New insights into FtsZ rearrangements during the cell division of Escherichia coli from single-molecule localization microscopy of fixed cells.

Abstract:

:FtsZ - a prokaryotic tubulin homolog - is one of the central components of bacterial division machinery. At the early stage of cytokinesis FtsZ forms the so-called Z-ring at mid-cell that guides septum formation. Many approaches were used to resolve the structure of the Z-ring, however, researchers are still far from consensus on this question. We utilized single-molecule localization microscopy (SMLM) in combination with immunofluorescence staining to visualize FtsZ in Esherichia coli fixed cells that were grown under slow and fast growth conditions. This approach allowed us to obtain images of FtsZ structures at different stages of cell division and accurately measure Z-ring dimensions. Analysis of these images demonstrated that Z-ring thickness increases during constriction, starting at about 70 nm at the beginning of division and increasing by approximately 25% half-way through constriction.

journal_name

Microbiologyopen

journal_title

MicrobiologyOpen

authors

Vedyaykin AD,Vishnyakov IE,Polinovskaya VS,Khodorkovskii MA,Sabantsev AV

doi

10.1002/mbo3.336

subject

Has Abstract

pub_date

2016-06-01 00:00:00

pages

378-86

issue

3

issn

2045-8827

journal_volume

5

pub_type

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