Insights into gene regulation of the halovirus His2 infecting Haloarcula hispanica.

Abstract:

:Gene expression in Haloarcula hispanica cells infected with the gammapleolipovirus His2 was studied using a custom DNA microarray. Total RNA from cells sampled at 0, 1, 2, 3, and 4.5 hr postinfection was reverse-transcribed into labeled cDNA and hybridized to microarrays, revealing temporal and differential expression in both host and viral genes. His2 gene expression occurred in three main phases (early, middle, and late), and by 4.5 hr p.i. the majority of genes were actively transcribed, including those encoding the major structural proteins. Eighty host genes were differentially regulated ≥twofold postinfection, with most of them predicted to be involved in transport, translation, and metabolism. Differentially expressed host genes could also be grouped into early-, middle-, and late-expressed genes based on the timing of their up- and downregulation postinfection. The altered host transcriptional pattern suggests regulation by His2 infection, which may reprogram host metabolism to facilitate its own DNA replication and propagation. This study enhances the characterization of many hypothetical viral genes and provides insights into the interaction between His2 and its host.

journal_name

Microbiologyopen

journal_title

MicrobiologyOpen

authors

Lee STM,Ding JY,Chiang PW,Dyall-Smith M,Tang SL

doi

10.1002/mbo3.1016

subject

Has Abstract

pub_date

2020-05-01 00:00:00

pages

e1016

issue

5

issn

2045-8827

journal_volume

9

pub_type

杂志文章
  • The effect of sulfamonomethoxine treatment on the gut microbiota of Nile tilapia (Oreochromis niloticus).

    abstract::To investigate the possible effects of sulfamonomethoxine (SMM) on Nile tilapia (Oreochromis niloticus), we quantitatively evaluated the microbial shifts in the intestines of Nile tilapia in response to different doses of SMM (200 and 300 mg/kg) using 16S rRNA gene sequencing. At the phylum level, the control group (0...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.1116

    authors: Ming J,Fu Z,Ma Z,Zhou L,Zhang Z,Song C,Yuan X,Wu Q

    更新日期:2020-11-01 00:00:00

  • Selective chemoattraction of the benthic diatom Seminavis robusta to phosphate but not to inorganic nitrogen sources contributes to biofilm structuring.

    abstract::Diatoms frequently dominate marine and freshwater biofilms as major primary producers. Nutrient resources in these biofilms are patchily distributed and fluctuate dynamically over time. We recently reported that this spatially and temporally structured environment can be exploited by motile diatoms that use chemoattra...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.694

    authors: V Bondoc KG,Lembke C,Vyverman W,Pohnert G

    更新日期:2019-04-01 00:00:00

  • Genetic relatedness of the Escherichia coli fecal population and strains causing urinary tract infection in the same host.

    abstract::It is common knowledge that fecal microbiota is a primary source of Escherichia coli causing urinary tract infections (UTIs) via the fecal-perineal-urethral route. But, it is still unknown whether E. coli UTI is mainly caused by dominant fecal E. coli isolates (prevalence hypothesis) or the isolates that possess more ...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.759

    authors: Bahadori M,Motamedifar M,Derakhshandeh A,Firouzi R,Motamedi Boroojeni A,Alinejad M,Naziri Z

    更新日期:2019-06-01 00:00:00

  • Truncation of type IV pilin induces mucoidy in Pseudomonas aeruginosa strain PAO579.

    abstract::Pseudomonas aeruginosa is a Gram negative, opportunistic pathogen that uses the overproduction of alginate, a surface polysaccharide, to form biofilms in vivo. Overproduction of alginate, also known as mucoidy, affords the bacterium protection from the host's defenses and facilitates the establishment of chronic lung ...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.86

    authors: Ryan Withers T,Heath Damron F,Yin Y,Yu HD

    更新日期:2013-06-01 00:00:00

  • The effects of low levels of trivalent ions on a standard strain of Escherichia coli (ATCC 11775) in aqueous solutions.

    abstract::Considering the ever-growing usage of trivalent salts in water treatment, for example, lanthanum salts in rare earth, AlCl3 and FeCl3 , the effects of different trivalent cations on the bacterium Escherichia coli (E. coli) ATCC 11775 strain have been studied in aqueous solutions. From colony incubation studies, the co...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.574

    authors: Deng C,Li X,Xue X,Pashley RM

    更新日期:2018-06-01 00:00:00

  • Taxonomic and functional profiles of soil samples from Atlantic forest and Caatinga biomes in northeastern Brazil.

