Abstract:
:The extreme radiation resistance of Deinococcus bacteria requires the radiation-stimulated cleavage of protein DdrO by a specific metalloprotease called IrrE. DdrO is the repressor of a predicted radiation/desiccation response (RDR) regulon, composed of radiation-induced genes having a conserved DNA motif (RDRM) in their promoter regions. Here, we showed that addition of zinc ions to purified apo-IrrE, and short exposure of Deinococcus cells to zinc ions, resulted in cleavage of DdrO in vitro and in vivo, respectively. Binding of IrrE to RDRM-containing DNA or interaction of IrrE with DNA-bound DdrO was not observed. The data are in line with IrrE being a zinc peptidase, and indicate that increased zinc availability, caused by oxidative stress, triggers the in vivo cleavage of DdrO unbound to DNA. Transcriptomics and proteomics of Deinococcus deserti confirmed the IrrE-dependent regulation of predicted RDR regulon genes and also revealed additional members of this regulon. Comparative analysis showed that the RDR regulon is largely well conserved in Deinococcus species, but also showed diversity in the regulon composition. Notably, several RDR genes with an important role in radiation resistance in Deinococcus radiodurans, for example pprA, are not conserved in some other radiation-resistant Deinococcus species.
journal_name
Microbiologyopenjournal_title
MicrobiologyOpenauthors
Blanchard L,Guérin P,Roche D,Cruveiller S,Pignol D,Vallenet D,Armengaud J,de Groot Adoi
10.1002/mbo3.477subject
Has Abstractpub_date
2017-08-01 00:00:00issue
4issn
2045-8827journal_volume
6pub_type
杂志文章相关文献
MicrobiologyOpen文献大全abstract::A yeast expression plasmid was constructed containing a cardenolide biosynthetic module, referred to as CARD II, using the AssemblX toolkit, which enables the assembly of large DNA constructs. The genes cloned into the vector were (a) a Δ5 -3β-hydroxysteroid dehydrogenase gene from Digitalis lanata, (b) a steroid Δ5 -...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.925
更新日期:2019-12-01 00:00:00
abstract::[NiFe]-hydrogenases catalyze the reversible conversion of molecular hydrogen into protons end electrons. This reaction takes place at a NiFe(CN)2 (CO) cofactor located in the large subunit of the bipartite hydrogenase module. The corresponding apo-protein carries usually a C-terminal extension that is cleaved off by a...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.1029
更新日期:2020-06-01 00:00:00
abstract::Marine picocyanobacteria belonging to genera Synechococcus and Prochlorococcus are genetically diverged and distributed into distinct biogeographical patterns, and both are infected by genetically closely related cyanopodoviruses. Previous studies have not fully explored whether the two virus-host systems share simila...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.1150
更新日期:2020-12-30 00:00:00
abstract::The intraspecies genomic diversity of the single-strand RNA (+) virus species hepatitis A virus (Hepatovirus), hepatitis C virus (Hepacivirus), and hepatitis E virus (Orthohepevirus) was compared. These viral species all can cause liver inflammation (hepatitis), but share no gene similarity. The codon usage of human h...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.973
更新日期:2020-02-01 00:00:00
abstract::Betaproteobacteria were the most common isolates from the water-filled tank of a Costa Rican bromeliad. Isolates included eight species from the orders Neisseriales and Burkholderiales, with close relatives recovered previously from tropical soils, wetlands, freshwater, or in association with plants. Compared to close...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.344
更新日期:2016-06-01 00:00:00
abstract::In Escherichia coli, coordinated activation and deactivation of DnaA allows for proper timing of the initiation of chromosomal synthesis at the origin of replication (oriC) and assures initiation occurs once per cell cycle. In vitro, acidic phospholipids reactivate DnaA, and in vivo depletion of acidic phospholipids, ...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.46
更新日期:2012-12-01 00:00:00
abstract::FtsZ - a prokaryotic tubulin homolog - is one of the central components of bacterial division machinery. At the early stage of cytokinesis FtsZ forms the so-called Z-ring at mid-cell that guides septum formation. Many approaches were used to resolve the structure of the Z-ring, however, researchers are still far from ...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.336
更新日期:2016-06-01 00:00:00
