A novel gingipain regulatory gene in Porphyromonas gingivalis mediates host cell detachment and inhibition of wound closure.

Abstract:

:The black pigmentation-related genes in Porphyromonas gingivalis are primarily involved in regulating gingipain functions. In this study, we identified a pigmentation-related gene, designated as pgn_0361. To characterize the role of pgn_0361 in regulating P. gingivalis-mediated epithelial cell detachment and inhibition of wound closure, PgΔ0361, an isogenic pgn_0361-defective mutant strain, and PgΔ0361C, a complementation strain, were constructed using P. gingivalis ATCC 33277. The gingipain and hemagglutination activities, as well as biofilm formation, were examined in all three strains. The effect of P. gingivalis strains on epithelial cell detachment was investigated using the HO-1-N-1 and Ca9-22 epithelial cell lines. The inhibition of wound closure by heat-killed P. gingivalis cells and culture supernatant was analyzed using an in vitro wound closure assay. Compared to the wild-type strain, the PgΔ0361 strain did not exhibit gingipain or hemagglutination activity but exhibited enhanced biofilm formation. Additionally, the PgΔ0361 strain exhibited attenuated ability to detach the epithelial cells and to inhibit wound closure in vitro. Contrastingly, the culture supernatant of PgΔ0361 exhibited high gingipain activity and strong inhibition of wound closure. The characteristics of PgΔ0361C and wild-type strains were comparable. In conclusion, the pgn_0361 gene is involved in regulating gingipains. The PGN_0361-defective strain exhibited reduced virulence in terms of epithelial cell detachment and inhibition of wound closure. The culture supernatant of the mutant strain highly inhibited wound closure, which may be due to high gingipain activity.

journal_name

Microbiologyopen

journal_title

MicrobiologyOpen

authors

Liu H,Huang L,Cai Y,Bikker FJ,Wei X,Mei Deng D

doi

10.1002/mbo3.1128

subject

Has Abstract

pub_date

2020-12-01 00:00:00

pages

e1128

issue

12

issn

2045-8827

journal_volume

9

pub_type

杂志文章
  • Mapping and determinism of soil microbial community distribution across an agricultural landscape.

    abstract::Despite the relevance of landscape, regarding the spatial patterning of microbial communities and the relative influence of environmental parameters versus human activities, few investigations have been conducted at this scale. Here, we used a systematic grid to characterize the distribution of soil microbial communit...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.255

    authors: Constancias F,Terrat S,Saby NP,Horrigue W,Villerd J,Guillemin JP,Biju-Duval L,Nowak V,Dequiedt S,Ranjard L,Chemidlin Prévost-Bouré N

    更新日期:2015-06-01 00:00:00

  • Insights into gene regulation of the halovirus His2 infecting Haloarcula hispanica.

    abstract::Gene expression in Haloarcula hispanica cells infected with the gammapleolipovirus His2 was studied using a custom DNA microarray. Total RNA from cells sampled at 0, 1, 2, 3, and 4.5 hr postinfection was reverse-transcribed into labeled cDNA and hybridized to microarrays, revealing temporal and differential expression...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.1016

    authors: Lee STM,Ding JY,Chiang PW,Dyall-Smith M,Tang SL

    更新日期:2020-05-01 00:00:00

  • Response of the rhizosphere prokaryotic community of barley (Hordeum vulgare L.) to elevated atmospheric CO2 concentration in open-top chambers.

    abstract::The effect of elevated atmospheric CO2 concentration [CO2 ] on the diversity and composition of the prokaryotic community inhabiting the rhizosphere of winter barley (Hordeum vulgare L.) was investigated in a field experiment, using open-top chambers. Rhizosphere samples were collected at anthesis (flowering stage) fr...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.462

    authors: Szoboszlay M,Näther A,Mitterbauer E,Bender J,Weigel HJ,Tebbe CC

    更新日期:2017-08-01 00:00:00

  • Identification and characterization of OmpT-like proteases in uropathogenic Escherichia coli clinical isolates.

    abstract::Bacterial colonization of the urogenital tract is limited by innate defenses, including the production of antimicrobial peptides (AMPs). Uropathogenic Escherichia coli (UPEC) resist AMP-killing to cause a range of urinary tract infections (UTIs) including asymptomatic bacteriuria, cystitis, pyelonephritis, and sepsis....

