Abstract：
:Our study was designed to investigate the role of B4GalT1 in glioblastoma, in vitro and in vivo, to detect whether B4GalT1 knockdown could regulate the development of glioblastoma, and further observe the relationship between B4GalT1 knockdown and the apoptosis and autophagy of glioblastoma. To begin, we looked at TCGA and GEPIA systems to predict the potential function of B4GalT1. Western blot and RT-PCR were used to analyze the expression, or mRNA level, of B4GalT1 at different tissue or cell lines. Next, the occurrence and development of glioblastoma, in vitro and in vivo, was observed by using B4GalT1 knocked down by lentivirus. Finally, the apoptosis and autophagy of glioblastoma was observed in vitro and in vivo. Results show that B4GalT1 was a highly variable gene, and GEPIA and TCGA systems show B4GalT1 expression in GBM tumor tissue was higher than in normal tissue. Pair-wise gene correlation analysis revealed a probable relationship between B4GalT1 and autophagy related proteins. The B4GalT1 expression and mRNA level were increased in tumor cells, or U87 cells. B4GalT1 knocked down by lentivirus could inhibit glioblastoma development, in vitro and in vivo, by reducing tumor weight and volume, increasing survival, and weakening tumor cells proliferation, migration, invasion. B4GalT1 knockdown could increase apoptosis and autophagy of glioblastoma in vitro and in vivo. Our study demonstrates that B4GalT1 may be able to regulate apoptosis and autophagy of glioblastoma. Bax, Bcl-2, cleaved caspase-3, Beclin-1, and LC3 s may be the downstream target factors of B4GalT1 in apoptosis and autophagy, which may provide a new strategy to reduce glioblastoma development by regulating apoptosis and autophagy.
journal_name
Technol Cancer Res Treatjournal_title
Technology in cancer research & treatmentauthors
Wang P,Li X,Xie Ydoi
10.1177/1533033820980104subject
Has Abstractpub_date
2020-01-01 00:00:00pages
1533033820980104eissn
1533-0346issn
1533-0338journal_volume
19pub_type
杂志文章abstract：:We have constructed a multimodal contrast agent suitable for near-infrared, NIR, fluorescent imaging as well as magnetic resonance imaging, MRI. This class of agents may be useful for preoperative tumor localization and tumor functional evaluation and for intraoperative delineation of tumor margins. We have covalently...
journal_title：Technology in cancer research & treatment
pub_type： 杂志文章
doi：10.1177/153303460600500401
更新日期：2006-08-01 00:00:00
abstract：:A review of the literature on the current applications of breast magnetic resonance imaging (MRI) indications, their rationale and their place in diagnosis and management of breast cancer was given. Contrast-enhanced breast MRI is developing as a valuable adjunct to mammography and sonography. Its high sensitivity for...
journal_title：Technology in cancer research & treatment
pub_type： 杂志文章,评审
doi：10.1177/153303460400300602
更新日期：2004-12-01 00:00:00
abstract：:Spinal metastases are a common manifestation of malignant tumors that can cause severe pain, spinal cord compression, pathological fractures, and hypercalcemia, and these clinical manifestations will ultimately reduce the health-related quality of life and even shorten life expectancy in patient with cancer. Effective...
journal_title：Technology in cancer research & treatment
pub_type： 杂志文章
doi：10.1177/1533033820945798
更新日期：2020-01-01 00:00:00
abstract：PURPOSE:The study was aimed to compare online 6 degree-of-freedom image registrations of TrueBeam cone-beam computed tomography and BrainLab ExacTrac X-ray imaging systems for intracranial radiosurgery. METHODS:Phantom and patient studies were performed on a Varian TrueBeam STx linear accelerator (version 2.5), which ...
journal_title：Technology in cancer research & treatment
pub_type： 杂志文章
doi：10.1177/1533034616683069
更新日期：2017-06-01 00:00:00
abstract：:The coupling mechanism between neuronal firing and cerebrovascular dilatation can be significantly compromised in cerebral diseases, making it difficult to identify eloquent cortical areas near or within resectable lesions by using Blood Oxygen Level Dependent (BOLD) fMRI. Several metabolic and vascular factors have ...
journal_title：Technology in cancer research & treatment
pub_type： 杂志文章
doi：10.7785/tcrt.2012.500284
更新日期：2012-08-01 00:00:00
abstract：INTRODUCTION:Rectal cancer ranks as the eighth in cancer-related morbidity and the tenth in the cancer-related mortality. A few studies have explored several biomarkers for colorectal cancer. However, there is still a great need for us to excavate novel biomarkers with effective and efficient diagnostic and prognostic ...
journal_title：Technology in cancer research & treatment
pub_type： 杂志文章
doi：10.1177/1533033820973270
更新日期：2020-01-01 00:00:00
abstract：:Most experimental studies of electroporation focus on permeabilization of the outer cell membrane. Some experiments address delivery of ions and molecules into cells that should survive; others focus on efficient killing of the cells with minimal temperature rise. A basic method for quantifying electroporation effecti...
