Identification and characterization of OmpT-like proteases in uropathogenic Escherichia coli clinical isolates.

Abstract:

:Bacterial colonization of the urogenital tract is limited by innate defenses, including the production of antimicrobial peptides (AMPs). Uropathogenic Escherichia coli (UPEC) resist AMP-killing to cause a range of urinary tract infections (UTIs) including asymptomatic bacteriuria, cystitis, pyelonephritis, and sepsis. UPEC strains have high genomic diversity and encode numerous virulence factors that differentiate them from non-UTI-causing strains, including ompT. As OmpT homologs cleave and inactivate AMPs, we hypothesized that UPEC strains from patients with symptomatic UTIs have high OmpT protease activity. Therefore, we measured OmpT activity in 58 clinical E. coli isolates. While heterogeneous OmpT activities were observed, OmpT activity was significantly greater in UPEC strains isolated from patients with symptomatic infections. Unexpectedly, UPEC strains exhibiting the greatest protease activities harbored an additional ompT-like gene called arlC (ompTp). The presence of two OmpT-like proteases in some UPEC isolates led us to compare the substrate specificities of OmpT-like proteases found in E. coli. While all three cleaved AMPs, cleavage efficiency varied on the basis of AMP size and secondary structure. Our findings suggest the presence of ArlC and OmpT in the same UPEC isolate may confer a fitness advantage by expanding the range of target substrates.

journal_name

Microbiologyopen

journal_title

MicrobiologyOpen

authors

Desloges I,Taylor JA,Leclerc JM,Brannon JR,Portt A,Spencer JD,Dewar K,Marczynski GT,Manges A,Gruenheid S,Le Moual H,Thomassin JL

doi

10.1002/mbo3.915

subject

Has Abstract

pub_date

2019-11-01 00:00:00

pages

e915

issue

11

issn

2045-8827

journal_volume

8

pub_type

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