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.915

    authors: Desloges I,Taylor JA,Leclerc JM,Brannon JR,Portt A,Spencer JD,Dewar K,Marczynski GT,Manges A,Gruenheid S,Le Moual H,Thomassin JL

    更新日期:2019-11-01 00:00:00

  • AcuC, a histone deacetylase, contributes to the pathogenicity of Aeromonas hydrophila.

    abstract::The interactions of pathogens and phagocytes are complex. Our study demonstrated that Aeromonas hydrophila B11 can survive in the macrophagocytes of Tilapia mossambica. To explore the regulatory processes of A. hydrophila survival in the macrophagocytes, we used the mini-Tn10 transposon mutagenesis system to build a m...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.468

    authors: Jiang Q,Chen W,Qin Y,Huang L,Xu X,Zhao L,Yan Q

    更新日期:2017-08-01 00:00:00

  • Genomic integration of unclonable gene expression cassettes in Saccharomyces cerevisiae using rapid cloning-free workflows.

    abstract::Most DNA assembly methods require bacterial amplification steps, which restrict its application to genes that can be cloned in the bacterial host without significant toxic effects. However, genes that cannot be cloned in bacteria do not necessarily exert toxic effects on the final host. In order to tackle this issue, ...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.978

    authors: Cataldo VF,Salgado V,Saa PA,Agosin E

    更新日期:2020-03-01 00:00:00

  • Nutrient availability in tropical caves influences the dynamics of microbial biomass.

    abstract::Few studies have evaluated the trophic level in tropical caves, and none related the microbial biomass dynamics in the immobilization of carbon and nitrogen. Here, four tropical caves of Terra Ronca State Park, Brazil, were studied: Angélica, São Bernardo, Terra Ronca I, and Terra Ronca II caves. Physical, chemical, a...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.1044

    authors: Paula CCP,Bichuette ME,Seleghim MHR

    更新日期:2020-07-01 00:00:00

  • Co-dynamics of Symbiodiniaceae and bacterial populations during the first year of symbiosis with Acropora tenuis juveniles.

    abstract::Interactions between corals and their associated microbial communities (Symbiodiniaceae and prokaryotes) are key to understanding corals' potential for and rate of acclimatory and adaptive responses. However, the establishment of microalgal and bacterial communities is poorly understood during coral ontogeny in the wi...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.959

    authors: Quigley KM,Alvarez Roa C,Torda G,Bourne DG,Willis BL

    更新日期:2020-02-01 00:00:00

  • Depletion of acidic phospholipids influences chromosomal replication in Escherichia coli.

    abstract::In Escherichia coli, coordinated activation and deactivation of DnaA allows for proper timing of the initiation of chromosomal synthesis at the origin of replication (oriC) and assures initiation occurs once per cell cycle. In vitro, acidic phospholipids reactivate DnaA, and in vivo depletion of acidic phospholipids, ...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.46

    authors: Fingland N,Flåtten I,Downey CD,Fossum-Raunehaug S,Skarstad K,Crooke E

    更新日期:2012-12-01 00:00:00

  • Swimming and rafting of E.coli microcolonies at air-liquid interfaces.

    abstract::The dynamics of swimming microorganisms is strongly affected by solid-liquid and air-liquid interfaces. In this paper, we characterize the motion of both single bacteria and microcolonies at an air-liquid interface. Both of them follow circular trajectories. Single bacteria preferentially show a counter-clockwise moti...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.532

    authors: Sinibaldi G,Iebba V,Chinappi M

    更新日期:2018-02-01 00:00:00

  • Evaluating bacterial community structures in oil collected from the sea surface and sediment in the northern Gulf of Mexico after the Deepwater Horizon oil spill.

