Abstract:
:Bacterial colonization of the urogenital tract is limited by innate defenses, including the production of antimicrobial peptides (AMPs). Uropathogenic Escherichia coli (UPEC) resist AMP-killing to cause a range of urinary tract infections (UTIs) including asymptomatic bacteriuria, cystitis, pyelonephritis, and sepsis. UPEC strains have high genomic diversity and encode numerous virulence factors that differentiate them from non-UTI-causing strains, including ompT. As OmpT homologs cleave and inactivate AMPs, we hypothesized that UPEC strains from patients with symptomatic UTIs have high OmpT protease activity. Therefore, we measured OmpT activity in 58 clinical E. coli isolates. While heterogeneous OmpT activities were observed, OmpT activity was significantly greater in UPEC strains isolated from patients with symptomatic infections. Unexpectedly, UPEC strains exhibiting the greatest protease activities harbored an additional ompT-like gene called arlC (ompTp). The presence of two OmpT-like proteases in some UPEC isolates led us to compare the substrate specificities of OmpT-like proteases found in E. coli. While all three cleaved AMPs, cleavage efficiency varied on the basis of AMP size and secondary structure. Our findings suggest the presence of ArlC and OmpT in the same UPEC isolate may confer a fitness advantage by expanding the range of target substrates.
journal_name
Microbiologyopenjournal_title
MicrobiologyOpenauthors
Desloges I,Taylor JA,Leclerc JM,Brannon JR,Portt A,Spencer JD,Dewar K,Marczynski GT,Manges A,Gruenheid S,Le Moual H,Thomassin JLdoi
10.1002/mbo3.915subject
Has Abstractpub_date
2019-11-01 00:00:00pages
e915issue
11issn
2045-8827journal_volume
8pub_type
杂志文章相关文献
MicrobiologyOpen文献大全abstract::Microbial biofilms are communities of surface-adhered cells enclosed in a matrix of extracellular polymeric substances. Bacterial cells in biofilm are 10~1,000-fold more resistant to antimicrobials than the planktonic cells. Burgeoning antibiotic resistance in Pseudomonas aeruginosa biofilm has necessitated the develo...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.545
更新日期:2018-02-01 00:00:00
abstract::The dynamics of swimming microorganisms is strongly affected by solid-liquid and air-liquid interfaces. In this paper, we characterize the motion of both single bacteria and microcolonies at an air-liquid interface. Both of them follow circular trajectories. Single bacteria preferentially show a counter-clockwise moti...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.532
更新日期:2018-02-01 00:00:00
abstract::Protein lysine acetylation, a dynamic and reversible posttranslational modification, plays a crucial role in several cellular processes, including cell cycle regulation, metabolism, enzymatic activities, and protein interactions. Brenneria nigrifluens is a pathogen of walnut trees with shallow bark canker and can caus...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.952
更新日期:2020-01-01 00:00:00
abstract::Proteins are the most diverse structures on bacterial surfaces; hence, they are candidates for species- and strain-specific interactions of bacteria with the host, environment, and other microorganisms. Genomics has decoded thousands of bacterial surface and secreted proteins, yet the function of most cannot be predic...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.53
更新日期:2013-02-01 00:00:00
abstract::The Hatton-Rockall Basin (North-East Atlantic) is an area with potential for deep-sea (2,900 m) hydrocarbon exploration. Following the Deepwater Horizon oil spill, many investigations into the responses of sediment microbial communities to oil pollution have been undertaken. However, hydrostatic pressure is a paramete...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.768
更新日期:2019-06-01 00:00:00
abstract::The Siamese crocodile (Crocodylus siamensis) is a freshwater, endangered crocodile with high economic value in the farming industry. Gut microflora plays an essential role in host physiological activity, and it contributes significantly to both the health and diseased states of animals. However, thus far, no study has...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.929
更新日期:2019-12-01 00:00:00
abstract::Marine picocyanobacteria belonging to genera Synechococcus and Prochlorococcus are genetically diverged and distributed into distinct biogeographical patterns, and both are infected by genetically closely related cyanopodoviruses. Previous studies have not fully explored whether the two virus-host systems share simila...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.1150
更新日期:2020-12-30 00:00:00