    abstract::Although microorganisms play crucial roles in ecosystems, metagenomic analyses of soil samples are quite scarce, especially in the Southern Hemisphere. In this work, the microbial diversity of soil samples from an Atlantic Forest and Caatinga was analyzed using a metagenomic approach. Proteobacteria and Actinobacteria...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.169

    authors: Pacchioni RG,Carvalho FM,Thompson CE,Faustino AL,Nicolini F,Pereira TS,Silva RC,Cantão ME,Gerber A,Vasconcelos AT,Agnez-Lima LF

    更新日期:2014-06-01 00:00:00

  • Conservation and diversity of the IrrE/DdrO-controlled radiation response in radiation-resistant Deinococcus bacteria.

    abstract::The extreme radiation resistance of Deinococcus bacteria requires the radiation-stimulated cleavage of protein DdrO by a specific metalloprotease called IrrE. DdrO is the repressor of a predicted radiation/desiccation response (RDR) regulon, composed of radiation-induced genes having a conserved DNA motif (RDRM) in th...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.477

    authors: Blanchard L,Guérin P,Roche D,Cruveiller S,Pignol D,Vallenet D,Armengaud J,de Groot A

    更新日期:2017-08-01 00:00:00

  • A mutation in the promoter region of zipA, a component of the divisome, suppresses the shape defect of RodZ-deficient cells.

    abstract::RodZ is important for maintaining the rod shape of Escherichia coli. Loss of RodZ causes conversion of the rod shape to a round shape and a growth rate slower than that of wild-type cells. Suppressor mutations that simultaneously restore both the growth rates and the rod shape were isolated. Most of the suppressor mut...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.116

    authors: Shiomi D,Niki H

    更新日期:2013-10-01 00:00:00

  • Response of the rhizosphere prokaryotic community of barley (Hordeum vulgare L.) to elevated atmospheric CO2 concentration in open-top chambers.

    abstract::The effect of elevated atmospheric CO2 concentration [CO2 ] on the diversity and composition of the prokaryotic community inhabiting the rhizosphere of winter barley (Hordeum vulgare L.) was investigated in a field experiment, using open-top chambers. Rhizosphere samples were collected at anthesis (flowering stage) fr...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.462

    authors: Szoboszlay M,Näther A,Mitterbauer E,Bender J,Weigel HJ,Tebbe CC

    更新日期:2017-08-01 00:00:00

  • Autoinducer2 affects trimethoprim-sulfamethoxazole susceptibility in avian pathogenic Escherichia coli dependent on the folate synthesis-associate pathway.

    abstract::Avian pathogenic Escherichia coli (APEC) causes airsacculitis, polyserositis, septicemia, and other mainly extraintestinal diseases in chickens, ducks, geese, pigeons, and other avian species, and is responsible for great economic losses in the avian industry. The autoinducer 2 (AI-2) quorum sensing system is widely p...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.582

    authors: Yu L,Li W,Zhang M,Cui Y,Chen X,Ni J,Yu L,Shang F,Xue T

    更新日期:2018-08-01 00:00:00

  • Swimming and rafting of E.coli microcolonies at air-liquid interfaces.

    abstract::The dynamics of swimming microorganisms is strongly affected by solid-liquid and air-liquid interfaces. In this paper, we characterize the motion of both single bacteria and microcolonies at an air-liquid interface. Both of them follow circular trajectories. Single bacteria preferentially show a counter-clockwise moti...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.532

    authors: Sinibaldi G,Iebba V,Chinappi M

    更新日期:2018-02-01 00:00:00

  • A putative merR family transcription factor Slr0701 regulates mercury inducible expression of MerA in the cyanobacterium Synechocystis sp. PCC6803.

    abstract::In cyanobacteria, genes conferring mercury resistance are not organized as mer-operon, unlike in other bacterial phyla. Synechocystis contains only a putative MerR regulator, Slr0701, and a mercury reductase, MerA, located aside from each other in the genome. The slr0701-mutant showed reduction in photosynthetic activ...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.838

    authors: Singh DK,Lingaswamy B,Koduru TN,Nagu PP,Jogadhenu PSS

    更新日期:2019-09-01 00:00:00

  • The role of compatible solutes in desiccation resistance of Acinetobacter baumannii.

    abstract::Acinetobacter baumannii is a nosocomial pathogen which can persist in the hospital environment not only due to the acquirement of multiple antibiotic resistances, but also because of its exceptional resistance against disinfectants and desiccation. A suitable desiccation assay was established in which A. baumannii ATC...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.740

    authors: Zeidler S,Müller V

    更新日期:2019-05-01 00:00:00

  • Phage therapy treatment of the coral pathogen Vibrio coralliilyticus.

