Aberrant expression of semaphorin 6B affects cell phenotypes in thyroid carcinoma by activating Notch signaling pathway.

Abstract:

:Numerous members of semaphorins have been widely clarified to be involved in the development of multiple cancers. However, the semaphorin 6B (SEMA6B) has not yet been extensively reported in cancers, especially in thyroid carcinoma. According to the public dataset from TCGA database, we found that the expression of SEMA6B was upregulated in thyroid carcinoma tissues and high level of SEMA6B resulted in a poorer prognosis. Functional experiments showed that silencing SEMA6B suppressed B-CPAP cells viability, invasiveness and motility, whereas up-regulating SEMA6B in FTC-133 cells led to opposite outcomes. Furthermore, knockdown of SEMA6B in B-CPAP cells could significantly elevate the protein expression of NUMB and reduce the expression of NOTCH1, HES1, and Cyclin D1. Overexpression of SEMA6B in FTC-133 cells presented the inverse results. These findings demonstrated that SEMA6B exerts a tumorigenic effect in thyroid carcinoma by activating Notch signaling pathway, which provide a possible biomarker for thyroid carcinoma.

journal_name

Endokrynol Pol

journal_title

Endokrynologia Polska

authors

Lv XJ,Chen X,Wang Y,Yu S,Pang L,Huang C

doi

10.5603/EP.a2020.0072

subject

Has Abstract

pub_date

2020-10-30 00:00:00

eissn

0423-104X

issn

2299-8306

pii

VM/OJS/J/68545

pub_type

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