Genetic determinants of swimming motility in the squid light-organ symbiont Vibrio fischeri.

Abstract:

:Bacterial flagellar motility is a complex cellular behavior required for the colonization of the light-emitting organ of the Hawaiian bobtail squid, Euprymna scolopes, by the beneficial bioluminescent symbiont Vibrio fischeri. We characterized the basis of this behavior by performing (i) a forward genetic screen to identify mutants defective in soft-agar motility, as well as (ii) a transcriptional analysis to determine the genes that are expressed downstream of the flagellar master regulator FlrA. Mutants with severe defects in soft-agar motility were identified due to insertions in genes with putative roles in flagellar motility and in genes that were unexpected, including those predicted to encode hypothetical proteins and cell division-related proteins. Analysis of mutants for their ability to enter into a productive symbiosis indicated that flagellar motility mutants are deficient, while chemotaxis mutants are able to colonize a subset of juvenile squid to light-producing levels. Thirty-three genes required for normal motility in soft agar were also downregulated in the absence of FlrA, suggesting they belong to the flagellar regulon of V. fischeri. Mutagenesis of putative paralogs of the flagellar motility genes motA, motB, and fliL revealed that motA1, motB1, and both fliL1 and fliL2, but not motA2 and motB2, likely contribute to soft-agar motility. Using these complementary approaches, we have characterized the genetic basis of flagellar motility in V. fischeri and furthered our understanding of the roles of flagellar motility and chemotaxis in colonization of the juvenile squid, including identifying 11 novel mutants unable to enter into a productive light-organ symbiosis.

journal_name

Microbiologyopen

journal_title

MicrobiologyOpen

authors

Brennan CA,Mandel MJ,Gyllborg MC,Thomasgard KA,Ruby EG

doi

10.1002/mbo3.96

subject

Has Abstract

pub_date

2013-08-01 00:00:00

pages

576-94

issue

4

issn

2045-8827

journal_volume

2

pub_type

杂志文章
  • Possible association of diazotrophs with marine zooplankton in the Pacific Ocean.

    abstract::Dinitrogen fixation, the biological reduction in N2 gas to ammonia contributes to the supply of new nitrogen in the surface ocean. To understand the diversity and abundance of potentially diazotrophic (N2 fixing) microorganisms associated with marine zooplankton, especially copepods, the nifH gene was studied using zo...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.385

    authors: Azimuddin KM,Hirai J,Suzuki S,Haider MN,Tachibana A,Watanabe K,Kitamura M,Hashihama F,Takahashi K,Hamasaki K

    更新日期:2016-12-01 00:00:00

  • Systematic analysis of the kalimantacin assembly line NRPS module using an adapted targeted mutagenesis approach.

    abstract::Kalimantacin is an antimicrobial compound with strong antistaphylococcal activity that is produced by a hybrid trans-acyltransferase polyketide synthase/nonribosomal peptide synthetase system in Pseudomonas fluorescens BCCM_ID9359. We here present a systematic analysis of the substrate specificity of the glycine-incor...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.326

    authors: Uytterhoeven B,Appermans K,Song L,Masschelein J,Lathouwers T,Michiels CW,Lavigne R

    更新日期:2016-04-01 00:00:00

  • σ(N) -dependent control of acid resistance and the locus of enterocyte effacement in enterohemorrhagic Escherichia coli is activated by acetyl phosphate in a manner requiring flagellar regulator FlhDC and the σ(S) antagonist FliZ.

    abstract::In enterohemorrhagic Escherichia coli (EHEC), sigma factor N (σ(N)) regulates glutamate-dependent acid resistance (GDAR) and the locus of enterocyte effacement (LEE); discrete genetic systems that are required for transmission and virulence of this intestinal pathogen. Regulation of these systems requires nitrogen reg...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.183

    authors: Mitra A,Fay PA,Vendura KW,Alla Z,Carroll RK,Shaw LN,Riordan JT

    更新日期:2014-08-01 00:00:00

  • Microbial community composition in alpine lake sediments from the Hengduan Mountains.