journal_title：Technology in cancer research & treatment
pub_type： 杂志文章
doi：10.1177/1533033818792491
更新日期：2018-01-01 00:00:00
abstract：:The aim of this study is to evaluate the volume differences between contrast-enhanced CT-based left ventricle (LV) and PET-CT-based LV and assess the impact of dose on the substructure volume differences in patients with left breast cancer treated with adjuvant radiotherapy. From October 2008 to February 2009, 14 pati...
journal_title：Technology in cancer research & treatment
pub_type： 杂志文章
doi：10.7785/tcrt.2012.500299
更新日期：2013-04-01 00:00:00
abstract：:Optical mammography is one of several new techniques for breast cancer detection and characterization presently under development for clinical use that provide information other than morphologic, in particular on the biochemical and metabolic state of normal and diseased tissue. In breast tissue, scattering of red to ...
journal_title：Technology in cancer research & treatment
pub_type： 杂志文章
doi：10.1177/153303460500400503
更新日期：2005-10-01 00:00:00
abstract：:A 51 year old male with a history of right facial numbness developed progressive upper lip swelling for one year, but an MRI of the head was unremarkable. A wide local excision of the upper lip was performed and pathology revealed a 1.7 cm mass, poorly differentiated squamous cell carcinoma with perineural invasion. S...
journal_title：Technology in cancer research & treatment
pub_type： 杂志文章
doi：10.1177/153303460500400304
更新日期：2005-06-01 00:00:00
abstract：:The PIN diode developed by Bhabha Atomic Research Centre (BARC) was modified for its use as a dosimeter in radiation therapy. For this purpose the diode was mounted on a printed circuit board (PCB) and provided with necessary connections so that its response against irradiation can be recorded by a standard radiothera...
journal_title：Technology in cancer research & treatment
pub_type： 杂志文章
doi：10.7785/tcrt.2012.500388
更新日期：2014-08-01 00:00:00
abstract：:Fascin is overexpressed in various tumor tissues and is closely related to tumor metastasis and invasion. However, the role of fascin in cholangiocarcinoma RBE cells has not been clearly reported. This study aimed to establish a cholangiocarcinoma cell line with stable and high expression of fascin to observe the effe...
journal_title：Technology in cancer research & treatment
pub_type： 杂志文章
doi：10.1177/1533034615580696
更新日期：2016-04-01 00:00:00
abstract：:The efficacy of photodynamic therapy for the treatment of a large number of skin diseases, in particular non-malignant skin cancer, has been reported by a number of studies. It has been demonstrated to be particularly beneficial for large or multiple lesions, especially where good cosmesis is required. However for PDT...
journal_title：Technology in cancer research & treatment
pub_type： 杂志文章,评审
doi：10.1177/153303460300200402
更新日期：2003-08-01 00:00:00
abstract：BACKGROUND:Testicular cancer represents the most common malignancy in young adult men. In the current study, we sought to analyze and compare the prognostic value of lymph node ratio (LNR) as well as positive lymph node counts (LNC) to understand its clinical significance in testicular germ cell tumors. METHODS:We emp...
journal_title：Technology in cancer research & treatment
pub_type： 杂志文章
doi：10.1177/1533033820979702
更新日期：2021-01-01 00:00:00
abstract：:This study quantifies the setup uncertainties to optimize the planning target volume (PTV) margin based on daily image guidance, its dosimetric impact, and radiobiological implication for intensity-modulated radiation therapy (IMRT) in head and neck cancer. Ten patients were retrospectively chosen who had been treated...
journal_title：Technology in cancer research & treatment
pub_type： 杂志文章
doi：10.1177/1533034614561162
更新日期：2016-02-01 00:00:00
abstract：:We describe the first histopathologic analysis of prostatic tissue following hypofractionated robotic radiation therapy. A 66 year-old man presented with stage II, low risk adenocarcinoma of the prostate and underwent elective conformal hypofractionated radiation therapy. His pretreatment evaluation revealed T1c adeno...
journal_title：Technology in cancer research & treatment
pub_type： 杂志文章
doi：10.1177/153303461000900606
更新日期：2010-12-01 00:00:00
abstract：OBJECTIVE:To evaluate and quantify the planning performance of automatic planning (AP) with manual planning (MP) for nasopharyngeal carcinoma in the RayStation treatment planning system (TPS). METHODS:A progressive and effective design method for AP of nasopharyngeal carcinoma was realized through automated scripts in...
journal_title：Technology in cancer research & treatment
pub_type： 杂志文章
doi：10.1177/1533033820915710
更新日期：2020-01-01 00:00:00
abstract：:Hepatocellular carcinoma (HCC) is the most aggressive type of gastrointestinal tumor, with a high rate of mortality. However, identifying biomarkers for the treatment of HCC remains to be developed. We aimed to determine whether cell division cycle 25C (CDC25C) could be used as a novel diagnostic and therapeutic bioma...
journal_title：Technology in cancer research & treatment
pub_type： 杂志文章
doi：10.1177/1533033820967474
更新日期：2020-01-01 00:00:00
abstract：:The aim of this paper is to study the feasibility for determining the individual internal target volume (ITV) for hepatocellular carcinoma (HCC) using 3DCT associated with active breathing coordinator (ABC), comparing the ITVs defined by 3DCT associated with ABC and 4DCT. Thirteen patients with HCC after transarterial...
