Possible association of diazotrophs with marine zooplankton in the Pacific Ocean.

Abstract:

:Dinitrogen fixation, the biological reduction in N2 gas to ammonia contributes to the supply of new nitrogen in the surface ocean. To understand the diversity and abundance of potentially diazotrophic (N2 fixing) microorganisms associated with marine zooplankton, especially copepods, the nifH gene was studied using zooplankton samples collected in the Pacific Ocean. In total, 257 nifH sequences were recovered from 23 nifH-positive DNA extracts out of 90 copepod samples. The nifH genes derived from cyanobacteria related to Trichodesmium, α- and γ-subdivisions of proteobacteria, and anaerobic euryarchaeota related to Methanosaeta concilii were detected. Our results indicated that Pleuromamma, Pontella, and Euchaeta were the major copepod genera hosting dinitrogen fixers, though we found no species-specific association between copepods and dinitrogen fixers. Also, the digital PCR provided novel data on the number of copies of the nifH gene in individual copepods, which we report the range from 30 to 1666 copies per copepod. This study is the first systematic study of zooplankton-associated diazotrophs, covering a large area of the open ocean, which provide a clue to further study of a possible new hotspot of N2 fixation.

journal_name

Microbiologyopen

journal_title

MicrobiologyOpen

authors

Azimuddin KM,Hirai J,Suzuki S,Haider MN,Tachibana A,Watanabe K,Kitamura M,Hashihama F,Takahashi K,Hamasaki K

doi

10.1002/mbo3.385

subject

Has Abstract

pub_date

2016-12-01 00:00:00

pages

1016-1026

issue

6

issn

2045-8827

journal_volume

5

pub_type

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