    abstract::Microbial communities in sediments play an important role in alpine lake ecosystems. However, the microbial diversity and community composition of alpine lake sediments from the Hengduan Mountains remain largely unknown. Therefore, based on the Illumina MiSeq platform, high-throughput sequencing analysis of the 16S rR...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.832

    authors: Liao B,Yan X,Zhang J,Chen M,Li Y,Huang J,Lei M,He H,Wang J

    更新日期:2019-09-01 00:00:00

  • A novel gingipain regulatory gene in Porphyromonas gingivalis mediates host cell detachment and inhibition of wound closure.

    abstract::The black pigmentation-related genes in Porphyromonas gingivalis are primarily involved in regulating gingipain functions. In this study, we identified a pigmentation-related gene, designated as pgn_0361. To characterize the role of pgn_0361 in regulating P. gingivalis-mediated epithelial cell detachment and inhibitio...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.1128

    authors: Liu H,Huang L,Cai Y,Bikker FJ,Wei X,Mei Deng D

    更新日期:2020-12-01 00:00:00

  • Oscillating behavior of Clostridium difficile Min proteins in Bacillus subtilis.

    abstract::In rod-shaped bacteria, the proper placement of the division septum at the midcell relies, at least partially, on the proteins of the Min system as an inhibitor of cell division. The main principle of Min system function involves the formation of an inhibitor gradient along the cell axis; however, the establishment of...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.337

    authors: Makroczyová J,Jamroškovič J,Krascsenitsová E,Labajová N,Barák I

    更新日期:2016-06-01 00:00:00

  • XatA, an AT-1 autotransporter important for the virulence of Xylella fastidiosa Temecula1.

    abstract::Xylella fastidiosa Temecula1 is the causative agent of Pierce's disease of grapevine, which is spread by xylem-feeding insects. An important feature of the infection cycle is the ability of X. fastidiosa to colonize and interact with two distinct environments, the xylem of susceptible plants and the insect foregut. He...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.6

    authors: Matsumoto A,Huston SL,Killiny N,Igo MM

    更新日期:2012-03-01 00:00:00

  • AcuC, a histone deacetylase, contributes to the pathogenicity of Aeromonas hydrophila.

    abstract::The interactions of pathogens and phagocytes are complex. Our study demonstrated that Aeromonas hydrophila B11 can survive in the macrophagocytes of Tilapia mossambica. To explore the regulatory processes of A. hydrophila survival in the macrophagocytes, we used the mini-Tn10 transposon mutagenesis system to build a m...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.468

    authors: Jiang Q,Chen W,Qin Y,Huang L,Xu X,Zhao L,Yan Q

    更新日期:2017-08-01 00:00:00

  • Immunogenicity of a bivalent protein as a vaccine against Edwardsiella anguillarum and Vibrio vulnificus in Japanese eel (Anguilla japonica).

    abstract::The OMPs A (OmpA)-of Edwardsiella anguillarum and OmpU of V. vulnificus have been proven to be good antigens. In this study, after construction of a vector, a new recombinant Omp (rOMP) containing both OmpA and OmpU was expressed and purified. Then, the Japanese eels (Anguilla japonica) were intraperitoneally (i.p.) i...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.766

    authors: Guo S,Hu L,Feng J,Lin P,He L,Yan Q

    更新日期:2019-06-01 00:00:00

  • Acetylome analysis of lysine acetylation in the plant pathogenic bacterium Brenneria nigrifluens.

    abstract::Protein lysine acetylation, a dynamic and reversible posttranslational modification, plays a crucial role in several cellular processes, including cell cycle regulation, metabolism, enzymatic activities, and protein interactions. Brenneria nigrifluens is a pathogen of walnut trees with shallow bark canker and can caus...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.952

    authors: Li Y,Xue H,Bian DR,Xu G,Piao C

    更新日期:2020-01-01 00:00:00

  • Meloxicam inhibits biofilm formation and enhances antimicrobial agents efficacy by Pseudomonas aeruginosa.

