases, condensing enzyme, keto re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith S,Agradi E,Libertini L,Dileepan KN

更新日期：1976-04-01 00:00:00

abstract：:In nine patients with GM1 gangliosidosis, liver ganglioside GM1 beta-galactosidase (EC 3.2.1.23) activity ranged from less than 0.01% to 0.05% of normal. In a tenth patient's liver, much higher activity was found (0.5% of normal). In this patient the residual enzyme had the same molecular weight as beta-galactosidase ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Norden AG,O'Brien JS

更新日期：1975-01-01 00:00:00

abstract：:The CD8 dimer interacts with the alpha 3 domain of major histocompatibility complex class I molecules through two immunoglobulin variable-like domains. In this study a crystal structure-informed mutational analysis has been performed to identify amino acids in the CD8 alpha/alpha homodimer that are likely to be involv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Giblin PA,Leahy DJ,Mennone J,Kavathas PB

更新日期：1994-03-01 00:00:00

abstract：:O-GlcNAc glycosylation (or O-GlcNAcylation) is a dynamic, inducible posttranslational modification found on proteins associated with neurodegenerative diseases such as α-synuclein, amyloid precursor protein, and tau. Deletion of the O-GlcNAc transferase (ogt) gene responsible for the modification causes early postnata...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang AC,Jensen EH,Rexach JE,Vinters HV,Hsieh-Wilson LC

更新日期：2016-12-27 00:00:00

abstract：:Murine monoclonal antibodies that bind selectively to adducts formed on DNA by the antitumor drug cis-diamminedichloroplatinum(II), cis-DDP, or to the chemotherapeutically inactive trans isomer trans-DDP were elicited by immunization with calf thymus DNA modified with either cis- or trans-DDP at ratios of bound platin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sundquist WI,Lippard SJ,Stollar BD

更新日期：1987-12-01 00:00:00

abstract：:It was recently demonstrated that amyloid beta (Abeta) peptide vaccination was effective in reducing the Abeta burden in Alzheimer model mice. However, the clinical trial was halted because of the development of meningoencephalitis in some patients. To overcome this problem, anti-Abeta antibody therapy and other types...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Okura Y,Miyakoshi A,Kohyama K,Park IK,Staufenbiel M,Matsumoto Y

更新日期：2006-06-20 00:00:00