    abstract::Vibrio coralliilyticus is an important coral pathogen demonstrated to cause disease outbreaks worldwide. This study investigated the feasibility of applying bacteriophage therapy to treat the coral pathogen V. coralliilyticus. A specific bacteriophage for V. coralliilyticus strain P1 (LMG23696), referred to here as ba...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.52

    authors: Cohen Y,Joseph Pollock F,Rosenberg E,Bourne DG

    更新日期:2013-02-01 00:00:00

  • Mcr colistin resistance gene: a systematic review of current diagnostics and detection methods.

    abstract::Resistance to colistin, mediated by chromosomal mutations and more recently, by plasmid-borne mcr genes, is increasingly being reported in bacterial isolates taken from humans, animals, farms, foods, and the environment. To easily identify and contain this quickly spreading menace, efficient diagnostics that are cheap...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.682

    authors: Osei Sekyere J

    更新日期:2019-04-01 00:00:00

  • Global transcriptome responses including small RNAs during mixed-species interactions with methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa.

    abstract::Pseudomonas aeruginosa and Staphylococcus aureus mixed-species biofilm infections are more resilient to biocide attacks compared to their single-species counterparts. Therefore, this study used an in vitro model recapitulating bacterial burdens seen in in vivo infections to investigate the interactions of P. aeruginos...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.427

    authors: Miller CL,Van Laar TA,Chen T,Karna SLR,Chen P,You T,Leung KP

    更新日期:2017-06-01 00:00:00

  • Exploring the directionality of Escherichia coli formate hydrogenlyase: a membrane-bound enzyme capable of fixing carbon dioxide to organic acid.

    abstract::During mixed-acid fermentation Escherichia coli produces formate, which is initially excreted out the cell. Accumulation of formate, and dropping extracellular pH, leads to biosynthesis of the formate hydrogenlyase (FHL) complex. FHL consists of membrane and soluble domains anchored within the inner membrane. The solu...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.365

    authors: Pinske C,Sargent F

    更新日期:2016-10-01 00:00:00

  • The Aeromonas caviae AHA0618 gene modulates cell length and influences swimming and swarming motility.

    abstract::Aeromonas caviae is motile via a polar flagellum in liquid culture, with a lateral flagella system used for swarming on solid surfaces. The polar flagellum also has a role in cellular adherence and biofilm formation. The two subunits of the polar flagellum, FlaA and FlaB, are posttranslationally modified by O-linked g...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.233

    authors: Lowry RC,Parker JL,Kumbhar R,Mesnage S,Shaw JG,Stafford GP

    更新日期:2015-04-01 00:00:00

  • Unique secreted-surface protein complex of Lactobacillus rhamnosus, identified by phage display.

    abstract::Proteins are the most diverse structures on bacterial surfaces; hence, they are candidates for species- and strain-specific interactions of bacteria with the host, environment, and other microorganisms. Genomics has decoded thousands of bacterial surface and secreted proteins, yet the function of most cannot be predic...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.53

    authors: Gagic D,Wen W,Collett MA,Rakonjac J

    更新日期:2013-02-01 00:00:00

  • Hexyl gallate for the control of citrus canker caused by Xanthomonas citri subsp citri.

    abstract::Brazil is the biggest producer of sweet oranges and the main exporter of concentrated orange juice in the world. Among the diseases that affect citriculture, Asiatic citrus canker, caused by the bacterial pathogen Xanthomonas citri, represents one of the most significant threats. The current Brazilian legislation regu...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.1104

    authors: Cavalca LB,Zamuner CFC,Saldanha LL,Polaquini CR,Regasini LO,Behlau F,Ferreira H

    更新日期:2020-09-01 00:00:00

  • Metabolic engineering to expand the substrate spectrum of Pseudomonas putida toward sucrose.

    abstract::Sucrose is an important disaccharide used as a substrate in many industrial applications. It is a major component of molasses, a cheap by-product of the sugar industry. Unfortunately, not all industrially relevant organisms, among them Pseudomonas putida, are capable of metabolizing sucrose. We chose a metabolic engin...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.473

    authors: Löwe H,Schmauder L,Hobmeier K,Kremling A,Pflüger-Grau K

    更新日期:2017-08-01 00:00:00

  • Bacterial community dynamics in spontaneous sourdoughs made from wheat, spelt, and rye wholemeal flour.

    abstract::Sourdough fermentation is a traditional process that is used to improve bread quality. A spontaneous sourdough ecosystem consists of a mixture of flour and water that is fermented by endogenous lactic acid bacteria (LAB) and yeasts. The aim of this study was to identify bacterial diversity during backslopping of spont...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.1009

    authors: Boreczek J,Litwinek D,Żylińska-Urban J,Izak D,Buksa K,Gawor J,Gromadka R,Bardowski JK,Kowalczyk M