    abstract::Microbial biofilms are communities of surface-adhered cells enclosed in a matrix of extracellular polymeric substances. Bacterial cells in biofilm are 10~1,000-fold more resistant to antimicrobials than the planktonic cells. Burgeoning antibiotic resistance in Pseudomonas aeruginosa biofilm has necessitated the develo...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.545

    authors: She P,Wang Y,Luo Z,Chen L,Tan R,Wang Y,Wu Y

    更新日期:2018-02-01 00:00:00

  • Effects of cosmetics on the skin microbiome of facial cheeks with different hydration levels.

    abstract::Basic cosmetics was used by volunteers belonging to high (HHG) and low (LHG) hydration groups for 4 weeks, and bacterial communities and biophysical parameters in facial skin were analyzed. Hydration level increases and transepidermal water loss and roughness decreases were observed in both groups after cosmetic use. ...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.557

    authors: Lee HJ,Jeong SE,Lee S,Kim S,Han H,Jeon CO

    更新日期:2018-04-01 00:00:00

  • Biosynthetic approach to combine the first steps of cardenolide formation in Saccharomyces cerevisiae.

    abstract::A yeast expression plasmid was constructed containing a cardenolide biosynthetic module, referred to as CARD II, using the AssemblX toolkit, which enables the assembly of large DNA constructs. The genes cloned into the vector were (a) a Δ5 -3β-hydroxysteroid dehydrogenase gene from Digitalis lanata, (b) a steroid Δ5 -...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.925

    authors: Rieck C,Geiger D,Munkert J,Messerschmidt K,Petersen J,Strasser J,Meitinger N,Kreis W

    更新日期:2019-12-01 00:00:00

  • New insights into FtsZ rearrangements during the cell division of Escherichia coli from single-molecule localization microscopy of fixed cells.

    abstract::FtsZ - a prokaryotic tubulin homolog - is one of the central components of bacterial division machinery. At the early stage of cytokinesis FtsZ forms the so-called Z-ring at mid-cell that guides septum formation. Many approaches were used to resolve the structure of the Z-ring, however, researchers are still far from ...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.336

    authors: Vedyaykin AD,Vishnyakov IE,Polinovskaya VS,Khodorkovskii MA,Sabantsev AV

    更新日期:2016-06-01 00:00:00

  • Posttranslational modification of a vanadium nitrogenase.

    abstract::In microbes that fix nitrogen, nitrogenase catalyzes the conversion of N2 to ammonia in an ATP-demanding reaction. To help conserve energy some bacteria inhibit nitrogenase activity upon exposure to ammonium. The purple nonsulfur phototrophic bacterium Rhodopseudomonas palustris strain CGA009 can synthesize three func...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.265

    authors: Heiniger EK,Harwood CS

    更新日期:2015-08-01 00:00:00

  • Pressure and temperature effects on deep-sea hydrocarbon-degrading microbial communities in subarctic sediments.

    abstract::The Hatton-Rockall Basin (North-East Atlantic) is an area with potential for deep-sea (2,900 m) hydrocarbon exploration. Following the Deepwater Horizon oil spill, many investigations into the responses of sediment microbial communities to oil pollution have been undertaken. However, hydrostatic pressure is a paramete...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.768

    authors: Perez Calderon LJ,Gontikaki E,Potts LD,Shaw S,Gallego A,Anderson JA,Witte U

    更新日期:2019-06-01 00:00:00

  • Depletion of acidic phospholipids influences chromosomal replication in Escherichia coli.