abstract::Pseudomonas aeruginosa and Staphylococcus aureus mixed-species biofilm infections are more resilient to biocide attacks compared to their single-species counterparts. Therefore, this study used an in vitro model recapitulating bacterial burdens seen in in vivo infections to investigate the interactions of P. aeruginos...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.427
更新日期:2017-06-01 00:00:00
abstract::The intraspecies genomic diversity of the single-strand RNA (+) virus species hepatitis A virus (Hepatovirus), hepatitis C virus (Hepacivirus), and hepatitis E virus (Orthohepevirus) was compared. These viral species all can cause liver inflammation (hepatitis), but share no gene similarity. The codon usage of human h...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.973
更新日期:2020-02-01 00:00:00
abstract::Directed enzyme prodrug therapy (DEPT) is a cancer chemotherapy strategy in which bacterial enzymes are delivered to a cancer site before prodrug administration, resulting in prodrug activation at the cancer site and more localized treatment. A major limitation to DEPT is the poor effectiveness of the most studied enz...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.1110
更新日期:2020-10-01 00:00:00
abstract::Soil water content (SWC) is an important determinant for nutrient cycling and microorganism activity in the grassland ecosystem. Lakes have a positive effect on the water supply of the neighboring ecosystem. However, information evaluating whether newly built lakes improve the physiochemical properties and microorgani...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.912
更新日期:2019-12-01 00:00:00
abstract::Avian pathogenic Escherichia coli (APEC) causes airsacculitis, polyserositis, septicemia, and other mainly extraintestinal diseases in chickens, ducks, geese, pigeons, and other avian species, and is responsible for great economic losses in the avian industry. The autoinducer 2 (AI-2) quorum sensing system is widely p...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.582
更新日期:2018-08-01 00:00:00
abstract::The extreme radiation resistance of Deinococcus bacteria requires the radiation-stimulated cleavage of protein DdrO by a specific metalloprotease called IrrE. DdrO is the repressor of a predicted radiation/desiccation response (RDR) regulon, composed of radiation-induced genes having a conserved DNA motif (RDRM) in th...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.477
更新日期:2017-08-01 00:00:00
abstract::The aim of this study was to determine the serogroups, antimicrobial resistance and genetic diversity of Escherichia coli isolates from samples of bivalve mollusks collected along Santa Catarina coast, Brazil, and from the Chesapeake Bay, Maryland, USA. One hundred forty-one E. coli isolates were characterized for ser...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.738
更新日期:2019-05-01 00:00:00
abstract::UV resistance of bacteria isolated from the marine cyanobacterium Moorea producens has not been observed previously, findings which highlight how unsafe germicidal UV irradiation for sterilization of air, food, and water could be. Further, UV resistance of Bacillus licheniformis is being observed for the first time. T...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.697
更新日期:2019-04-01 00:00:00
abstract::Copper resistance system in the cyanobacterium Synechocystis sp. PCC 6803 comprises two operons, copMRS and copBAC, which are expressed in response to copper in the media. copBAC codes for a heavy-metal efflux-resistance nodulation and division (HME-RND) system, while copMRS codes for a protein of unknown function, Co...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.231
更新日期:2015-02-01 00:00:00
abstract::To investigate the possible effects of sulfamonomethoxine (SMM) on Nile tilapia (Oreochromis niloticus), we quantitatively evaluated the microbial shifts in the intestines of Nile tilapia in response to different doses of SMM (200 and 300 mg/kg) using 16S rRNA gene sequencing. At the phylum level, the control group (0...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.1116
更新日期:2020-11-01 00:00:00
abstract::Sourdough fermentation is a traditional process that is used to improve bread quality. A spontaneous sourdough ecosystem consists of a mixture of flour and water that is fermented by endogenous lactic acid bacteria (LAB) and yeasts. The aim of this study was to identify bacterial diversity during backslopping of spont...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.1009
更新日期:2020-04-01 00:00:00
abstract::During mixed-acid fermentation Escherichia coli produces formate, which is initially excreted out the cell. Accumulation of formate, and dropping extracellular pH, leads to biosynthesis of the formate hydrogenlyase (FHL) complex. FHL consists of membrane and soluble domains anchored within the inner membrane. The solu...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.365