    abstract::Bacterial community structures were evaluated in oil samples using culture-independent pyrosequencing, including oil mousses collected on sea surface and salt marshes during the Deepwater Horizon oil spill, and oil deposited in sediments adjacent to the wellhead 1 year after the spill. Phylogenetic analysis suggested ...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.89

    authors: Liu Z,Liu J

    更新日期:2013-06-01 00:00:00

  • Effect of two cosmetic compounds on the growth, biofilm formation activity, and surface properties of acneic strains of Cutibacterium acnes and Staphylococcus aureus.

    abstract::Increasing popularity of preservative-free cosmetics necessitates in-depth research, specifically as bacteria can react to local factors by important metabolic changes. In this respect, investigating the effect of cosmetic preparations on pathogenic strains of commensal species such as acneic forms of Cutibacterium ac...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.659

    authors: Gannesen AV,Borrel V,Lefeuvre L,Netrusov AI,Plakunov VK,Feuilloley MGJ

    更新日期:2019-03-01 00:00:00

  • A mutation in the promoter region of zipA, a component of the divisome, suppresses the shape defect of RodZ-deficient cells.

    abstract::RodZ is important for maintaining the rod shape of Escherichia coli. Loss of RodZ causes conversion of the rod shape to a round shape and a growth rate slower than that of wild-type cells. Suppressor mutations that simultaneously restore both the growth rates and the rod shape were isolated. Most of the suppressor mut...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.116

    authors: Shiomi D,Niki H

    更新日期:2013-10-01 00:00:00

  • Confocal laser scanning, scanning electron, and transmission electron microscopy investigation of Enterococcus faecalis biofilm degradation using passive and active sodium hypochlorite irrigation within a simulated root canal model.

    abstract::Root canal irrigation is an important adjunct to control microbial infection. The aim of this study was to investigate the effect of 2.5% (wt/vol) sodium hypochlorite (NaOCl) agitation on the removal, killing, and degradation of Enterococcus faecalis biofilm. A total of 45 root canal models were manufactured using 3D ...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.455

    authors: Mohmmed SA,Vianna ME,Penny MR,Hilton ST,Mordan N,Knowles JC

    更新日期:2017-08-01 00:00:00

  • Comparative genomics of hepatitis A virus, hepatitis C virus, and hepatitis E virus provides insights into the evolutionary history of Hepatovirus species.

    abstract::The intraspecies genomic diversity of the single-strand RNA (+) virus species hepatitis A virus (Hepatovirus), hepatitis C virus (Hepacivirus), and hepatitis E virus (Orthohepevirus) was compared. These viral species all can cause liver inflammation (hepatitis), but share no gene similarity. The codon usage of human h...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.973

    authors: Wassenaar TM,Jun SR,Robeson M,Ussery DW

    更新日期:2020-02-01 00:00:00

  • Temporal transcriptomes of a marine cyanopodovirus and its Synechococcus host during infection.

    abstract::Marine picocyanobacteria belonging to genera Synechococcus and Prochlorococcus are genetically diverged and distributed into distinct biogeographical patterns, and both are infected by genetically closely related cyanopodoviruses. Previous studies have not fully explored whether the two virus-host systems share simila...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.1150

    authors: Huang S,Sun Y,Zhang S,Long L

    更新日期:2020-12-30 00:00:00

  • CopM is a novel copper-binding protein involved in copper resistance in Synechocystis sp. PCC 6803.