    abstract::In Escherichia coli, coordinated activation and deactivation of DnaA allows for proper timing of the initiation of chromosomal synthesis at the origin of replication (oriC) and assures initiation occurs once per cell cycle. In vitro, acidic phospholipids reactivate DnaA, and in vivo depletion of acidic phospholipids, ...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.46

    authors: Fingland N,Flåtten I,Downey CD,Fossum-Raunehaug S,Skarstad K,Crooke E

    更新日期:2012-12-01 00:00:00

  • Phage therapy treatment of the coral pathogen Vibrio coralliilyticus.

    abstract::Vibrio coralliilyticus is an important coral pathogen demonstrated to cause disease outbreaks worldwide. This study investigated the feasibility of applying bacteriophage therapy to treat the coral pathogen V. coralliilyticus. A specific bacteriophage for V. coralliilyticus strain P1 (LMG23696), referred to here as ba...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.52

    authors: Cohen Y,Joseph Pollock F,Rosenberg E,Bourne DG

    更新日期:2013-02-01 00:00:00

  • Glycosylated amyloid-like proteins in the structural extracellular polymers of aerobic granular sludge enriched with ammonium-oxidizing bacteria.

    abstract::A new type of structural extracellular polymers (EPS) was extracted from aerobic granular sludge dominated by ammonium-oxidizing bacteria. It was analyzed by Raman and FTIR spectroscopy to characterize specific amino acids and protein secondary structure, and by SDS-PAGE with different stains to identify different gly...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.616

    authors: Lin Y,Reino C,Carrera J,Pérez J,van Loosdrecht MCM

    更新日期:2018-12-01 00:00:00

  • Comparative genomics of hepatitis A virus, hepatitis C virus, and hepatitis E virus provides insights into the evolutionary history of Hepatovirus species.

    abstract::The intraspecies genomic diversity of the single-strand RNA (+) virus species hepatitis A virus (Hepatovirus), hepatitis C virus (Hepacivirus), and hepatitis E virus (Orthohepevirus) was compared. These viral species all can cause liver inflammation (hepatitis), but share no gene similarity. The codon usage of human h...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.973

    authors: Wassenaar TM,Jun SR,Robeson M,Ussery DW

    更新日期:2020-02-01 00:00:00

  • UV Resistance of bacteria from the Kenyan Marine cyanobacterium Moorea producens.

    abstract::UV resistance of bacteria isolated from the marine cyanobacterium Moorea producens has not been observed previously, findings which highlight how unsafe germicidal UV irradiation for sterilization of air, food, and water could be. Further, UV resistance of Bacillus licheniformis is being observed for the first time. T...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.697

    authors: Dzeha T,Nyiro C,Kardasopoulos D,Mburu D,Mwafaida J,Hall MJ,Burgess JG

    更新日期:2019-04-01 00:00:00

  • Comparative analysis of early immune responses induced by two strains of Newcastle disease virus in chickens.

    abstract::Newcastle disease, caused by virulent strain of Newcastle disease virus (NDV), is an acute, highly contagious disease that is prevalent worldwide and is responsible for serious economic losses to the poultry industry. In the current study, we compared the early immune responses in chickens infected with two strains of...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.701

    authors: Zhang T,Ren M,Liu C,Xu L,Wang F,Han Z,Shao Y,Ma D

    更新日期:2019-04-01 00:00:00

  • Genomic integration of unclonable gene expression cassettes in Saccharomyces cerevisiae using rapid cloning-free workflows.

    abstract::Most DNA assembly methods require bacterial amplification steps, which restrict its application to genes that can be cloned in the bacterial host without significant toxic effects. However, genes that cannot be cloned in bacteria do not necessarily exert toxic effects on the final host. In order to tackle this issue, ...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.978

    authors: Cataldo VF,Salgado V,Saa PA,Agosin E

    更新日期:2020-03-01 00:00:00

  • Bacterial-like nitric oxide synthase in the haloalkaliphilic archaeon Natronomonas pharaonis.

