Abstract:
:Bariatric surgery is the most effective treatment for weight loss. Vertical sleeve gastrectomy (VSG) involves the resection of ~ 80% of the stomach and was conceived to purely restrict oral intake. However, evidence suggests more complex mechanisms, particularly postoperative changes in gut microbiota, in facilitating weight loss and resolving associated comorbidities. VSG in humans is a complex procedure and includes peri-operative antibiotics and caloric restriction in addition to the altered anatomy. The impact of each of these factors on the intestinal microbiota have not been evaluated. The aim of this study was to determine the relative contributions of each of these factors on intestinal microbiota composition following VSG prior to substantial weight loss. Thirty-two obese patients underwent one of three treatments: (1) VSG plus routine intravenous peri-operative antibiotics (n = 12), (2) VSG with intravenous vancomycin chosen for its low intestinal penetrance (n = 12), and (3) caloric restriction (n = 8). Fecal samples were evaluated for bacterial composition prior to and 7 days following each intervention. Only patients undergoing VSG with routine peri-operative antibiotics showed a significant shift in community composition. Our data support the single dose of routine peri-operative antibiotics as the most influential factor of intestinal microbial composition acutely following VSG.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Nalluri H,Kizy S,Ewing K,Luthra G,Leslie DB,Bernlohr DA,Sadowsky MJ,Ikramuddin S,Khoruts A,Staley C,Jahansouz Cdoi
10.1038/s41598-020-77285-7subject
Has Abstractpub_date
2020-11-23 00:00:00pages
20340issue
1issn
2045-2322pii
10.1038/s41598-020-77285-7journal_volume
10pub_type
杂志文章abstract::Hepatitis B virus (HBV) infection is a leading cause of hepatocellular carcinoma worldwide. However, the strategy of HBV to escape from the host immune system remains largely unknown. In this study, we examined extracellular vesicles (EVs) secreted from human hepatocytes infected with HBV. EVs includeing exosomes are ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-07853-x
更新日期:2017-08-10 00:00:00
abstract::Polycystic ovary syndrome (PCOS) is characterized by failure of ovulation and is associated with obesity and chronic inflammation. Recent evidence suggests that anomalous activation of ovarian macrophages and numerical and functional deficits in the Th17 (CD4+IL17A+) and the CD4+CD25+CD127low Tregs plays crucial role ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-42960-x
更新日期:2019-04-25 00:00:00
abstract::Barrett's esophagus (BE) is the only known precursor to esophageal adenocarcinoma (EAC). Methods of identifying BE patients at high risk for progression to high-grade dysplasia (HGD) or EAC are needed to improve outcomes and identify who will benefit most from intensive surveillance or ablative therapy. Clinical predi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-61874-7
更新日期:2020-03-17 00:00:00
abstract::Mucosal-associated invariant T (MAIT) cells help combat opportunistic infections. Thus, MAIT cells are of interest in HIV/SIV vaccination and infection. We investigated MAIT cell dynamics and function in rhesus macaque blood and bronchoalveolar lavage (BAL) following mucosal adenovirus (Ad)-SIV recombinant priming, in...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-66964-0
更新日期:2020-06-22 00:00:00
abstract::Seasonal influenza virus infections cause hundreds of thousands of deaths annually while viral mutation raises the threat of a novel pandemic strain. Antiviral drugs exhibit limited efficacy unless administered early and may induce viral resistance. Thus, targeting the host response directly has been proposed as a nov...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11030
更新日期:2015-06-05 00:00:00
abstract::BMPs are multifunctional growth factors implicated in regulating the ovarian function as key intra-ovarian factors. Biological effects of BMPs are mediated through binding with membrane bound receptors like BMPR-IB and initiating downstream Smad signaling pathway. FecB mutation, regarded as a loss of function mutation...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-77596-9
更新日期:2020-11-24 00:00:00
abstract::Epithelial-to-mesenchymal transition (EMT) is a biological process in which epithelial cells translate into a mesenchymal phenotype with invasive capacities, contributing to tumour progression, metastasis, and the acquisition of chemotherapy resistance. To identify new therapeutic targets for cancers, it is important ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-16555-3
更新日期:2017-11-27 00:00:00