更新日期:2016-10-01 00:00:00
abstract::A robust DNA extraction method is important to identify the majority of microorganisms present in environmental microbial communities and to enable a consistent comparison between different studies. Here, 15 manual and four automated commercial DNA extraction kits were evaluated for their efficiency to extract DNA fro...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.312
更新日期:2016-02-01 00:00:00
abstract::The plant growth-promoting rhizobacterium Pseudomonas fluorescens WCS374r produces several iron-regulated metabolites, including the fluorescent siderophore pseudobactin (Psb374), salicylic acid (SA), and pseudomonine (Psm), a siderophore that contains a SA moiety. After purification of Psb374 from culture supernatant...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.32
更新日期:2012-09-01 00:00:00
abstract::In Escherichia coli, coordinated activation and deactivation of DnaA allows for proper timing of the initiation of chromosomal synthesis at the origin of replication (oriC) and assures initiation occurs once per cell cycle. In vitro, acidic phospholipids reactivate DnaA, and in vivo depletion of acidic phospholipids, ...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.46
更新日期:2012-12-01 00:00:00
abstract::Human gut microbiome dysbiosis has been associated with the onset of metabolic diseases and disorders. However, the critical factors leading to dysbiosis are poorly understood. In this study, we provide increasing evidence of the association of diet type and body mass index (BMI) and how they relatively influence the ...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.476
更新日期:2017-08-01 00:00:00
abstract::In rod-shaped bacteria, the proper placement of the division septum at the midcell relies, at least partially, on the proteins of the Min system as an inhibitor of cell division. The main principle of Min system function involves the formation of an inhibitor gradient along the cell axis; however, the establishment of...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.337
更新日期:2016-06-01 00:00:00
abstract::Sucrose is an important disaccharide used as a substrate in many industrial applications. It is a major component of molasses, a cheap by-product of the sugar industry. Unfortunately, not all industrially relevant organisms, among them Pseudomonas putida, are capable of metabolizing sucrose. We chose a metabolic engin...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.473
更新日期:2017-08-01 00:00:00
abstract::Several factors affect protein expression in Pichia pastoris, one among them is the carbon source. In this work, we studied the effect of this factor on the expression level of rabies virus glycoprotein (RABV-G) in two recombinant clones harboring seven copies of the gene of interest. The expression was driven either ...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.489
更新日期:2017-08-01 00:00:00
abstract::The composition and function of the microbial community in the gastrointestinal tract (GIT) have increasingly captured the attention of nutritionists because these traits affect the nutrient utilization efficiency and health of host animals. Little information has been reported on these aspects of the goat GIT. This s...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.820
更新日期:2019-09-01 00:00:00
abstract::We have designed a thermoelectric heat exchanger (TEHE) for microbial fermentations that is able to produce electric power from a microbial continuous culture using the intrinsic heat generated by microbial growth. While the TEHE was connected, the system proved able to stably self-maintain both the temperature and th...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.648
更新日期:2019-03-01 00:00:00
abstract::Although microorganisms play crucial roles in ecosystems, metagenomic analyses of soil samples are quite scarce, especially in the Southern Hemisphere. In this work, the microbial diversity of soil samples from an Atlantic Forest and Caatinga was analyzed using a metagenomic approach. Proteobacteria and Actinobacteria...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.169
更新日期:2014-06-01 00:00:00
abstract::Microbial communities in sediments play an important role in alpine lake ecosystems. However, the microbial diversity and community composition of alpine lake sediments from the Hengduan Mountains remain largely unknown. Therefore, based on the Illumina MiSeq platform, high-throughput sequencing analysis of the 16S rR...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.832
更新日期:2019-09-01 00:00:00