abstract::Pseudomonas aeruginosa is a good environmental microorganism capable of degrading decabromodiphenyl ether (BDE-209). This paper studied the effect of Cu2+ and humic acid (HA) extracted from e-waste contaminated soils on biodegradation of BDE-209 by P. aeruginosa. The adsorption isotherms of Cu2+ on HA, the crude enzym...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.439
更新日期:2017-06-01 00:00:00
abstract::Metal- and pesticide-tolerant biocontrol agents are preferred in integrated pest management, as such strains can be applied in combination with different pesticides. The Bacillus velezensis strain SZMC 6161J proved to be sensitive to copper, nickel, zinc, and cadmium, while manganese elevated its growth. At concentrat...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.660
更新日期:2019-03-01 00:00:00
abstract::In rod-shaped bacteria, the proper placement of the division septum at the midcell relies, at least partially, on the proteins of the Min system as an inhibitor of cell division. The main principle of Min system function involves the formation of an inhibitor gradient along the cell axis; however, the establishment of...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.337
更新日期:2016-06-01 00:00:00
abstract::The effect of elevated atmospheric CO2 concentration [CO2 ] on the diversity and composition of the prokaryotic community inhabiting the rhizosphere of winter barley (Hordeum vulgare L.) was investigated in a field experiment, using open-top chambers. Rhizosphere samples were collected at anthesis (flowering stage) fr...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.462
更新日期:2017-08-01 00:00:00
abstract::Microorganisms require a motility structure to move towards optimal growth conditions. The motility structure from archaea, the archaellum, is fundamentally different from its bacterial counterpart, the flagellum, and is assembled in a similar fashion as type IV pili. The archaellum filament consists of thousands of c...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.718
更新日期:2019-05-01 00:00:00
abstract::Sourdough fermentation is a traditional process that is used to improve bread quality. A spontaneous sourdough ecosystem consists of a mixture of flour and water that is fermented by endogenous lactic acid bacteria (LAB) and yeasts. The aim of this study was to identify bacterial diversity during backslopping of spont...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.1009
更新日期:2020-04-01 00:00:00
abstract::Genomic analysis points to N-glycosylation as being a common posttranslational modification in Archaea. To date, however, pathways of archaeal N-glycosylation have only been described for few species. With this in mind, the similarities of N-linked glycans decorating glycoproteins in the haloarchaea Haloferax volcanii...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.215
更新日期:2015-02-01 00:00:00
abstract::Most DNA assembly methods require bacterial amplification steps, which restrict its application to genes that can be cloned in the bacterial host without significant toxic effects. However, genes that cannot be cloned in bacteria do not necessarily exert toxic effects on the final host. In order to tackle this issue, ...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.978
更新日期:2020-03-01 00:00:00
abstract::Dinitrogen fixation, the biological reduction in N2 gas to ammonia contributes to the supply of new nitrogen in the surface ocean. To understand the diversity and abundance of potentially diazotrophic (N2 fixing) microorganisms associated with marine zooplankton, especially copepods, the nifH gene was studied using zo...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.385
更新日期:2016-12-01 00:00:00
abstract::RodZ is important for maintaining the rod shape of Escherichia coli. Loss of RodZ causes conversion of the rod shape to a round shape and a growth rate slower than that of wild-type cells. Suppressor mutations that simultaneously restore both the growth rates and the rod shape were isolated. Most of the suppressor mut...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.116
更新日期:2013-10-01 00:00:00
abstract::Increasing popularity of preservative-free cosmetics necessitates in-depth research, specifically as bacteria can react to local factors by important metabolic changes. In this respect, investigating the effect of cosmetic preparations on pathogenic strains of commensal species such as acneic forms of Cutibacterium ac...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.659
更新日期:2019-03-01 00:00:00
abstract::UV resistance of bacteria isolated from the marine cyanobacterium Moorea producens has not been observed previously, findings which highlight how unsafe germicidal UV irradiation for sterilization of air, food, and water could be. Further, UV resistance of Bacillus licheniformis is being observed for the first time. T...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.697