    更新日期:2020-04-01 00:00:00

  • Spatial patterns in soil physicochemical and microbiological properties in a grassland adjacent to a newly built lake.

    abstract::Soil water content (SWC) is an important determinant for nutrient cycling and microorganism activity in the grassland ecosystem. Lakes have a positive effect on the water supply of the neighboring ecosystem. However, information evaluating whether newly built lakes improve the physiochemical properties and microorgani...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.912

    authors: Li J,An C,Shang J,Zhao T,Zhang Q,Yang X,Ren C,Huang D,Liu K,Shao X

    更新日期:2019-12-01 00:00:00

  • The PAS domains of the major sporulation kinase in Bacillus subtilis play a role in tetramer formation that is essential for the autokinase activity.

    abstract::Sporulation in Bacillus subtilis is induced upon starvation. In a widely accepted model, an N-terminal "sensor" domain of the major sporulation kinase KinA recognizes a hypothetical starvation signal(s) and autophosphorylates a histidine residue to activate the master regulator Spo0A via a multicomponent phosphorelay....

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.481

    authors: Kiehler B,Haggett L,Fujita M

    更新日期:2017-08-01 00:00:00

  • A membrane-bound [NiFe]-hydrogenase large subunit precursor whose C-terminal extension is not essential for cofactor incorporation but guarantees optimal maturation.

    abstract::[NiFe]-hydrogenases catalyze the reversible conversion of molecular hydrogen into protons end electrons. This reaction takes place at a NiFe(CN)2 (CO) cofactor located in the large subunit of the bipartite hydrogenase module. The corresponding apo-protein carries usually a C-terminal extension that is cleaved off by a...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.1029

    authors: Hartmann S,Frielingsdorf S,Caserta G,Lenz O

    更新日期:2020-06-01 00:00:00

  • Evolutionary changes of an intestinal Lactobacillus reuteri during probiotic manufacture.

    abstract::Probiotic bacteria are frequently used to treat intestinal diseases or to improve health; however, little is known about the evolutionary changes of these bacteria during probiotic manufacture and the bacterial ability to colonize the intestine. It has been observed that when bacteria adapt to a new environment, they ...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.972

    authors: López L,Calderón D,Cardenas P,Prado MB,Valle C,Trueba G

    更新日期:2020-02-01 00:00:00

  • σ(N) -dependent control of acid resistance and the locus of enterocyte effacement in enterohemorrhagic Escherichia coli is activated by acetyl phosphate in a manner requiring flagellar regulator FlhDC and the σ(S) antagonist FliZ.

    abstract::In enterohemorrhagic Escherichia coli (EHEC), sigma factor N (σ(N)) regulates glutamate-dependent acid resistance (GDAR) and the locus of enterocyte effacement (LEE); discrete genetic systems that are required for transmission and virulence of this intestinal pathogen. Regulation of these systems requires nitrogen reg...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.183

    authors: Mitra A,Fay PA,Vendura KW,Alla Z,Carroll RK,Shaw LN,Riordan JT

    更新日期:2014-08-01 00:00:00

  • Investigating the effect of carbon source on rabies virus glycoprotein production in Pichia pastoris by a transcriptomic approach.

    abstract::Several factors affect protein expression in Pichia pastoris, one among them is the carbon source. In this work, we studied the effect of this factor on the expression level of rabies virus glycoprotein (RABV-G) in two recombinant clones harboring seven copies of the gene of interest. The expression was driven either ...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.489

    authors: Ben Azoun S,Kallel H

    更新日期:2017-08-01 00:00:00

  • New insights into FtsZ rearrangements during the cell division of Escherichia coli from single-molecule localization microscopy of fixed cells.

    abstract::FtsZ - a prokaryotic tubulin homolog - is one of the central components of bacterial division machinery. At the early stage of cytokinesis FtsZ forms the so-called Z-ring at mid-cell that guides septum formation. Many approaches were used to resolve the structure of the Z-ring, however, researchers are still far from ...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.336

    authors: Vedyaykin AD,Vishnyakov IE,Polinovskaya VS,Khodorkovskii MA,Sabantsev AV

    更新日期:2016-06-01 00:00:00

  • Shared and unique microbes between Small hive beetles (Aethina tumida) and their honey bee hosts.

    abstract::The small hive beetle (SHB) is an opportunistic parasite that feeds on bee larvae, honey, and pollen. While SHBs can also feed on fruit and other plant products, like its plant-feeding relatives, SHBs prefer to feed on hive resources and only reproduce inside bee colonies. As parasites, SHBs are inevitably exposed to ...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.899

    authors: Huang Q,Lopez D,Evans JD

    更新日期:2019-10-01 00:00:00