journal_title：Technology in cancer research & treatment
pub_type： 杂志文章
doi：10.1177/153303461101000610
更新日期：2011-12-01 00:00:00
abstract：:Mesenchymal stromal cells are proven to be likely induce the angiogenic response in multiple myeloma and thus represent an enticing target for antiangiogenesis therapies for multiple myeloma. Substantial evidence indicates that angiogenesis in multiple myeloma is complex and involves direct production of angiogenic cy...
journal_title：Technology in cancer research & treatment
pub_type： 杂志文章
doi：10.1177/1533033818764473
更新日期：2018-01-01 00:00:00
abstract：:MicroRNAs are reported as a vital important factor in cancer cell initiation and progression processes. MicroRNA-19-3p has drawn the attention of many researchers in recent years because of its wide expression and its key role in serious kinds of tumor cells. However, the detailed mechanism of microRNA-19a-3p in these...
journal_title：Technology in cancer research & treatment
pub_type： 杂志文章
doi：10.1177/1533033820917978
更新日期：2020-01-01 00:00:00
abstract：:The inhibitor of growth (ING) family proteins have been defined as candidate tumor suppressors. ING4 as a novel member of ING family has potential tumor-suppressive effects via multiple pathways. However, the therapeutic effect of adenovirus-mediated ING4 (Ad-ING4) gene transfer in human osteosarcoma is still unknown....
journal_title：Technology in cancer research & treatment
pub_type： 杂志文章
doi：10.7785/tcrt.2012.500424
更新日期：2015-10-01 00:00:00
abstract：:Breast cancer remains one of the leading causes of cancer-associated death in women. MiR-27a is highly expressed in breast cancer tissue. However, the underlying mechanisms that promote breast cancer progression are unknown. In this study, we investigated the regulatory mechanisms of miR-27a and its target glycogen Sy...
journal_title：Technology in cancer research & treatment
pub_type： 杂志文章
doi：10.1177/1533033820965576
更新日期：2020-01-01 00:00:00
abstract：:We investigated the technique, early results and toxicity of curative stereotactic radiotherapy with the CyberKnife (Accuray Incorporated, Sunnyvale, California, USA) in 15 extracranial, extrapulmonary, extrahepatic, and extraspinal tumors. Fourteen tumors were located close to the bowel or esophagus. The PTV = GTV + ...
journal_title：Technology in cancer research & treatment
pub_type： 杂志文章
doi：10.1177/153303460700600603
更新日期：2007-12-01 00:00:00
abstract：:Traditional imaging techniques, such as computed tomography (CT), have high spatial resolution for changes in the anatomy or structure of organs, but are not very useful for detecting early biochemical changes. Conversely, positron emission tomography (PET) relies on changes in molecular biology to identify the earlie...
journal_title：Technology in cancer research & treatment
pub_type： 杂志文章,评审
doi：10.1177/153303460600500204
更新日期：2006-04-01 00:00:00
abstract：:There are many motivations for adding simultaneously acquired MR images to PET scanning. The most straight forward are, superior registration of MR and PET images, the addition of morphological detail when there is non-rigid motion and for pre-clinical studies simultaneous imaging could lead to a significant reduction...
journal_title：Technology in cancer research & treatment
pub_type： 杂志文章
doi：10.1177/153303461000900106
更新日期：2010-02-01 00:00:00
abstract：:Optical methods such as reflectance and fluorescence spectroscopy are being investigated for their potential to aid cancer detection in a quantitative, minimally invasive manner. Mathematical models of reflectance and fluorescence provide an important link between measured optical data and biomedically-relevant tissue...
journal_title：Technology in cancer research & treatment
pub_type： 杂志文章,评审
doi：10.7785/tcrt.2012.500187
更新日期：2011-04-01 00:00:00
abstract：:The purpose of this study was to evaluate the feasibility and treatment plans of intensity-modulated radiation therapy using helical tomotherapy (HT) for brain metastases. Twenty-three patients with 1 to 4 brain metastases were treated with HT. In combination with whole-brain radiotherapy (simultaneous plans), metasta...
journal_title：Technology in cancer research & treatment
pub_type： 杂志文章
doi：10.1177/153303460800700602
更新日期：2008-12-01 00:00:00
abstract：:We have developed a small animal conformal radiation therapy device that provides a degree of geometrical/anatomical targeting comparable to what is achievable in a commercial animal irradiator. small animal conformal radiation therapy device is capable of producing precise and accurate conformal delivery of radiation...
journal_title：Technology in cancer research & treatment
pub_type： 杂志文章
doi：10.1177/1533034615626011
更新日期：2017-02-01 00:00:00
abstract：:Many conventional chemotherapeutic drugs, as well as DNA for cancer gene therapy, require efficient access to the cell interior to be effective. The cell membrane is a formidable barrier to many of these drugs, including therapeutic DNA constructs. Electropermeabilization (EP, often used synonymously with "electropora...
journal_title：Technology in cancer research & treatment
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1177/153303460200100110
更新日期：2002-02-01 00:00:00