    abstract::Copper resistance system in the cyanobacterium Synechocystis sp. PCC 6803 comprises two operons, copMRS and copBAC, which are expressed in response to copper in the media. copBAC codes for a heavy-metal efflux-resistance nodulation and division (HME-RND) system, while copMRS codes for a protein of unknown function, Co...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.231

    authors: Giner-Lamia J,López-Maury L,Florencio FJ

    更新日期:2015-02-01 00:00:00

  • UV-resistant yeasts isolated from a high-altitude volcanic area on the Atacama Desert as eukaryotic models for astrobiology.

    abstract::The Sairecabur volcano (5971 m), in the Atacama Desert, is a high-altitude extreme environment with high daily temperature variations, acidic soils, intense UV radiation, and low availability of water. Four different species of yeasts were isolated from this region using oligotrophic media, identified and characterize...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.262

    authors: Pulschen AA,Rodrigues F,Duarte RT,Araujo GG,Santiago IF,Paulino-Lima IG,Rosa CA,Kato MJ,Pellizari VH,Galante D

    更新日期:2015-08-01 00:00:00

  • New insights into FtsZ rearrangements during the cell division of Escherichia coli from single-molecule localization microscopy of fixed cells.

    abstract::FtsZ - a prokaryotic tubulin homolog - is one of the central components of bacterial division machinery. At the early stage of cytokinesis FtsZ forms the so-called Z-ring at mid-cell that guides septum formation. Many approaches were used to resolve the structure of the Z-ring, however, researchers are still far from ...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.336

    authors: Vedyaykin AD,Vishnyakov IE,Polinovskaya VS,Khodorkovskii MA,Sabantsev AV

    更新日期:2016-06-01 00:00:00

  • Comparative analysis of early immune responses induced by two strains of Newcastle disease virus in chickens.

    abstract::Newcastle disease, caused by virulent strain of Newcastle disease virus (NDV), is an acute, highly contagious disease that is prevalent worldwide and is responsible for serious economic losses to the poultry industry. In the current study, we compared the early immune responses in chickens infected with two strains of...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.701

    authors: Zhang T,Ren M,Liu C,Xu L,Wang F,Han Z,Shao Y,Ma D

    更新日期:2019-04-01 00:00:00

  • UV Resistance of bacteria from the Kenyan Marine cyanobacterium Moorea producens.

    abstract::UV resistance of bacteria isolated from the marine cyanobacterium Moorea producens has not been observed previously, findings which highlight how unsafe germicidal UV irradiation for sterilization of air, food, and water could be. Further, UV resistance of Bacillus licheniformis is being observed for the first time. T...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.697

    authors: Dzeha T,Nyiro C,Kardasopoulos D,Mburu D,Mwafaida J,Hall MJ,Burgess JG

    更新日期:2019-04-01 00:00:00

  • Evaluation of two xenobiotic reductases from Pseudomonas putida for their suitability for magnetic nanoparticle-directed enzyme prodrug therapy as a novel approach to cancer treatment.

    abstract::Directed enzyme prodrug therapy (DEPT) is a cancer chemotherapy strategy in which bacterial enzymes are delivered to a cancer site before prodrug administration, resulting in prodrug activation at the cancer site and more localized treatment. A major limitation to DEPT is the poor effectiveness of the most studied enz...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.1110

    authors: Ball P,Halliwell J,Anderson S,Gwenin V,Gwenin C

    更新日期:2020-10-01 00:00:00

  • Conservation and diversity of the IrrE/DdrO-controlled radiation response in radiation-resistant Deinococcus bacteria.

    abstract::The extreme radiation resistance of Deinococcus bacteria requires the radiation-stimulated cleavage of protein DdrO by a specific metalloprotease called IrrE. DdrO is the repressor of a predicted radiation/desiccation response (RDR) regulon, composed of radiation-induced genes having a conserved DNA motif (RDRM) in th...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.477

    authors: Blanchard L,Guérin P,Roche D,Cruveiller S,Pignol D,Vallenet D,Armengaud J,de Groot A