abstract::Phytoplankton subsumes the great variety of unicellular photoautotrophs that perform roughly half of Earth's primary production. They achieve this despite their challenging oceanic habitat, with opposing vertical gradients of nutrients (which often limit their growth near the surface) and light (which becomes limiting...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-57890-2
更新日期:2020-01-24 00:00:00
abstract::We aimed to model longitudinal data to create predictive growth charts for weight in preterm infants from birth till discharge, that took into account the differing growth rates post-birth when compared to in-utero growth and therefore was more representative of the data than the UK1990 reference charts. Data from bir...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-60895-6
更新日期:2020-03-04 00:00:00
abstract::Saliva, as a non-invasive and easily accessible biofluid, has been shown to contain RNA biomarkers for prediction and diagnosis of several diseases. However, systematic analysis done by our group identified two problematic issues not coherently described before: (1) most of the isolated RNA originates from the oral mi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-67825-6
更新日期:2020-07-07 00:00:00
abstract::We examined whether blood lead levels (BLLs) were associated with reproductive hormone levels in a cross-sectional study using data from the SPECT-China study. We selected 2286 men and 1571 postmenopausal women without hormone replacement therapy. BLLs, blood cadmium, total testosterone (TT), oestradiol (E2), luteiniz...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep37809
更新日期:2016-11-29 00:00:00
abstract::Human female germline stem cells (FGSCs) have opened new opportunities for understanding human oogenesis, delaying menopause, treating infertility, and providing a new strategy for preserving fertility. However, the shortage of adult human ovaries tissues available impedes their future investigations and clinical appl...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep28218
更新日期:2016-06-30 00:00:00
abstract::Energy transfer upconversion (ETU) is known to be the most efficient frequency upconversion mechanism. Surface plasmon can further enhance the upconversion process, opening doors to many applications. However, ETU is a complex process involving competing transitions between multiple energy levels and it has been diffi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep18894
更新日期:2016-01-07 00:00:00
abstract::Competition between simultaneously presented visual stimuli lengthens reaction time and reduces both the BOLD response and neural firing. In contrast, conditions of sequential presentation have been assumed to be free from competition. Here we manipulated the spatial proximity of stimuli (Near versus Far conditions) t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-05011-x
更新日期:2017-07-06 00:00:00
abstract::Active deep-sea hydrothermal vents harbor abundant thermophilic and hyperthermophilic microorganisms. However, microbial communities in inactive hydrothermal vents have not been well documented. Here, we investigated bacterial and archaeal communities in the two deep-sea sediments (named as TVG4 and TVG11) collected f...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25982
更新日期:2016-05-12 00:00:00
abstract::Heat stress threatens agriculture worldwide. Plants acquire heat stress tolerance through priming, which establishes stress memory during mild or severe transient heat stress. Such induced thermotolerance restructures metabolic networks and helps maintain metabolic homeostasis under heat stress. Here, we used an elect...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-36484-z
更新日期:2019-01-17 00:00:00
abstract::Defense mechanisms of plant genomes can epigenetically inactivate repetitive sequences and exogenous transgenes. Loss of mutant phenotypes in intronic T-DNA insertion lines by interaction with another T-DNA locus, termed T-DNA suppression, has been observed in Arabidopsis thaliana, although the molecular basis of esta...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep45166
更新日期:2017-03-24 00:00:00
abstract::Vascular endothelial growth factor receptor 2 (VEGFR2) is a potential cell-type biomarker in clinical diagnoses. Besides, it's the target protein of many tyrosine kinase inhibitors and its expression significantly associates with clinical performance of these inhibitors. VEGFR2 detection provides an early warning for ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep03982
更新日期:2014-02-05 00:00:00
abstract::Multi-phenotype analysis has drawn increasing attention to high-throughput genomic studies, whereas only a few applications have justified the use of multivariate techniques. We applied a recently developed multi-trait analysis method on a small set of bacteria hypersensitive response phenotypes and identified a singl...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep45281