更新日期:2019-04-01 00:00:00
abstract::Pseudomonas aeruginosa is a Gram negative, opportunistic pathogen that uses the overproduction of alginate, a surface polysaccharide, to form biofilms in vivo. Overproduction of alginate, also known as mucoidy, affords the bacterium protection from the host's defenses and facilitates the establishment of chronic lung ...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.86
更新日期:2013-06-01 00:00:00
abstract:BACKGROUND:The microbiome is an integral component of many animal species, potentially affecting behavior, physiology, and other biological properties. Despite this importance, bacterial communities remain vastly understudied in many groups of invertebrates, including mites. Quill mites (Acariformes: Syringophilidae) a...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.964
更新日期:2020-05-01 00:00:00
abstract::This study compared the sensitivity of spores from virulent and attenuated Bacillus anthracis strains in suspension to inactivation by various chemical disinfectants. Spore suspensions from two virulent strains (A0256 and A0372) and two attenuated strains (Sterne and A0141) of B. anthracis were tested against two alde...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.45
更新日期:2012-12-01 00:00:00
abstract::Avian pathogenic Escherichia coli (APEC) causes airsacculitis, polyserositis, septicemia, and other mainly extraintestinal diseases in chickens, ducks, geese, pigeons, and other avian species, and is responsible for great economic losses in the avian industry. The autoinducer 2 (AI-2) quorum sensing system is widely p...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.582
更新日期:2018-08-01 00:00:00
abstract::Type II toxin-antitoxin (TA) modules are thought to mediate stress-responses by temporarily suppressing protein synthesis while cells redirect transcription to adapt to environmental change. Here, we show that YoeB, a ribosome-dependent mRNase toxin, is activated in Escherichia coli cells grown at elevated temperature...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.272
更新日期:2015-08-01 00:00:00
abstract::Vibrio coralliilyticus is an important coral pathogen demonstrated to cause disease outbreaks worldwide. This study investigated the feasibility of applying bacteriophage therapy to treat the coral pathogen V. coralliilyticus. A specific bacteriophage for V. coralliilyticus strain P1 (LMG23696), referred to here as ba...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.52
更新日期:2013-02-01 00:00:00
abstract::Aeromonas caviae is motile via a polar flagellum in liquid culture, with a lateral flagella system used for swarming on solid surfaces. The polar flagellum also has a role in cellular adherence and biofilm formation. The two subunits of the polar flagellum, FlaA and FlaB, are posttranslationally modified by O-linked g...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.233
更新日期:2015-04-01 00:00:00
abstract::A new type of structural extracellular polymers (EPS) was extracted from aerobic granular sludge dominated by ammonium-oxidizing bacteria. It was analyzed by Raman and FTIR spectroscopy to characterize specific amino acids and protein secondary structure, and by SDS-PAGE with different stains to identify different gly...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.616
更新日期:2018-12-01 00:00:00
abstract::Brazil is the biggest producer of sweet oranges and the main exporter of concentrated orange juice in the world. Among the diseases that affect citriculture, Asiatic citrus canker, caused by the bacterial pathogen Xanthomonas citri, represents one of the most significant threats. The current Brazilian legislation regu...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.1104
更新日期:2020-09-01 00:00:00
abstract::Bacterial community structures were evaluated in oil samples using culture-independent pyrosequencing, including oil mousses collected on sea surface and salt marshes during the Deepwater Horizon oil spill, and oil deposited in sediments adjacent to the wellhead 1 year after the spill. Phylogenetic analysis suggested ...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.89
更新日期:2013-06-01 00:00:00
abstract:AIMS:To assess a cost-effective in-house selective plate formula for actively screening carbapenem-resistant Enterobacteriaceae (CRE). METHODOLOGY AND RESULTS:The in-house formula included CHROMagarTM Orientation, meropenem, and ingredients present in the Mac-Conkey formula, such as bile salts and crystal violet (pH 6...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.1130
更新日期:2020-12-01 00:00:00