    abstract::Bacterial nitric oxide (NO) synthases (bNOS) play diverse and important roles in microbial physiology, stress resistance, and virulence. Although bacterial and mammalian NOS enzymes have been well-characterized, comparatively little is known about the prevalence and function of NOS enzymes in Archaea. Analysis of arch...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.1124

    authors: Orsini SS,James KL,Reyes DJ,Couto-Rodriguez RL,Gulko MK,Witte A,Carroll RK,Rice KC

    更新日期:2020-11-01 00:00:00

  • The role of compatible solutes in desiccation resistance of Acinetobacter baumannii.

    abstract::Acinetobacter baumannii is a nosocomial pathogen which can persist in the hospital environment not only due to the acquirement of multiple antibiotic resistances, but also because of its exceptional resistance against disinfectants and desiccation. A suitable desiccation assay was established in which A. baumannii ATC...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.740

    authors: Zeidler S,Müller V

    更新日期:2019-05-01 00:00:00

  • Spatial patterns in soil physicochemical and microbiological properties in a grassland adjacent to a newly built lake.

    abstract::Soil water content (SWC) is an important determinant for nutrient cycling and microorganism activity in the grassland ecosystem. Lakes have a positive effect on the water supply of the neighboring ecosystem. However, information evaluating whether newly built lakes improve the physiochemical properties and microorgani...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.912

    authors: Li J,An C,Shang J,Zhao T,Zhang Q,Yang X,Ren C,Huang D,Liu K,Shao X

    更新日期:2019-12-01 00:00:00

  • Assessment of carbapenem-resistant Enterobacteriaceae-plate formula and quality control procedure.

    abstract:AIMS:To assess a cost-effective in-house selective plate formula for actively screening carbapenem-resistant Enterobacteriaceae (CRE). METHODOLOGY AND RESULTS:The in-house formula included CHROMagarTM Orientation, meropenem, and ingredients present in the Mac-Conkey formula, such as bile salts and crystal violet (pH 6...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.1130

    authors: Tran TC,Pham BT,Pham VH,Ngo TA,Hanberger H,Larsson M,Olson L

    更新日期:2020-12-01 00:00:00

  • A membrane-bound [NiFe]-hydrogenase large subunit precursor whose C-terminal extension is not essential for cofactor incorporation but guarantees optimal maturation.

    abstract::[NiFe]-hydrogenases catalyze the reversible conversion of molecular hydrogen into protons end electrons. This reaction takes place at a NiFe(CN)2 (CO) cofactor located in the large subunit of the bipartite hydrogenase module. The corresponding apo-protein carries usually a C-terminal extension that is cleaved off by a...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.1029

    authors: Hartmann S,Frielingsdorf S,Caserta G,Lenz O

    更新日期:2020-06-01 00:00:00

  • Glycolipid composition of the heterocyst envelope of Anabaena sp. PCC 7120 is crucial for diazotrophic growth and relies on the UDP-galactose 4-epimerase HgdA.

    abstract::The nitrogenase complex in the heterocysts of the filamentous freshwater cyanobacterium Anabaenasp. PCC 7120 fixes atmospheric nitrogen to allow diazotrophic growth. The heterocyst cell envelope protects the nitrogenase from oxygen and consists of a polysaccharide and a glycolipid layer that are formed by a complex pr...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.811

    authors: Shvarev D,Nishi CN,Maldener I

    更新日期:2019-08-01 00:00:00

  • Effect of two cosmetic compounds on the growth, biofilm formation activity, and surface properties of acneic strains of Cutibacterium acnes and Staphylococcus aureus.

    abstract::Increasing popularity of preservative-free cosmetics necessitates in-depth research, specifically as bacteria can react to local factors by important metabolic changes. In this respect, investigating the effect of cosmetic preparations on pathogenic strains of commensal species such as acneic forms of Cutibacterium ac...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.659

    authors: Gannesen AV,Borrel V,Lefeuvre L,Netrusov AI,Plakunov VK,Feuilloley MGJ

    更新日期:2019-03-01 00:00:00