更新日期:2017-03-24 00:00:00
abstract::IncX4 plasmids are associated with the dissemination of the mcr-1 genes in Enterobacteriaceae. We screened IncX4 plasmids among 2,470 isolates of Enterobacteriaceae and determined the mcr-1 positive isolates. Forty-three isolates were observed to carry IncX4 type plasmid, among which 13 were identified to carry mcr-1 ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-00095-x
更新日期:2017-03-24 00:00:00
abstract::Thrombin-induced and proteinase-activated receptor 1 (PAR1)-mediated signaling increases ROS production, activates ERK, and promotes inflammation and fibroblast proliferation in bleomycin-induced lung injury. Stanniocalcin-1 (STC1) activates anti-oxidant pathways, inhibits inflammation and provides cytoprotection; hen...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep18117
更新日期:2015-12-07 00:00:00
abstract::Attention can be directed at features and feature dimensions to facilitate perception. Here, we investigated whether feature-based-attention (FBA) can also dynamically weight feature-specific representations within multi-feature objects held in visual working memory (VWM). Across three experiments, participants retain...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep42384
更新日期:2017-02-24 00:00:00
abstract::Alternative oxidase (AOX) is a mitochondrial inner-membrane oxidase that accepts electrons directly from ubiquinol and reduces oxygen to water without involving cytochrome-linked electron transport chain. It is highly conserved in many non-vertebrate taxa and may protect cells against hypoxia and oxidative stress. We ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-10976-w
更新日期:2017-09-07 00:00:00
abstract::Microsphere-assisted microscopy currently benefits from a considerable interest in the microscope-research community. Indeed, this new imaging technique enables the lateral resolution of optical microscopes to reach around λ/5 through a full-field and a far-field acquisition while being label-free. Despite the photoni...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-41193-2
更新日期:2019-03-18 00:00:00
abstract::Salinity is a critical abiotic stress, which significantly impacts the agricultural yield worldwide. Identification of the molecular mechanisms underlying the salt tolerance in euhalophyte Suaeda salsa is conducive to the development of salt-resistant crops. In the present study, high-throughput RNA sequencing was per...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-61204-x
更新日期:2020-03-06 00:00:00
abstract::This study aimed to evaluate the application value of diffusion-weighted imaging (DWI) for assessing paradoxical puborectalis syndrome (PPS) in patients with obstructive defecation syndrome (ODS). The medical records of 72 ODS patients who underwent magnetic resonance (MR)-DWI and MR-defecography were retrospectively ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-03127-8
更新日期:2017-06-07 00:00:00
abstract::The combination of genome-edited human embryonic stem cells (hESCs) and subsequent neural differentiation is a powerful tool to study neurodevelopmental disorders. Since the naïve state of pluripotency has favourable characteristics for efficient genome-editing, we optimized a workflow for the CRISPR/Cas9 system in th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-16932-y
更新日期:2017-11-30 00:00:00
abstract::Seagrass meadows are threatened by coastal development and global change. In the face of these pressures, molecular techniques such as reverse transcription quantitative real-time PCR (RT-qPCR) have great potential to improve management of these ecosystems by allowing early detection of chronic stress. In RT-qPCR, the...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep17051
更新日期:2015-11-23 00:00:00
abstract::The autonomic nervous system (ANS) regulates tissue homeostasis and remodelling through antagonistic effects of noradrenergic sympathetic and cholinergic parasympathetic signalling. Despite numerous reports on the induction of sympathetic neurons from human pluripotent stem cells (hPSCs), no induction methods have eff...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-66303-3
更新日期:2020-06-11 00:00:00
abstract::Hepatic metabolic stability is a key pharmacokinetic parameter in drug discovery. Metabolic stability is usually assessed in microsomal fractions and only the best compounds progress in the drug discovery process. A high-throughput single time point substrate depletion assay in rat liver microsomes (RLM) is employed a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-77327-0
更新日期:2020-11-26 00:00:00