    更新日期:2017-08-01 00:00:00

  • Deep tillage combined with biofertilizer following soil fumigation improved chrysanthemum growth by regulating the soil microbiome.

    abstract::Sustained monoculture often leads to the inhibition of plant growth, the decrease of the soil microbial diversity, and changes in soil microbial community composition, particularly to the accumulation of soil-borne pathogens. In this study, we conducted field experiments to investigate the practical effects of tilling...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.1045

    authors: Chen H,Zhao S,Zhao J,Zhang K,Jiang J,Guan Z,Chen S,Chen F,Fang W

    更新日期:2020-07-01 00:00:00

  • Bacterial community dynamics in spontaneous sourdoughs made from wheat, spelt, and rye wholemeal flour.

    abstract::Sourdough fermentation is a traditional process that is used to improve bread quality. A spontaneous sourdough ecosystem consists of a mixture of flour and water that is fermented by endogenous lactic acid bacteria (LAB) and yeasts. The aim of this study was to identify bacterial diversity during backslopping of spont...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.1009

    authors: Boreczek J,Litwinek D,Żylińska-Urban J,Izak D,Buksa K,Gawor J,Gromadka R,Bardowski JK,Kowalczyk M

    更新日期:2020-04-01 00:00:00

  • The role of compatible solutes in desiccation resistance of Acinetobacter baumannii.

    abstract::Acinetobacter baumannii is a nosocomial pathogen which can persist in the hospital environment not only due to the acquirement of multiple antibiotic resistances, but also because of its exceptional resistance against disinfectants and desiccation. A suitable desiccation assay was established in which A. baumannii ATC...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.740

    authors: Zeidler S,Müller V

    更新日期:2019-05-01 00:00:00

  • Assessment of carbapenem-resistant Enterobacteriaceae-plate formula and quality control procedure.

    abstract:AIMS:To assess a cost-effective in-house selective plate formula for actively screening carbapenem-resistant Enterobacteriaceae (CRE). METHODOLOGY AND RESULTS:The in-house formula included CHROMagarTM Orientation, meropenem, and ingredients present in the Mac-Conkey formula, such as bile salts and crystal violet (pH 6...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.1130

    authors: Tran TC,Pham BT,Pham VH,Ngo TA,Hanberger H,Larsson M,Olson L

    更新日期:2020-12-01 00:00:00

  • The effects of low levels of trivalent ions on a standard strain of Escherichia coli (ATCC 11775) in aqueous solutions.

    abstract::Considering the ever-growing usage of trivalent salts in water treatment, for example, lanthanum salts in rare earth, AlCl3 and FeCl3 , the effects of different trivalent cations on the bacterium Escherichia coli (E. coli) ATCC 11775 strain have been studied in aqueous solutions. From colony incubation studies, the co...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.574

    authors: Deng C,Li X,Xue X,Pashley RM

    更新日期:2018-06-01 00:00:00

  • Gut microbiome diversity influenced more by the Westernized dietary regime than the body mass index as assessed using effect size statistic.

    abstract::Human gut microbiome dysbiosis has been associated with the onset of metabolic diseases and disorders. However, the critical factors leading to dysbiosis are poorly understood. In this study, we provide increasing evidence of the association of diet type and body mass index (BMI) and how they relatively influence the ...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.476

    authors: Davis SC,Yadav JS,Barrow SD,Robertson BK

    更新日期:2017-08-01 00:00:00

  • Antimicrobial activity and chemical composition of white birch (Betula papyrifera Marshall) bark extracts.

    abstract::Extracts from white birch have been reported to possess antimicrobial properties, but no study has linked the chemical composition of bark extract with antimicrobial activity. This study aimed to identify white birch (Betula papyrifera Marshall) bark extracts with antimicrobial activity and elucidate its composition. ...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.944

    authors: Blondeau D,St-Pierre A,Bourdeau N,Bley J,Lajeunesse A,Desgagné-Penix I

    更新日期:2020-01-01 00:00:00